Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces spp
The whiE gene cluster of Streptomyces coelicolor, which is related to gene sets encoding the biosynthesis of polycyclic aromatic polyketide antibiotics, determines a spore pigment. Southern blotting using probes from three different parts of the whiE cluster revealed related gene sets in about half...
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| Veröffentlicht in: | Gene 1993-08, Vol.130 (1), p.107-116 |
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| creator | Blanco, G. Brianb, P. Pereda, A. Méndez, C. Salas, J.A. Chater, K.F. |
| description | The
whiE gene cluster of
Streptomyces coelicolor, which is related to gene sets encoding the biosynthesis of polycyclic aromatic polyketide antibiotics, determines a spore pigment. Southern blotting using probes from three different parts of the
whiE cluster revealed related gene sets in about half of a collection of diverse
Streptomyces strains. A 5.2-kb segment of one such cluster,
sch, previously shown to determine spore pigmentation in
Streptomyces halstedii, was sequenced. Seven open reading frames (ORFs), two of them incomplete, were found. Six of the ORFs resemble the known part of the
whiE cluster closely. The derived gene products include a ketosynthase (= condensing enzyme) pair, acyl carrier protein and cyclase, as well as two of unidentified function. The seventh ORF diverges from the main cluster and encodes a protein that resembles a dichlorophenol hydroxylase. Comparison with sequences of related gene sets for the biosynthesis of antibiotics suggests that gene clusters destined to specify pigment production diverged from those destined to specify antibiotics early in the evolution of the
Streptomyces genus. |
| doi_str_mv | 10.1016/0378-1119(93)90352-4 |
| format | Article |
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whiE gene cluster of
Streptomyces coelicolor, which is related to gene sets encoding the biosynthesis of polycyclic aromatic polyketide antibiotics, determines a spore pigment. Southern blotting using probes from three different parts of the
whiE cluster revealed related gene sets in about half of a collection of diverse
Streptomyces strains. A 5.2-kb segment of one such cluster,
sch, previously shown to determine spore pigmentation in
Streptomyces halstedii, was sequenced. Seven open reading frames (ORFs), two of them incomplete, were found. Six of the ORFs resemble the known part of the
whiE cluster closely. The derived gene products include a ketosynthase (= condensing enzyme) pair, acyl carrier protein and cyclase, as well as two of unidentified function. The seventh ORF diverges from the main cluster and encodes a protein that resembles a dichlorophenol hydroxylase. Comparison with sequences of related gene sets for the biosynthesis of antibiotics suggests that gene clusters destined to specify pigment production diverged from those destined to specify antibiotics early in the evolution of the
Streptomyces genus.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(93)90352-4</identifier><identifier>PMID: 8344517</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Anti-Bacterial Agents - biosynthesis ; aromatic hydroxylase ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; beta-Glucosidase - genetics ; Biological and medical sciences ; Biological Evolution ; Blotting, Southern ; Carrier Proteins - genetics ; condensing enzyme ; Conserved Sequence ; DNA Probes ; DNA, Bacterial - analysis ; Fatty Acids - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetics ; ketoreductase ; Microbiology ; Molecular phylogeny ; Molecular Sequence Data ; Multienzyme Complexes - genetics ; Multigene Family ; Open Reading Frames ; Oxidoreductases - genetics ; Peptides ; Pigments, Biological - chemistry ; Pigments, Biological - genetics ; secondary metabolism ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Spores, Bacterial - chemistry ; sporulation ; Streptococcus - enzymology ; Streptococcus - genetics ; Streptomyces coelicolor A3 ; Streptomyces halstedii ; β-glucosidase</subject><ispartof>Gene, 1993-08, Vol.130 (1), p.107-116</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ac98d3fd60e899c1ed605d034870890b215c47c1886b25a9f7caa85b51bc519d3</citedby><cites>FETCH-LOGICAL-c386t-ac98d3fd60e899c1ed605d034870890b215c47c1886b25a9f7caa85b51bc519d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(93)90352-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4853166$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8344517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blanco, G.