Surface-bound TGF-beta1 on effusion-derived exosomes participates in maintenance of number and suppressive function of regulatory T-cells in malignant effusions
This study analysed the contribution of malignant-effusion derived exosomes (Eff-Ex) to the number and function of regulatory T-cells (Treg) in malignant effusions. Eff-Ex were collected from the malignant effusions of 24 cancer patients. Peripheral blood mononuclear cells (PBMCs) were co-cultured w...
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| Veröffentlicht in: | Anticancer research 2010-09, Vol.30 (9), p.3747-3757 |
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| creator | Wada, Junji Onishi, Hideya Suzuki, Hiroyuki Yamasaki, Akio Nagai, Shuntaro Morisaki, Takashi Katano, Mitsuo |
| description | This study analysed the contribution of malignant-effusion derived exosomes (Eff-Ex) to the number and function of regulatory T-cells (Treg) in malignant effusions.
Eff-Ex were collected from the malignant effusions of 24 cancer patients. Peripheral blood mononuclear cells (PBMCs) were co-cultured with different concentrations of Eff-Ex. FOXP3(+) CD4(+) T-cells were defined as Treg. Expression of molecules on Eff-Ex was determined by flow cytometric analysis.
The number of Treg decreased daily in parallel with the FOXP3 expression level. Purified Eff-Ex prevented the decreases in both Treg number and FOXP3 expression levels in a dose-dependent manner. Pre-treatment of Eff-Ex with a neutralizing mAb against TGF-β1 significantly reduced these effects and the suppressive function of Treg.
Elimination of Eff-Ex or control of Eff-Ex expressing TGF-β1 may be new therapeutic strategies in immunotherapy for advanced cancer patients with malignant effusions. |
| format | Article |
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Eff-Ex were collected from the malignant effusions of 24 cancer patients. Peripheral blood mononuclear cells (PBMCs) were co-cultured with different concentrations of Eff-Ex. FOXP3(+) CD4(+) T-cells were defined as Treg. Expression of molecules on Eff-Ex was determined by flow cytometric analysis.
The number of Treg decreased daily in parallel with the FOXP3 expression level. Purified Eff-Ex prevented the decreases in both Treg number and FOXP3 expression levels in a dose-dependent manner. Pre-treatment of Eff-Ex with a neutralizing mAb against TGF-β1 significantly reduced these effects and the suppressive function of Treg.
Elimination of Eff-Ex or control of Eff-Ex expressing TGF-β1 may be new therapeutic strategies in immunotherapy for advanced cancer patients with malignant effusions.</description><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 20944164</identifier><language>eng</language><publisher>Greece</publisher><subject>Cell Separation ; Coculture Techniques ; Enzyme-Linked Immunosorbent Assay ; Exosomes - immunology ; Exosomes - metabolism ; Flow Cytometry ; Humans ; Immunoblotting ; Leukocytes, Mononuclear - immunology ; Neoplasms - immunology ; Neoplasms - metabolism ; Pleural Effusion, Malignant - immunology ; Pleural Effusion, Malignant - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; Transforming Growth Factor beta1 - immunology ; Transforming Growth Factor beta1 - metabolism</subject><ispartof>Anticancer research, 2010-09, Vol.30 (9), p.3747-3757</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20944164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wada, Junji</creatorcontrib><creatorcontrib>Onishi, Hideya</creatorcontrib><creatorcontrib>Suzuki, Hiroyuki</creatorcontrib><creatorcontrib>Yamasaki, Akio</creatorcontrib><creatorcontrib>Nagai, Shuntaro</creatorcontrib><creatorcontrib>Morisaki, Takashi</creatorcontrib><creatorcontrib>Katano, Mitsuo</creatorcontrib><title>Surface-bound TGF-beta1 on effusion-derived exosomes participates in maintenance of number and suppressive function of regulatory T-cells in malignant effusions</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>This study analysed the contribution of malignant-effusion derived exosomes (Eff-Ex) to the number and function of regulatory T-cells (Treg) in malignant effusions.
Eff-Ex were collected from the malignant effusions of 24 cancer patients. Peripheral blood mononuclear cells (PBMCs) were co-cultured with different concentrations of Eff-Ex. FOXP3(+) CD4(+) T-cells were defined as Treg. Expression of molecules on Eff-Ex was determined by flow cytometric analysis.
The number of Treg decreased daily in parallel with the FOXP3 expression level. Purified Eff-Ex prevented the decreases in both Treg number and FOXP3 expression levels in a dose-dependent manner. Pre-treatment of Eff-Ex with a neutralizing mAb against TGF-β1 significantly reduced these effects and the suppressive function of Treg.
