Detection of rare cells at a frequency of one per million by flow cytometry

In a model study we seeded the pre‐B‐cell line REH into 250 million peripheral blood mononuclear cells (PBMC) at frequencies of 10−4, 10−5, and 10−6. By flow cytometry we could detect the REH cells and found a background of about one event per 100 million PBMC. This sensitivity was achieved by remov...

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