Molecular Identification of Onchocerciasis Vector Sibling Species in Black Flies (Diptera: Simuliidae)
A number of sibling species in the Simulium damnosum species complex transmit River Blindness (onchocerciasis) in tropical Africa. Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially dis...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-07, Vol.194 (2), p.628-634 |
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| creator | Brockhouse, C.L. Vajime, C.G. Marin, R. Tanguay, R.M. |
| description | A number of sibling species in the Simulium damnosum species complex transmit River Blindness (onchocerciasis) in tropical Africa. Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially disease-transmitting adult females to a particular species is virtually impossible. Two molecular assays to identify the sibling species of biting female flies are presented. The first relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females. Both assays yield sibling-specific size markers that can be used to identify the sibling species of biting adult females. To illustrate the use of these assays, a molecular characterization is given for two S. damnosum siblings (squamosum, sirbanum) collected from cytologically verified populations, and a partial characterization of a third sibling (damnosum s. str.) found as a contaminant in these samples. |
| doi_str_mv | 10.1006/bbrc.1993.1867 |
| format | Article |
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Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially disease-transmitting adult females to a particular species is virtually impossible. Two molecular assays to identify the sibling species of biting female flies are presented. The first relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females. Both assays yield sibling-specific size markers that can be used to identify the sibling species of biting adult females. To illustrate the use of these assays, a molecular characterization is given for two S. damnosum siblings (squamosum, sirbanum) collected from cytologically verified populations, and a partial characterization of a third sibling (damnosum s. str.) found as a contaminant in these samples.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1867</identifier><identifier>PMID: 8343149</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>adn ; adulte ; adultos ; adults ; Animals ; biochemistry ; biochimie ; Biological and medical sciences ; bioquimica ; Chromatin. Chromosome ; Chromosomes - physiology ; Diptera ; dna ; DNA - genetics ; DNA - isolation & purification ; DNA, Ribosomal - genetics ; espece ; especies ; Female ; females ; femelle ; fratrie ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; hembra ; hermanos ; Humans ; identificacion ; identification ; Immunohistochemistry ; immunologie ; immunology ; inmunologia ; Insect Vectors - classification ; Insect Vectors - genetics ; Larva ; larvae ; larvas ; larve ; Molecular and cellular biology ; Molecular genetics ; Oligodeoxyribonucleotides ; Onchocerciasis, Ocular - transmission ; Polymerase Chain Reaction - methods ; proteinas ; proteine ; proteins ; ribosomas ; ribosome ; ribosomes ; RNA, Ribosomal - genetics ; Salivary Glands - cytology ; siblings ; Simuliidae ; Simuliidae - classification ; Simuliidae - genetics ; Simulium damnosum ; species ; Transcription, Genetic ; vecteur de maladie ; vectores ; vectors</subject><ispartof>Biochemical and biophysical research communications, 1993-07, Vol.194 (2), p.628-634</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-de29cca25440e8d11d71fcabf68098a37cecbbf21ab7510c708f6a47e9eb4e023</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X8371867X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4834335$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8343149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brockhouse, C.L.</creatorcontrib><creatorcontrib>Vajime, C.G.</creatorcontrib><creatorcontrib>Marin, R.</creatorcontrib><creatorcontrib>Tanguay, R.M.</creatorcontrib><creatorcontrib>Razi Institute, Karaj (Iran Islamic Republic)</creatorcontrib><title>Molecular Identification of Onchocerciasis Vector Sibling Species in Black Flies (Diptera: Simuliidae)</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>A number of sibling species in the Simulium damnosum species complex transmit River Blindness (onchocerciasis) in tropical Africa. Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially disease-transmitting adult females to a particular species is virtually impossible. Two molecular assays to identify the sibling species of biting female flies are presented. The first relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females. Both assays yield sibling-specific size markers that can be used to identify the sibling species of biting adult females. To illustrate the use of these assays, a molecular characterization is given for two S. damnosum siblings (squamosum, sirbanum) collected from cytologically verified populations, and a partial characterization of a third sibling (damnosum s. str.) found as a contaminant in these samples.</description><subject>adn</subject><subject>adulte</subject><subject>adultos</subject><subject>adults</subject><subject>Animals</subject><subject>biochemistry</subject><subject>biochimie</subject><subject>Biological and medical sciences</subject><subject>bioquimica</subject><subject>Chromatin. Chromosome</subject><subject>Chromosomes - physiology</subject><subject>Diptera</subject><subject>dna</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA, Ribosomal - genetics</subject><subject>espece</subject><subject>especies</subject><subject>Female</subject><subject>females</subject><subject>femelle</subject><subject>fratrie</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>hembra</subject><subject>hermanos</subject><subject>Humans</subject><subject>identificacion</subject><subject>identification</subject><subject>Immunohistochemistry</subject><subject>immunologie</subject><subject>immunology</subject><subject>inmunologia</subject><subject>Insect Vectors - classification</subject><subject>Insect Vectors - genetics</subject><subject>Larva</subject><subject>larvae</subject><subject>larvas</subject><subject>larve</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Oligodeoxyribonucleotides</subject><subject>Onchocerciasis, Ocular - transmission</subject><subject>Polymerase Chain Reaction - methods</subject><subject>proteinas</subject><subject>proteine</subject><subject>proteins</subject><subject>ribosomas</subject><subject>ribosome</subject><subject>ribosomes</subject><subject>RNA, Ribosomal - genetics</subject><subject>Salivary Glands - cytology</subject><subject>siblings</subject><subject>Simuliidae</subject><subject>Simuliidae - classification</subject><subject>Simuliidae - genetics</subject><subject>Simulium damnosum</subject><subject>species</subject><subject>Transcription, Genetic</subject><subject>vecteur de maladie</subject><subject>vectores</subject><subject>vectors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UFvFCEUB3BiNHWtXr1p5mCMHnblMcwM9KbVapOaHtYab4R586goO6wwY-K3l8luejOGAyHvx4O8P2NPgW-A8_ZN3yfcgNb1BlTb3WMr4JqvBXB5n614EWuh4dtD9ijnH5wDyFafsBNVyxqkXjH3OQbCOdhUXQ40Tt55tJOPYxVddT3i94iU0Nvsc_WVcIqp2vo--PG22u4JPeXKj9W7YPFndRGW46v3fj9RsmcF7ubg_WDp9WP2wNmQ6clxP2U3Fx--nH9aX11_vDx_e7VGKcS0HkhoRCsaKTmpAWDowKHtXau4VrbukLDvnQDbdw1w7LhyrZUdaeolcVGfspeHvvsUf82UJ7PzGSkEO1Kcs-kapaSC_0NoO9GUVeDmADHFnBM5s09-Z9MfA9wsCZglAbMkYJYEyoVnx85zv6Phjh9HXuovjnWb0QaX7Ig-3zG5uLop7PmBORuNvU2F3GzLK5KDAN0sQB0AlXn-9pRMLnGMSINPJSgzRP-vL_4FOvSrPA</recordid><startdate>19930730</startdate><enddate>19930730</enddate><creator>Brockhouse, C.L.</creator><creator>Vajime, C.G.</creator><creator>Marin, R.</creator><creator>Tanguay, R.M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930730</creationdate><title>Molecular Identification of Onchocerciasis Vector Sibling Species in Black Flies (Diptera: Simuliidae)</title><author>Brockhouse, C.L. ; Vajime, C.G. ; Marin, R. ; Tanguay, R.