Elimination of Eff-Ex or control of Eff-Ex expressing TGF-β1 may be new therapeutic strategies in immunotherapy for advanced cancer patients with malignant effusions.</description><subject>Cell Separation</subject><subject>Coculture Techniques</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Exosomes - immunology</subject><subject>Exosomes - metabolism</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Neoplasms - immunology</subject><subject>Neoplasms - metabolism</subject><subject>Pleural Effusion, Malignant - immunology</subject><subject>Pleural Effusion, Malignant - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>Transforming Growth Factor beta1 - immunology</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFKxDAQhosg7rr6CpKbp0DapGlzlMVdBcGDvZdJO1kibVKTRty38VHt4uppGPjm-3_mIlvnlcppVXK2yq5jfGdMSlXzq2xVMCVELsU6-35LwUCHVPvketLsd1TjDDnxjqAxKVrvaI_BfmJP8MtHP2IkE4TZdnaCeVmsIyNYN6MD1yHxhrg0agwEFmFM0xQwxuWemOS6efGdkICHNMDsw5E0tMNhOHsGe1g08392vMkuDQwRb89zkzW7x2b7RF9e98_bhxc6laWgkNclq_NO6Z4rwwupjSnKnEumhdQgVM1Y0QMiVtIY0MpAyYSRjGkFGg3fZPe_2in4j4RxbkcbT73AoU-xrcq65qJibCHvzmTSI_btFOwI4dj-_ZT_AHaTdoI</recordid><startdate>201009</startdate><enddate>201009</enddate><creator>Wada, Junji</creator><creator>Onishi, Hideya</creator><creator>Suzuki, Hiroyuki</creator><creator>Yamasaki, Akio</creator><creator>Nagai, Shuntaro</creator><creator>Morisaki, Takashi</creator><creator>Katano, Mitsuo</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201009</creationdate><title>Surface-bound TGF-beta1 on effusion-derived exosomes participates in maintenance of number and suppressive function of regulatory T-cells in malignant effusions</title><author>Wada, Junji ; Onishi, Hideya ; Suzuki, Hiroyuki ; Yamasaki, Akio ; Nagai, Shuntaro ; Morisaki, Takashi ; Katano, Mitsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p554-a185081c9bd39f326bff251360b46ba498002daeee76ffab9fa504f600b9abef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Cell Separation</topic><topic>Coculture Techniques</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Exosomes - immunology</topic><topic>Exosomes - metabolism</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Neoplasms - immunology</topic><topic>Neoplasms - metabolism</topic><topic>Pleural Effusion, Malignant - immunology</topic><topic>Pleural Effusion, Malignant - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>Transforming Growth Factor beta1 - immunology</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wada, Junji</creatorcontrib><creatorcontrib>Onishi, Hideya</creatorcontrib><creatorcontrib>Suzuki, Hiroyuki</creatorcontrib><creatorcontrib>Yamasaki, Akio</creatorcontrib><creatorcontrib>Nagai, Shuntaro</creatorcontrib><creatorcontrib>Morisaki, Takashi</creatorcontrib><creatorcontrib>Katano, Mitsuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wada, Junji</au><au>Onishi, Hideya</au><au>Suzuki, Hiroyuki</au><au>Yamasaki, Akio</au><au>Nagai, Shuntaro</au><au>Morisaki, Takashi</au><au>Katano, Mitsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface-bound TGF-beta1 on effusion-derived exosomes participates in maintenance of number and suppressive function of regulatory T-cells in malignant effusions</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2010-09</date><risdate>2010</risdate><volume>30</volume><issue>9</issue><spage>3747</spage><epage>3757</epage><pages>3747-3757</pages><eissn>1791-7530</eissn><abstract>This study analysed the contribution of malignant-effusion derived exosomes (Eff-Ex) to the number and function of regulatory T-cells (Treg) in malignant effusions.
Eff-Ex were collected from the malignant effusions of 24 cancer patients. Peripheral blood mononuclear cells (PBMCs) were co-cultured with different concentrations of Eff-Ex. FOXP3(+) CD4(+) T-cells were defined as Treg. Expression of molecules on Eff-Ex was determined by flow cytometric analysis.
The number of Treg decreased daily in parallel with the FOXP3 expression level. Purified Eff-Ex prevented the decreases in both Treg number and FOXP3 expression levels in a dose-dependent manner. Pre-treatment of Eff-Ex with a neutralizing mAb against TGF-β1 significantly reduced these effects and the suppressive function of Treg.
Elimination of Eff-Ex or control of Eff-Ex expressing TGF-β1 may be new therapeutic strategies in immunotherapy for advanced cancer patients with malignant effusions.</abstract><cop>Greece</cop><pmid>20944164</pmid><tpages>11</tpages></addata></record> |
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| ispartof | Anticancer research, 2010-09, Vol.30 (9), p.3747-3757 |
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| language | eng |
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| source | MEDLINE; EZB Electronic Journals Library |
| subjects | Cell Separation Coculture Techniques Enzyme-Linked Immunosorbent Assay Exosomes - immunology Exosomes - metabolism Flow Cytometry Humans Immunoblotting Leukocytes, Mononuclear - immunology Neoplasms - immunology Neoplasms - metabolism Pleural Effusion, Malignant - immunology Pleural Effusion, Malignant - metabolism Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism Transforming Growth Factor beta1 - immunology Transforming Growth Factor beta1 - metabolism |
| title | Surface-bound TGF-beta1 on effusion-derived exosomes participates in maintenance of number and suppressive function of regulatory T-cells in malignant effusions |
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