Modulation of gastric mucosal calcium channel activity by mucus glycoprotein
1. 1. The effect of gastric mucus glycoprotein on the activity of calcium channel isolated from gastric epithelial cell membrane was investigated. The 45Ca 2+ uptake into the vesicle-reconstituted channels, while only moderately (14%) affected by the intact mucus glycoprotein, was found significantl...
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| Veröffentlicht in: | International journal of biochemistry 1993-06, Vol.25 (6), p.869-878 |
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| container_title | International journal of biochemistry |
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| creator | Slomiany, B.L. Murty, V.L.N. Liu, J. Piotrowski, J. Czajkowski, A. Slomiany, A. |
| description | 1.
1. The effect of gastric mucus glycoprotein on the activity of calcium channel isolated from gastric epithelial cell membrane was investigated. The
45Ca
2+ uptake into the vesicle-reconstituted channels, while only moderately (14%) affected by the intact mucus glycoprotein, was found significantly inhibited (59%) by the acidic glycoprotein fraction. This effect was associated with the sialic acid and sulfate ester groups of the glycoprotein, as their removal caused a loss in the inhibition.
2.
2. The channel complex in the presence of epidermal growth factor (EGF) and ATP responded by an increase in protein tyrosine phosphorylation of 55 and 170 kDa proteins, and the vesicles containing the phosphorylated channels showed a 50% increase in
45Ca
2+ uptake. The phosphorylation and the calcium uptake were susceptible to inhibition by a specific tyrosine kinase inhibitor, genistein.
3.
3. The channel protein phosphorylation was inhibited by the acidic mucus glycoprotein, which also interfered with the binding of EGF to the channel protein. The inhibitory effect was dependent upon the presence of sulfate ester and sialic acid groups, as evidenced by the loss of the glycoprotein inhibitory capacity following their removal.
4.
4. The results suggest that the acidic gastric mucus glycoproteins, by modulating the EGF-controlled calcium channel phosphorylation, play a major role in gastric mucosal calcium homeostasis. |
| doi_str_mv | 10.1016/0020-711X(93)90242-7 |
| format | Article |
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1. The effect of gastric mucus glycoprotein on the activity of calcium channel isolated from gastric epithelial cell membrane was investigated. The
45Ca
2+ uptake into the vesicle-reconstituted channels, while only moderately (14%) affected by the intact mucus glycoprotein, was found significantly inhibited (59%) by the acidic glycoprotein fraction. This effect was associated with the sialic acid and sulfate ester groups of the glycoprotein, as their removal caused a loss in the inhibition.
2.
2. The channel complex in the presence of epidermal growth factor (EGF) and ATP responded by an increase in protein tyrosine phosphorylation of 55 and 170 kDa proteins, and the vesicles containing the phosphorylated channels showed a 50% increase in
45Ca
2+ uptake. The phosphorylation and the calcium uptake were susceptible to inhibition by a specific tyrosine kinase inhibitor, genistein.
3.
3. The channel protein phosphorylation was inhibited by the acidic mucus glycoprotein, which also interfered with the binding of EGF to the channel protein. The inhibitory effect was dependent upon the presence of sulfate ester and sialic acid groups, as evidenced by the loss of the glycoprotein inhibitory capacity following their removal.
4.
