High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk
A simplified and accurate method for determination of naturally occurring vitamin D3 in bovine milk was established by highperformance liquid chromatography (HPLC) using successively reversedphase and straight-phase columns. Exactly 25.0 ml of a sample of bovine milk was taken and the lipid was extr...
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Veröffentlicht in: | Journal of Nutritional Science and Vitaminology 1984, Vol.30(5), pp.431-439 |
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creator | Okano, T. (Kobe Women's Coll. of Pharmacy (Japan)) Yokoshima, K Kobayashi, T |
description | A simplified and accurate method for determination of naturally occurring vitamin D3 in bovine milk was established by highperformance liquid chromatography (HPLC) using successively reversedphase and straight-phase columns. Exactly 25.0 ml of a sample of bovine milk was taken and the lipid was extracted with a solvent mixture of petroleum ether and ethyl ether (1:1) with small amounts of ethanol and Triton X-100, present. The extracted lipid was subjected to the first preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (1:1) as the mobile phase, and a fraction containing vitamin D3 was isolated. The fraction was subsequently subjected to the second analytical HPLC using a Zorbax SIL column (straight-phase type) with 0.4% isopropanol in n-hexane as the mobile phase. Vitamin D3 was assayed by estimating the peak height on the chromatogram. The overall recovery and CV values were 92.1±8.7% and 9.4%, respectively, which were satisfactory. The proposed method was applied to several kinds of colostrum, early and later bovine milk in pairs. The assayed values in the colostrum of the group with large amounts of vitamin D3 administered before delivery to prevent parturient paresis were higher than those in the group with no administration. However, the values of the former group generally decreased in the respective early and later milk in step and there were few differences among those in the later milk of the two groups. The assayed values in later milk were 30-80 IU/liter. |
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(Kobe Women's Coll. of Pharmacy (Japan)) ; Yokoshima, K ; Kobayashi, T</creator><creatorcontrib>Okano, T. (Kobe Women's Coll. of Pharmacy (Japan)) ; Yokoshima, K ; Kobayashi, T</creatorcontrib><description>A simplified and accurate method for determination of naturally occurring vitamin D3 in bovine milk was established by highperformance liquid chromatography (HPLC) using successively reversedphase and straight-phase columns. Exactly 25.0 ml of a sample of bovine milk was taken and the lipid was extracted with a solvent mixture of petroleum ether and ethyl ether (1:1) with small amounts of ethanol and Triton X-100, present. The extracted lipid was subjected to the first preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (1:1) as the mobile phase, and a fraction containing vitamin D3 was isolated. The fraction was subsequently subjected to the second analytical HPLC using a Zorbax SIL column (straight-phase type) with 0.4% isopropanol in n-hexane as the mobile phase. Vitamin D3 was assayed by estimating the peak height on the chromatogram. The overall recovery and CV values were 92.1±8.7% and 9.4%, respectively, which were satisfactory. The proposed method was applied to several kinds of colostrum, early and later bovine milk in pairs. The assayed values in the colostrum of the group with large amounts of vitamin D3 administered before delivery to prevent parturient paresis were higher than those in the group with no administration. However, the values of the former group generally decreased in the respective early and later milk in step and there were few differences among those in the later milk of the two groups. The assayed values in later milk were 30-80 IU/liter.