In vivo measurement of the potential doubling time by flowcytometry in oropharyngeal cancer treated by conventional radiotherapy
Purpose : Experimental and clinical studies suggest that the pre-treatment potential doubling time could be predictive of tumor control in patients treated by conventional radiotherapy and could help to identify the rapidly growing tumors for which accelerated radiotherapy is required. Methods and M...
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| Veröffentlicht in: | International journal of radiation oncology, biology, physics biology, physics, 1993-08, Vol.26 (5), p.793-799 |
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| container_title | International journal of radiation oncology, biology, physics |
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| creator | Bourhis, J. Wilson, G. Wibault, P. Bosq, J. Chavaudra, N. Janot, F. Luboinski, B. Eschwege, F. Malaise, E.P. |
| description | Purpose
: Experimental and clinical studies suggest that the pre-treatment potential doubling time could be predictive of tumor control in patients treated by conventional radiotherapy and could help to identify the rapidly growing tumors for which accelerated radiotherapy is required.
Methods and Materials
: To test this hypothesis, we studied prospectively 48 patients with a squamous cell carcinoma of the oropharynx and treated by conventional radiotherapy (70 Gy/7 weeks). The duration of S phase, the labeling index and the potential doubling time were obtained by flowcytometry measurements of a tumor biopsy obtained after injection of 200 mg bromodeoxyuridine to the patient.
Results
: Three parameters were significantly associated with an increased risk of relapse namely the tumors size (T4;
p < 0.01), the nodal status (≥ N2;
p < 0.05) and the site of the primary within the oropharynx (
p = 0.08). The S phase, labeling index, DNA index and potential doubling time were not significantly associated with an increased risk of relapse. However when considering only the T2 subgroup of patients, high labeling indexes and short potential doubling time were associated with an increased risk of relapse: the mean pre-treatment potential doubling time of the tumors which relapsed was 3.21 versus 5.5 days when there was no evidence of local relapse (
p < 0.05). The mean labeling index for the group of tumors associated with a tumor recurrence was 11.7% compared to 7.3% when there was no evidence of relapse (
p = 0.02).
Conclusion
: Factors other than proliferation play a role in determining the outcome of oropharyngeal cancers treated by conventional radiotherapy. However there was a significant correlation between short potential doubling time, high labeling index and tumor recurrence in the T2 subgroup of patients. The finding of significance for potential doubling time and labeling index in the T2 subset of tumors may be a reflexion of the more homogeneneous nature of these tumors with regard to prognostic variables. |
| doi_str_mv | 10.1016/0360-3016(93)90494-G |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75882530</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>036030169390494G</els_id><sourcerecordid>75882530</sourcerecordid><originalsourceid>FETCH-LOGICAL-c301t-995f4342a5288e84850257fa66c2c47b4612868c526729cb6e99fde4a63dd0733</originalsourceid><addsrcrecordid>eNp9kE-P1CAYh4nRrLOr30ATDsashyoFSuGyidnouMkmXjTxRii83cW0pQId05sfXepM5ugJyO95__Ag9Kom72tSiw-ECVKxcrtW7J0iXPFq_wTtatmqijXNj6dod0aeo8uUfhJC6rrlF-hCMs4llzv0527CB38IeASTlggjTBmHHudHwHPI5eXNgF1YusFPDzj7EXC34n4Iv-2awwg5rthPOMQwP5q4Tg9QeGsmCxHnCCaD2wpsmA5bszCVOBrnQ5kQzby-QM96MyR4eTqv0PfPn77dfqnuv-7vbj_eV7bsnyulmp4zTk1DpYSyekNo0_ZGCEstbzsuaiqFtA0VLVW2E6BU74AbwZwjLWNX6O2x7xzDrwVS1qNPFobBTBCWpNtGStowUkB-BG0MKUXo9Rz9WL6ma6I38XqzqjerWjH9T7zel7LXp_5LN4I7F51Ml_zNKTfJmqGPRZFPZ4xLSSihBbs5YlBcHDxEnayHYtP5CDZrF_z_9_gLD0-hSw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75882530</pqid></control><display><type>article</type><title>In vivo measurement of the potential doubling time by flowcytometry in oropharyngeal cancer treated by conventional radiotherapy</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Bourhis, J. ; Wilson, G. ; Wibault, P. ; Bosq, J. ; Chavaudra, N. ; Janot, F. ; Luboinski, B. ; Eschwege, F. ; Malaise, E.P.</creator><creatorcontrib>Bourhis, J. ; Wilson, G. ; Wibault, P. ; Bosq, J. ; Chavaudra, N. ; Janot, F. ; Luboinski, B. ; Eschwege, F. ; Malaise, E.P.</creatorcontrib><description>Purpose
: Experimental and clinical studies suggest that the pre-treatment potential doubling time could be predictive of tumor control in patients treated by conventional radiotherapy and could help to identify the rapidly growing tumors for which accelerated radiotherapy is required.
