Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter
CRP-cAMP was shown to activate transcription initiation at the Escherichia coli lac promoter in vitro as a result of two separate effects. An indirect component of the activation resulted from an enhancement of the fraction of promoters productively bound by RNA polymerase. This effect was due large...
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| Veröffentlicht in: | Journal of molecular biology 1984-12, Vol.180 (4), p.881-909 |
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| container_title | Journal of molecular biology |
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| creator | Malan, T.Philip Kolb, Annie Buc, Henri McClure, William R. |
| description | CRP-cAMP was shown to activate transcription initiation at the
Escherichia coli lac promoter
in vitro as a result of two separate effects. An indirect component of the activation resulted from an enhancement of the fraction of promoters productively bound by RNA polymerase. This effect was due largely to CRP-cAMP repression of RNA polymerase binding to an overlapping site (
lac P2) within the promoter region. In addition, a direct enhancement of RNA polymerase binding at the principal
lac promoter (
lac P1) was found. The combination of indirect and direct activation by CRP-cAMP was suggested to be responsible for the large activation observed
in vivo. Promoter strength parameters were also determined for the L8, UV5 and P
8 promoters. The effect of CRP-cAMP on these mutant promoters was shown to be consistent with the activation mechanism deduced for the
lac wild-type promoter. DNA supercoiling enhanced the promoter strength of the
lac wild-type and UV5 promoters. The combination of supercoiling and CRP-cAMP was necessary for optimal promoter strength for the
lac wild-type promoter. |
| doi_str_mv | 10.1016/0022-2836(84)90262-6 |
| format | Article |
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Escherichia coli lac promoter
in vitro as a result of two separate effects. An indirect component of the activation resulted from an enhancement of the fraction of promoters productively bound by RNA polymerase. This effect was due largely to CRP-cAMP repression of RNA polymerase binding to an overlapping site (
lac P2) within the promoter region. In addition, a direct enhancement of RNA polymerase binding at the principal
lac promoter (
lac P1) was found. The combination of indirect and direct activation by CRP-cAMP was suggested to be responsible for the large activation observed
in vivo. Promoter strength parameters were also determined for the L8, UV5 and P
8 promoters. The effect of CRP-cAMP on these mutant promoters was shown to be consistent with the activation mechanism deduced for the
lac wild-type promoter. DNA supercoiling enhanced the promoter strength of the
lac wild-type and UV5 promoters. The combination of supercoiling and CRP-cAMP was necessary for optimal promoter strength for the
lac wild-type promoter.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/0022-2836(84)90262-6</identifier><identifier>PMID: 6098691</identifier><identifier>CODEN: JMOBAK</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Applied sciences ; Cyclic AMP ; DNA, Superhelical ; DNA-Directed RNA Polymerases - metabolism ; Escherichia coli - genetics ; Exact sciences and technology ; Kinetics ; Lac Operon ; Models, Genetic ; Other techniques and industries ; Receptors, Cyclic AMP ; Templates, Genetic ; Transcription, Genetic</subject><ispartof>Journal of molecular biology, 1984-12, Vol.180 (4), p.881-909</ispartof><rights>1984</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-fe970f04cf1aaa88244e50deb35ffb9c2187a7f5d143222bf0e014f04cb8da6e3</citedby><cites>FETCH-LOGICAL-c386t-fe970f04cf1aaa88244e50deb35ffb9c2187a7f5d143222bf0e014f04cb8da6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-2836(84)90262-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8834775$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6098691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Malan, T.Philip</creatorcontrib><creatorcontrib>Kolb, Annie</creatorcontrib><creatorcontrib>Buc, Henri</creatorcontrib><creatorcontrib>McClure, William R.</creatorcontrib><title>Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>CRP-cAMP was shown to activate transcription initiation at the
Escherichia coli lac promoter
in vitro as a result of two separate effects. An indirect component of the activation resulted from an enhancement of the fraction of promoters productively bound by RNA polymerase. This effect was due largely to CRP-cAMP repression of RNA polymerase binding to an overlapping site (
lac P2) within the promoter region. In addition, a direct enhancement of RNA polymerase binding at the principal
lac promoter (
lac P1) was found. The combination of indirect and direct activation by CRP-cAMP was suggested to be responsible for the large activation observed