</description><identifier>ISSN: 0301-4800</identifier><identifier>EISSN: 1881-7742</identifier><identifier>DOI: 10.3177/jnsv.30.431</identifier><identifier>PMID: 6098636</identifier><language>eng</language><publisher>Japan: Center for Academic Publications Japan</publisher><subject>ANALYTICAL METHODS ; Animals ; BOVIN ; bovine milk ; CALOSTRO ; CATTLE ; Cholecalciferol - analysis ; CHROMATOGRAPHIE LIQUIDE HAUTE PRESS ; Chromatography, High Pressure Liquid - methods ; COLOSTRUM ; Colostrum - analysis ; cow's milk ; CROMATOGRAFIA LIQUIDA ALTA PRESION ; determination of vitamin D3 ; early milk ; GANADO BOVINO ; HIGH PRESSURE LIQUID CHROMATOGRAPHY ; high-performance liquid chromatography ; LAIT ; later milk ; LECHE ; MILK ; Milk - analysis ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; Time Factors ; VITAMIN D ; VITAMINA D ; VITAMINE D</subject><ispartof>Journal of Nutritional Science and Vitaminology, 1984, Vol.30(5), pp.431-439</ispartof><rights>the Center for Academic Publications Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-36db6aa433838bec81aa8044527a564277363d1fca61e7e2d72ec0b16c9888853</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6098636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okano, T. (Kobe Women's Coll. of Pharmacy (Japan))</creatorcontrib><creatorcontrib>Yokoshima, K</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><title>High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk</title><title>Journal of Nutritional Science and Vitaminology</title><addtitle>J Nutr Sci Vitaminol</addtitle><description>A simplified and accurate method for determination of naturally occurring vitamin D3 in bovine milk was established by highperformance liquid chromatography (HPLC) using successively reversedphase and straight-phase columns. Exactly 25.0 ml of a sample of bovine milk was taken and the lipid was extracted with a solvent mixture of petroleum ether and ethyl ether (1:1) with small amounts of ethanol and Triton X-100, present. The extracted lipid was subjected to the first preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (1:1) as the mobile phase, and a fraction containing vitamin D3 was isolated. The fraction was subsequently subjected to the second analytical HPLC using a Zorbax SIL column (straight-phase type) with 0.4% isopropanol in n-hexane as the mobile phase. Vitamin D3 was assayed by estimating the peak height on the chromatogram. The overall recovery and CV values were 92.1±8.7% and 9.4%, respectively, which were satisfactory. The proposed method was applied to several kinds of colostrum, early and later bovine milk in pairs. The assayed values in the colostrum of the group with large amounts of vitamin D3 administered before delivery to prevent parturient paresis were higher than those in the group with no administration. However, the values of the former group generally decreased in the respective early and later milk in step and there were few differences among those in the later milk of the two groups. The assayed values in later milk were 30-80 IU/liter.</description><subject>ANALYTICAL METHODS</subject><subject>Animals</subject><subject>BOVIN</subject><subject>bovine milk</subject><subject>CALOSTRO</subject><subject>CATTLE</subject><subject>Cholecalciferol - analysis</subject><subject>CHROMATOGRAPHIE LIQUIDE HAUTE PRESS</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>COLOSTRUM</subject><subject>Colostrum - analysis</subject><subject>cow's milk</subject><subject>CROMATOGRAFIA LIQUIDA ALTA PRESION</subject><subject>determination of vitamin D3</subject><subject>early milk</subject><subject>GANADO BOVINO</subject><subject>HIGH PRESSURE LIQUID CHROMATOGRAPHY</subject><subject>high-performance liquid chromatography</subject><subject>LAIT</subject><subject>later milk</subject><subject>LECHE</subject><subject>MILK</subject><subject>Milk - analysis</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>Time Factors</subject><subject>VITAMIN D</subject><subject>VITAMINA D</subject><subject>VITAMINE D</subject><issn>0301-4800</issn><issn>1881-7742</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEFv1DAQhS1EVZaWEzckJJ-4QBY7dmznWLVAqSrBoZytiTPZ9ZLEWztZqf8eL1ntHN5o9N58h0fIe87Wgmv9dTemw1qwtRT8FVlxY3ihtSxfkxUTjBfSMPaGvE1px5isjTSX5FKx2iihVmS495ttscfYhTjA6JD2_nn2LXXbGAaYwibCfusdbXHCOPgRJh9GGjp68BPkm94JmrUJBz8idaEPaYrz8IUixP6FwtjSHvIrHXz_95pcdNAnfHfaV-TP929Pt_fF468fP29vHgtXlXIqhGobBSCFMMI06AwHMEzKqtRQKVlqLZRoeedAcdRYtrpExxquXG3yVOKKfFq4-xieZ0yTHXxy2PcwYpiT1ZUxqipVDn5egi6GlCJ2dh_9APHFcmaP5dpjuVYwm8vN6Y8n7NwM2J6zpzazf7f4uzTBBs8-xMm7Hv-zeK3FkVctkrFn220hWhwz5sOC6SBY2ESf7MPvWjChZC3-AYtzlxc</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Okano, T. (Kobe Women's Coll. of Pharmacy (Japan))</creator><creator>Yokoshima, K</creator><creator>Kobayashi, T</creator><general>Center