</creatorcontrib><creatorcontrib>Brianb, P.</creatorcontrib><creatorcontrib>Pereda, A.</creatorcontrib><creatorcontrib>Méndez, C.</creatorcontrib><creatorcontrib>Salas, J.A.</creatorcontrib><creatorcontrib>Chater, K.F.</creatorcontrib><title>Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces spp</title><title>Gene</title><addtitle>Gene</addtitle><description>The
whiE gene cluster of
Streptomyces coelicolor, which is related to gene sets encoding the biosynthesis of polycyclic aromatic polyketide antibiotics, determines a spore pigment. Southern blotting using probes from three different parts of the
whiE cluster revealed related gene sets in about half of a collection of diverse
Streptomyces strains. A 5.2-kb segment of one such cluster,
sch, previously shown to determine spore pigmentation in
Streptomyces halstedii, was sequenced. Seven open reading frames (ORFs), two of them incomplete, were found. Six of the ORFs resemble the known part of the
whiE cluster closely. The derived gene products include a ketosynthase (= condensing enzyme) pair, acyl carrier protein and cyclase, as well as two of unidentified function. The seventh ORF diverges from the main cluster and encodes a protein that resembles a dichlorophenol hydroxylase. Comparison with sequences of related gene sets for the biosynthesis of antibiotics suggests that gene clusters destined to specify pigment production diverged from those destined to specify antibiotics early in the evolution of the
Streptomyces genus.</description><subject>Amino Acid Sequence</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>aromatic hydroxylase</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>beta-Glucosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>Blotting, Southern</subject><subject>Carrier Proteins - genetics</subject><subject>condensing enzyme</subject><subject>Conserved Sequence</subject><subject>DNA Probes</subject><subject>DNA, Bacterial - analysis</subject><subject>Fatty Acids - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>ketoreductase</subject><subject>Microbiology</subject><subject>Molecular phylogeny</subject><subject>Molecular Sequence Data</subject><subject>Multienzyme Complexes - genetics</subject><subject>Multigene Family</subject><subject>Open Reading Frames</subject><subject>Oxidoreductases - genetics</subject><subject>Peptides</subject><subject>Pigments, Biological - chemistry</subject><subject>Pigments, Biological - genetics</subject><subject>secondary metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Spores, Bacterial - chemistry</subject><subject>sporulation</subject><subject>Streptococcus - enzymology</subject><subject>Streptococcus - genetics</subject><subject>Streptomyces coelicolor A3</subject><subject>Streptomyces halstedii</subject><subject>β-glucosidase</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcGOFCEQhonRrOPqG2jCwRg9tMIA3XAx2ay7rslGD-qZ0FDdoj1NC_Qk7Zv5dtIzkznKBVL_9xeV-hF6TslbSmj9jrBGVpRS9VqxN4owsa34A7ShslEVIUw-RJsz8hg9SeknKUeI7QW6kIxzQZsN-nu3tNE7_8dkH0ZsRoc_fL7CCX7PMFooBTMsCRJOc99DyqWAwcRhwbAPw7yaTFyw83uI_cEROhxhMBkcbn1Iy5h_QPYWFxVK35xwwYLzY4-nMCy_iujWf7IveAHTYYg0hQh48v0OxmLxI_6aI0w57Ba7TjNNT9GjzgwJnp3uS_T99ubb9V11_-Xjp-ur-8oyWefKWCUd61xNQCplKZSXcIRx2RCpSLulwvLGUinrdiuM6hprjBStoK0VVDl2iV4d-04xlKWkrHc-WRgGM0KYk26ElJzXrID8CNoYUorQ6Sn6XdmOpkSviek1Dr3GoRXTh8Q0L7YXp_5zuwN3Np0iKvrLk26SNUMXzWh9OmNcCkbrumDvjxiUXew9RJ2sXwNxPoLN2gX__zn-AWkPt20</recordid><startdate>19930816</startdate><enddate>19930816</enddate><creator>Blanco, G.</creator><creator>Brianb, P.</creator><creator>Pereda, A.</creator><creator>Méndez, C.</creator><creator>Salas, J.A.</creator><creator>Chater, K.F.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930816</creationdate><title>Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces spp</title><author>Blanco, G. ; Brianb, P. ; Pereda, A. ; Méndez, C. ; Salas, J.A. ; Chater, K.