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-de29cca25440e8d11d71fcabf68098a37cecbbf21ab7510c708f6a47e9eb4e023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>adn</topic><topic>adulte</topic><topic>adultos</topic><topic>adults</topic><topic>Animals</topic><topic>biochemistry</topic><topic>biochimie</topic><topic>Biological and medical sciences</topic><topic>bioquimica</topic><topic>Chromatin. Chromosome</topic><topic>Chromosomes - physiology</topic><topic>Diptera</topic><topic>dna</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA, Ribosomal - genetics</topic><topic>espece</topic><topic>especies</topic><topic>Female</topic><topic>females</topic><topic>femelle</topic><topic>fratrie</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>hembra</topic><topic>hermanos</topic><topic>Humans</topic><topic>identificacion</topic><topic>identification</topic><topic>Immunohistochemistry</topic><topic>immunologie</topic><topic>immunology</topic><topic>inmunologia</topic><topic>Insect Vectors - classification</topic><topic>Insect Vectors - genetics</topic><topic>Larva</topic><topic>larvae</topic><topic>larvas</topic><topic>larve</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Oligodeoxyribonucleotides</topic><topic>Onchocerciasis, Ocular - transmission</topic><topic>Polymerase Chain Reaction - methods</topic><topic>proteinas</topic><topic>proteine</topic><topic>proteins</topic><topic>ribosomas</topic><topic>ribosome</topic><topic>ribosomes</topic><topic>RNA, Ribosomal - genetics</topic><topic>Salivary Glands - cytology</topic><topic>siblings</topic><topic>Simuliidae</topic><topic>Simuliidae - classification</topic><topic>Simuliidae - genetics</topic><topic>Simulium damnosum</topic><topic>species</topic><topic>Transcription, Genetic</topic><topic>vecteur de maladie</topic><topic>vectores</topic><topic>vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brockhouse, C.L.</creatorcontrib><creatorcontrib>Vajime, C.G.</creatorcontrib><creatorcontrib>Marin, R.</creatorcontrib><creatorcontrib>Tanguay, R.M.</creatorcontrib><creatorcontrib>Razi Institute, Karaj (Iran Islamic Republic)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brockhouse, C.L.</au><au>Vajime, C.G.</au><au>Marin, R.</au><au>Tanguay, R.M.</au><aucorp>Razi Institute, Karaj (Iran Islamic Republic)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Identification of Onchocerciasis Vector Sibling Species in Black Flies (Diptera: Simuliidae)</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-07-30</date><risdate>1993</risdate><volume>194</volume><issue>2</issue><spage>628</spage><epage>634</epage><pages>628-634</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>A number of sibling species in the Simulium damnosum species complex transmit River Blindness (onchocerciasis) in tropical Africa. Black fly sibling species can be reliably recognized only through analysis of the larval polytene chromosomes. The routine, unambiguous assignment of the potentially disease-transmitting adult females to a particular species is virtually impossible. Two molecular assays to identify the sibling species of biting female flies are presented. The first relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females. Both assays yield sibling-specific size markers that can be used to identify the sibling species of biting adult females. To illustrate the use of these assays, a molecular characterization is given for two S. damnosum siblings (squamosum, sirbanum) collected from cytologically verified populations, and a partial characterization of a third sibling (damnosum s. str.) found as a contaminant in these samples.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8343149</pmid><doi>10.1006/bbrc.1993.1867</doi><tpages>7</tpages></addata></record> |
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| subjects | adn adulte adultos adults Animals biochemistry biochimie Biological and medical sciences bioquimica Chromatin. Chromosome Chromosomes - physiology Diptera dna DNA - genetics DNA - isolation & purification DNA, Ribosomal - genetics espece especies Female females femelle fratrie Fundamental and applied biological sciences. Psychology Gene Expression hembra hermanos Humans identificacion identification Immunohistochemistry immunologie immunology inmunologia Insect Vectors - classification Insect Vectors - genetics Larva larvae larvas larve Molecular and cellular biology Molecular genetics Oligodeoxyribonucleotides Onchocerciasis, Ocular - transmission Polymerase Chain Reaction - methods proteinas proteine proteins ribosomas ribosome ribosomes RNA, Ribosomal - genetics Salivary Glands - cytology siblings Simuliidae Simuliidae - classification Simuliidae - genetics Simulium damnosum species Transcription, Genetic vecteur de maladie vectores vectors |
| title | Molecular Identification of Onchocerciasis Vector Sibling Species in Black Flies (Diptera: Simuliidae) |
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