Interaction of pyridoxal phosphate with thymidylate synthase: Spectral and equilibrium dialysis studies

1. 1. Changes in the spectrum of pyridoxal phosphate (PLP) were produced by adding an equimolar amount of native thymidylate synthase, but not by adding denatured enzyme or enzyme modified by sulfhydryl-blocking reagents. 2. 2. The dissociation constant of the thymidylate synthase-PLP complex determ...

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Veröffentlicht in:	International journal of biochemistry 1993-05, Vol.25 (5), p.671-680
Hauptverfasser:	Appley, M.I., Daron, H.H., Aull, J.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry binding Binding Sites Biological and medical sciences denaturation Deoxyuracil Nucleotides - pharmacology Dialysis dissociation Enzymes and enzyme inhibitors equilibrium Fluorodeoxyuridylate - pharmacology Fundamental and applied biological sciences. Psychology interactions Lactobacillus casei pyridoxal phosphate Pyridoxal Phosphate - chemistry Pyridoxal Phosphate - metabolism spectral analysis Spectrophotometry, Ultraviolet thymidine Thymidine Monophosphate - pharmacology Thymidylate Synthase - chemistry Thymidylate Synthase - metabolism transferases
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