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ac98d3fd60e899c1ed605d034870890b215c47c1886b25a9f7caa85b51bc519d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>aromatic hydroxylase</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>beta-Glucosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>Blotting, Southern</topic><topic>Carrier Proteins - genetics</topic><topic>condensing enzyme</topic><topic>Conserved Sequence</topic><topic>DNA Probes</topic><topic>DNA, Bacterial - analysis</topic><topic>Fatty Acids - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>ketoreductase</topic><topic>Microbiology</topic><topic>Molecular phylogeny</topic><topic>Molecular Sequence Data</topic><topic>Multienzyme Complexes - genetics</topic><topic>Multigene Family</topic><topic>Open Reading Frames</topic><topic>Oxidoreductases - genetics</topic><topic>Peptides</topic><topic>Pigments, Biological - chemistry</topic><topic>Pigments, Biological - genetics</topic><topic>secondary metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Spores, Bacterial - chemistry</topic><topic>sporulation</topic><topic>Streptococcus - enzymology</topic><topic>Streptococcus - genetics</topic><topic>Streptomyces coelicolor A3</topic><topic>Streptomyces halstedii</topic><topic>β-glucosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blanco, G.</creatorcontrib><creatorcontrib>Brianb, P.</creatorcontrib><creatorcontrib>Pereda, A.</creatorcontrib><creatorcontrib>Méndez, C.</creatorcontrib><creatorcontrib>Salas, J.A.</creatorcontrib><creatorcontrib>Chater, K.F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blanco, G.</au><au>Brianb, P.</au><au>Pereda, A.</au><au>Méndez, C.</au><au>Salas, J.A.</au><au>Chater, K.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces spp</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1993-08-16</date><risdate>1993</risdate><volume>130</volume><issue>1</issue><spage>107</spage><epage>116</epage><pages>107-116</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The
whiE gene cluster of
Streptomyces coelicolor, which is related to gene sets encoding the biosynthesis of polycyclic aromatic polyketide antibiotics, determines a spore pigment. Southern blotting using probes from three different parts of the
whiE cluster revealed related gene sets in about half of a collection of diverse
Streptomyces strains. A 5.2-kb segment of one such cluster,
sch, previously shown to determine spore pigmentation in
Streptomyces halstedii, was sequenced. Seven open reading frames (ORFs), two of them incomplete, were found. Six of the ORFs resemble the known part of the
whiE cluster closely. The derived gene products include a ketosynthase (= condensing enzyme) pair, acyl carrier protein and cyclase, as well as two of unidentified function. The seventh ORF diverges from the main cluster and encodes a protein that resembles a dichlorophenol hydroxylase. Comparison with sequences of related gene sets for the biosynthesis of antibiotics suggests that gene clusters destined to specify pigment production diverged from those destined to specify antibiotics early in the evolution of the
Streptomyces genus.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8344517</pmid><doi>10.1016/0378-1119(93)90352-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1993-08, Vol.130 (1), p.107-116 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75884463 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence Anti-Bacterial Agents - biosynthesis aromatic hydroxylase Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacteriology Base Sequence beta-Glucosidase - genetics Biological and medical sciences Biological Evolution Blotting, Southern Carrier Proteins - genetics condensing enzyme Conserved Sequence DNA Probes DNA, Bacterial - analysis Fatty Acids - biosynthesis Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics ketoreductase Microbiology Molecular phylogeny Molecular Sequence Data Multienzyme Complexes - genetics Multigene Family Open Reading Frames Oxidoreductases - genetics Peptides Pigments, Biological - chemistry Pigments, Biological - genetics secondary metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Spores, Bacterial - chemistry sporulation Streptococcus - enzymology Streptococcus - genetics Streptomyces coelicolor A3 Streptomyces halstedii β-glucosidase |
| title | Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces spp |
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