4. The results suggest that the acidic gastric mucus glycoproteins, by modulating the EGF-controlled calcium channel phosphorylation, play a major role in gastric mucosal calcium homeostasis.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(93)90242-7</identifier><identifier>PMID: 8393811</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Adenosine Triphosphate - metabolism ; Adenosine Triphosphate - pharmacology ; Animals ; Binding Sites ; Biological and medical sciences ; Calcium - metabolism ; Calcium Channel Blockers - pharmacology ; Calcium Channels - drug effects ; Calcium Channels - metabolism ; Cell physiology ; Epidermal Growth Factor - metabolism ; Epidermal Growth Factor - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gastric Mucosa - metabolism ; Genistein ; Glycoproteins - pharmacology ; Humans ; In Vitro Techniques ; Isoflavones - pharmacology ; Male ; Membrane and intracellular transports ; Molecular and cellular biology ; Mucus - metabolism ; Phosphorylation ; Protein-Tyrosine Kinases - antagonists & inhibitors ; Rats ; Rats, Sprague-Dawley ; Tyrosine - metabolism</subject><ispartof>International journal of biochemistry, 1993-06, Vol.25 (6), p.869-878</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-c4b58ecba7013c9260b023778032b8ceec9607ef50f84abeb4e4bc0d9f4cd42e3</citedby><cites>FETCH-LOGICAL-c452t-c4b58ecba7013c9260b023778032b8ceec9607ef50f84abeb4e4bc0d9f4cd42e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4857808$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8393811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Slomiany, B.L.</creatorcontrib><creatorcontrib>Murty, V.L.N.</creatorcontrib><creatorcontrib>Liu, J.</creatorcontrib><creatorcontrib>Piotrowski, J.</creatorcontrib><creatorcontrib>Czajkowski, A.</creatorcontrib><creatorcontrib>Slomiany, A.</creatorcontrib><title>Modulation of gastric mucosal calcium channel activity by mucus glycoprotein</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. The effect of gastric mucus glycoprotein on the activity of calcium channel isolated from gastric epithelial cell membrane was investigated. The
45Ca
2+ uptake into the vesicle-reconstituted channels, while only moderately (14%) affected by the intact mucus glycoprotein, was found significantly inhibited (59%) by the acidic glycoprotein fraction. This effect was associated with the sialic acid and sulfate ester groups of the glycoprotein, as their removal caused a loss in the inhibition.
2.
2. The channel complex in the presence of epidermal growth factor (EGF) and ATP responded by an increase in protein tyrosine phosphorylation of 55 and 170 kDa proteins, and the vesicles containing the phosphorylated channels showed a 50% increase in
45Ca
2+ uptake. The phosphorylation and the calcium uptake were susceptible to inhibition by a specific tyrosine kinase inhibitor, genistein.
3.
3. The channel protein phosphorylation was inhibited by the acidic mucus glycoprotein, which also interfered with the binding of EGF to the channel protein. The inhibitory effect was dependent upon the presence of sulfate ester and sialic acid groups, as evidenced by the loss of the glycoprotein inhibitory capacity following their removal.
4.
4. The results suggest that the acidic gastric mucus glycoproteins, by modulating the EGF-controlled calcium channel phosphorylation, play a major role in gastric mucosal calcium homeostasis.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - metabolism</subject><subject>Cell physiology</subject><subject>Epidermal Growth Factor - metabolism</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastric Mucosa - metabolism</subject><subject>Genistein</subject><subject>Glycoproteins - pharmacology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Isoflavones - pharmacology</subject><subject>Male</subject><subject>Membrane and intracellular transports</subject><subject>Molecular and cellular biology</subject><subject>Mucus - metabolism</subject><subject>Phosphorylation</subject><subject>Protein-Tyrosine Kinases - antagonists & inhibitors</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Tyrosine - metabolism</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQgHNQfKz-A4UeRPSwmjTpJrkIsviCFS8K3kIyna6RttGkFfbf27rLHr1kIPPN6yPkhNErRtnsmtKcTiVj7xeaX2qai3wqd8jB9nufHKb0SSnTSrA9sqe45oqxA7J4DmVf286HNgtVtrSpix6ypoeQbJ2BrcH3TQYftm2xzix0_sd3q8ytRqZP2bJeQfiKoUPfHpHdytYJjzdxQt7u717nj9PFy8PT_HYxBVHk3fC6QiE4KynjoPMZdTTnUirKc6cAEfSMSqwKWilhHTqBwgEtdSWgFDnyCTlf9x3mfveYOtP4BFjXtsXQJyMLpbnUagDFGoQYUopYma_oGxtXhlEzijOjITMaMpqbP3FGDmWnm_69a7DcFm2sDfmzTd6mwVAVbQs-bTGhiuGWcfrNGsPBxY_HaBJ4bAFLHxE6Uwb__x6_Ey2NLA</recordid><startdate>19930601</startdate><enddate>19930601</enddate><creator>Slomiany, B.L.