in vivo. Promoter strength parameters were also determined for the L8, UV5 and P
8 promoters. The effect of CRP-cAMP on these mutant promoters was shown to be consistent with the activation mechanism deduced for the
lac wild-type promoter. DNA supercoiling enhanced the promoter strength of the
lac wild-type and UV5 promoters. The combination of supercoiling and CRP-cAMP was necessary for optimal promoter strength for the
lac wild-type promoter.</description><subject>Applied sciences</subject><subject>Cyclic AMP</subject><subject>DNA, Superhelical</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Escherichia coli - genetics</subject><subject>Exact sciences and technology</subject><subject>Kinetics</subject><subject>Lac Operon</subject><subject>Models, Genetic</subject><subject>Other techniques and industries</subject><subject>Receptors, Cyclic AMP</subject><subject>Templates, Genetic</subject><subject>Transcription, Genetic</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMoOl7eQKELEV1UkzRNTzfCMHgDxUF0HdL0BCO9jElG8O1tnWHAjauQ_N9_TvgIOWb0klEmryjlPOWQyXMQFyXlkqdyi0wYhTIFmcE2mWyQPbIfwgelNM8E7JJdSUuQJZsQfELzrjsX2qS3yexlnprp0zzRJrovHV3fjc-NNkm_QD_cotddMN4tfjPXuehW2N9GfMdkzpKF79s-oj8kO1Y3AY_W5wF5u715nd2nj893D7PpY2oykDG1WBbUUmEs01oDcCEwpzVWWW5tVRrOoNCFzWsmMs55ZSlSJsZCBbWWmB2Qs9XcYfHnEkNUrQsGm0Z32C-DKnIAwUEMoFiBxvcheLRq4V2r_bdiVI121ahOjeoUCPVrV8mhdrKev6xarDeltc4hP13nOhjd2EGWcWGDAWSiKPIBu15hOLj4cuhVMA47g7XzaKKqe_f_P34AOPiWrw</recordid><startdate>19841225</startdate><enddate>19841225</enddate><creator>Malan, T.Philip</creator><creator>Kolb, Annie</creator><creator>Buc, Henri</creator><creator>McClure, William R.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19841225</creationdate><title>Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter</title><author>Malan, T.Philip ; Kolb, Annie ; Buc, Henri ; McClure, William R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-fe970f04cf1aaa88244e50deb35ffb9c2187a7f5d143222bf0e014f04cb8da6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Applied sciences</topic><topic>Cyclic AMP</topic><topic>DNA, Superhelical</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Escherichia coli - genetics</topic><topic>Exact sciences and technology</topic><topic>Kinetics</topic><topic>Lac Operon</topic><topic>Models, Genetic</topic><topic>Other techniques and industries</topic><topic>Receptors, Cyclic AMP</topic><topic>Templates, Genetic</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malan, T.Philip</creatorcontrib><creatorcontrib>Kolb, Annie</creatorcontrib><creatorcontrib>Buc, Henri</creatorcontrib><creatorcontrib>McClure, William R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malan, T.Philip</au><au>Kolb, Annie</au><au>Buc, Henri</au><au>McClure, William R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1984-12-25</date><risdate>1984</risdate><volume>180</volume><issue>4</issue><spage>881</spage><epage>909</epage><pages>881-909</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>CRP-cAMP was shown to activate transcription initiation at the
Escherichia coli lac promoter
in vitro as a result of two separate effects. An indirect component of the activation resulted from an enhancement of the fraction of promoters productively bound by RNA polymerase. This effect was due largely to CRP-cAMP repression of RNA polymerase binding to an overlapping site (
lac P2) within the promoter region. In addition, a direct enhancement of RNA polymerase binding at the principal
lac promoter (
lac P1) was found. The combination of indirect and direct activation by CRP-cAMP was suggested to be responsible for the large activation observed
in vivo. Promoter strength parameters were also determined for the L8, UV5 and P
8 promoters. The effect of CRP-cAMP on these mutant promoters was shown to be consistent with the activation mechanism deduced for the
lac wild-type promoter. DNA supercoiling enhanced the promoter strength of the
lac wild-type and UV5 promoters. The combination of supercoiling and CRP-cAMP was necessary for optimal promoter strength for the
lac wild-type promoter.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>6098691</pmid><doi>10.1016/0022-2836(84)90262-6</doi><tpages>29</tpages></addata></record> |
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| ispartof | Journal of molecular biology, 1984-12, Vol.180 (4), p.881-909 |
| issn | 0022-2836 1089-8638 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Applied sciences Cyclic AMP DNA, Superhelical DNA-Directed RNA Polymerases - metabolism Escherichia coli - genetics Exact sciences and technology Kinetics Lac Operon Models, Genetic Other techniques and industries Receptors, Cyclic AMP Templates, Genetic Transcription, Genetic |
| title | Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter |
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