for Academic Publications Japan</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk</title><author>Okano, T. (Kobe Women's Coll. of Pharmacy (Japan)) ; Yokoshima, K ; Kobayashi, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-36db6aa433838bec81aa8044527a564277363d1fca61e7e2d72ec0b16c9888853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>ANALYTICAL METHODS</topic><topic>Animals</topic><topic>BOVIN</topic><topic>bovine milk</topic><topic>CALOSTRO</topic><topic>CATTLE</topic><topic>Cholecalciferol - analysis</topic><topic>CHROMATOGRAPHIE LIQUIDE HAUTE PRESS</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>COLOSTRUM</topic><topic>Colostrum - analysis</topic><topic>cow's milk</topic><topic>CROMATOGRAFIA LIQUIDA ALTA PRESION</topic><topic>determination of vitamin D3</topic><topic>early milk</topic><topic>GANADO BOVINO</topic><topic>HIGH PRESSURE LIQUID CHROMATOGRAPHY</topic><topic>high-performance liquid chromatography</topic><topic>LAIT</topic><topic>later milk</topic><topic>LECHE</topic><topic>MILK</topic><topic>Milk - analysis</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>Time Factors</topic><topic>VITAMIN D</topic><topic>VITAMINA D</topic><topic>VITAMINE D</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okano, T. (Kobe Women's Coll. of Pharmacy (Japan))</creatorcontrib><creatorcontrib>Yokoshima, K</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Nutritional Science and Vitaminology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okano, T. (Kobe Women's Coll. of Pharmacy (Japan))</au><au>Yokoshima, K</au><au>Kobayashi, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk</atitle><jtitle>Journal of Nutritional Science and Vitaminology</jtitle><addtitle>J Nutr Sci Vitaminol</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>30</volume><issue>5</issue><spage>431</spage><epage>439</epage><pages>431-439</pages><issn>0301-4800</issn><eissn>1881-7742</eissn><abstract>A simplified and accurate method for determination of naturally occurring vitamin D3 in bovine milk was established by highperformance liquid chromatography (HPLC) using successively reversedphase and straight-phase columns. Exactly 25.0 ml of a sample of bovine milk was taken and the lipid was extracted with a solvent mixture of petroleum ether and ethyl ether (1:1) with small amounts of ethanol and Triton X-100, present. The extracted lipid was subjected to the first preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (1:1) as the mobile phase, and a fraction containing vitamin D3 was isolated. The fraction was subsequently subjected to the second analytical HPLC using a Zorbax SIL column (straight-phase type) with 0.4% isopropanol in n-hexane as the mobile phase. Vitamin D3 was assayed by estimating the peak height on the chromatogram. The overall recovery and CV values were 92.1±8.7% and 9.4%, respectively, which were satisfactory. The proposed method was applied to several kinds of colostrum, early and later bovine milk in pairs. The assayed values in the colostrum of the group with large amounts of vitamin D3 administered before delivery to prevent parturient paresis were higher than those in the group with no administration. However, the values of the former group generally decreased in the respective early and later milk in step and there were few differences among those in the later milk of the two groups. The assayed values in later milk were 30-80 IU/liter.</abstract><cop>Japan</cop><pub>Center for Academic Publications Japan</pub><pmid>6098636</pmid><doi>10.3177/jnsv.30.431</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | ANALYTICAL METHODS Animals BOVIN bovine milk CALOSTRO CATTLE Cholecalciferol - analysis CHROMATOGRAPHIE LIQUIDE HAUTE PRESS Chromatography, High Pressure Liquid - methods COLOSTRUM Colostrum - analysis cow's milk CROMATOGRAFIA LIQUIDA ALTA PRESION determination of vitamin D3 early milk GANADO BOVINO HIGH PRESSURE LIQUID CHROMATOGRAPHY high-performance liquid chromatography LAIT later milk LECHE MILK Milk - analysis TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS Time Factors VITAMIN D VITAMINA D VITAMINE D |
title | High-performance liquid chromatographic determination of vitamin D3 in bovine colostrum, early and later milk |
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