</creator><creator>Murty, V.L.N.</creator><creator>Liu, J.</creator><creator>Piotrowski, J.</creator><creator>Czajkowski, A.</creator><creator>Slomiany, A.</creator><general>Elsevier B.V</general><general>Pergamon</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930601</creationdate><title>Modulation of gastric mucosal calcium channel activity by mucus glycoprotein</title><author>Slomiany, B.L. ; Murty, V.L.N. ; Liu, J. ; Piotrowski, J. ; Czajkowski, A. ; Slomiany, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-c4b58ecba7013c9260b023778032b8ceec9607ef50f84abeb4e4bc0d9f4cd42e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - metabolism</topic><topic>Cell physiology</topic><topic>Epidermal Growth Factor - metabolism</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastric Mucosa - metabolism</topic><topic>Genistein</topic><topic>Glycoproteins - pharmacology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Isoflavones - pharmacology</topic><topic>Male</topic><topic>Membrane and intracellular transports</topic><topic>Molecular and cellular biology</topic><topic>Mucus - metabolism</topic><topic>Phosphorylation</topic><topic>Protein-Tyrosine Kinases - antagonists & inhibitors</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Tyrosine - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Slomiany, B.L.</creatorcontrib><creatorcontrib>Murty, V.L.N.</creatorcontrib><creatorcontrib>Liu, J.</creatorcontrib><creatorcontrib>Piotrowski, J.</creatorcontrib><creatorcontrib>Czajkowski, A.</creatorcontrib><creatorcontrib>Slomiany, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slomiany, B.L.</au><au>Murty, V.L.N.</au><au>Liu, J.</au><au>Piotrowski, J.</au><au>Czajkowski, A.</au><au>Slomiany, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of gastric mucosal calcium channel activity by mucus glycoprotein</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1993-06-01</date><risdate>1993</risdate><volume>25</volume><issue>6</issue><spage>869</spage><epage>878</epage><pages>869-878</pages><issn>0020-711X</issn><abstract>1.
1. The effect of gastric mucus glycoprotein on the activity of calcium channel isolated from gastric epithelial cell membrane was investigated. The
45Ca
2+ uptake into the vesicle-reconstituted channels, while only moderately (14%) affected by the intact mucus glycoprotein, was found significantly inhibited (59%) by the acidic glycoprotein fraction. This effect was associated with the sialic acid and sulfate ester groups of the glycoprotein, as their removal caused a loss in the inhibition.
2.
2. The channel complex in the presence of epidermal growth factor (EGF) and ATP responded by an increase in protein tyrosine phosphorylation of 55 and 170 kDa proteins, and the vesicles containing the phosphorylated channels showed a 50% increase in
45Ca
2+ uptake. The phosphorylation and the calcium uptake were susceptible to inhibition by a specific tyrosine kinase inhibitor, genistein.
3.
3. The channel protein phosphorylation was inhibited by the acidic mucus glycoprotein, which also interfered with the binding of EGF to the channel protein. The inhibitory effect was dependent upon the presence of sulfate ester and sialic acid groups, as evidenced by the loss of the glycoprotein inhibitory capacity following their removal.
4.
4. The results suggest that the acidic gastric mucus glycoproteins, by modulating the EGF-controlled calcium channel phosphorylation, play a major role in gastric mucosal calcium homeostasis.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>8393811</pmid><doi>10.1016/0020-711X(93)90242-7</doi><tpages>10</tpages></addata></record> |
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| subjects | Adenosine Triphosphate - metabolism Adenosine Triphosphate - pharmacology Animals Binding Sites Biological and medical sciences Calcium - metabolism Calcium Channel Blockers - pharmacology Calcium Channels - drug effects Calcium Channels - metabolism Cell physiology Epidermal Growth Factor - metabolism Epidermal Growth Factor - pharmacology Fundamental and applied biological sciences. Psychology Gastric Mucosa - metabolism Genistein Glycoproteins - pharmacology Humans In Vitro Techniques Isoflavones - pharmacology Male Membrane and intracellular transports Molecular and cellular biology Mucus - metabolism Phosphorylation Protein-Tyrosine Kinases - antagonists & inhibitors Rats Rats, Sprague-Dawley Tyrosine - metabolism |
| title | Modulation of gastric mucosal calcium channel activity by mucus glycoprotein |
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