Methods and Materials
: To test this hypothesis, we studied prospectively 48 patients with a squamous cell carcinoma of the oropharynx and treated by conventional radiotherapy (70 Gy/7 weeks). The duration of S phase, the labeling index and the potential doubling time were obtained by flowcytometry measurements of a tumor biopsy obtained after injection of 200 mg bromodeoxyuridine to the patient.
Results
: Three parameters were significantly associated with an increased risk of relapse namely the tumors size (T4;
p < 0.01), the nodal status (≥ N2;
p < 0.05) and the site of the primary within the oropharynx (
p = 0.08). The S phase, labeling index, DNA index and potential doubling time were not significantly associated with an increased risk of relapse. However when considering only the T2 subgroup of patients, high labeling indexes and short potential doubling time were associated with an increased risk of relapse: the mean pre-treatment potential doubling time of the tumors which relapsed was 3.21 versus 5.5 days when there was no evidence of local relapse (
p < 0.05). The mean labeling index for the group of tumors associated with a tumor recurrence was 11.7% compared to 7.3% when there was no evidence of relapse (
p = 0.02).
Conclusion
: Factors other than proliferation play a role in determining the outcome of oropharyngeal cancers treated by conventional radiotherapy. However there was a significant correlation between short potential doubling time, high labeling index and tumor recurrence in the T2 subgroup of patients. The finding of significance for potential doubling time and labeling index in the T2 subset of tumors may be a reflexion of the more homogeneneous nature of these tumors with regard to prognostic variables.</description><identifier>ISSN: 0360-3016</identifier><identifier>EISSN: 1879-355X</identifier><identifier>DOI: 10.1016/0360-3016(93)90494-G</identifier><identifier>PMID: 8344848</identifier><identifier>CODEN: IOBPD3</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Aged ; Biological and medical sciences ; Bromodeoxyuridine ; Carcinoma, Squamous Cell - epidemiology ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - radiotherapy ; Conventional radiotherapy ; Female ; Flow Cytometry ; Humans ; Labeling index ; Male ; Medical sciences ; Middle Aged ; Oropharyngeal Neoplasms - epidemiology ; Oropharyngeal Neoplasms - pathology ; Oropharyngeal Neoplasms - radiotherapy ; Oropharynx ; Otorhinolaryngology (head neck, general aspects and miscellaneous) ; Otorhinolaryngology. Stomatology ; Potential doubling time ; Prospective Studies ; Survival Rate ; Tumors</subject><ispartof>International journal of radiation oncology, biology, physics, 1993-08, Vol.26 (5), p.793-799</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-995f4342a5288e84850257fa66c2c47b4612868c526729cb6e99fde4a63dd0733</citedby><cites>FETCH-LOGICAL-c301t-995f4342a5288e84850257fa66c2c47b4612868c526729cb6e99fde4a63dd0733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0360-3016(93)90494-G$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4880202$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8344848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bourhis, J.</creatorcontrib><creatorcontrib>Wilson, G.</creatorcontrib><creatorcontrib>Wibault, P.</creatorcontrib><creatorcontrib>Bosq, J.</creatorcontrib><creatorcontrib>Chavaudra, N.</creatorcontrib><creatorcontrib>Janot, F.</creatorcontrib><creatorcontrib>Luboinski, B.</creatorcontrib><creatorcontrib>Eschwege, F.</creatorcontrib><creatorcontrib>Malaise, E.P.</creatorcontrib><title>In vivo measurement of the potential doubling time by flowcytometry in oropharyngeal cancer treated by conventional radiotherapy</title><title>International journal of radiation oncology, biology, physics</title><addtitle>Int J Radiat Oncol Biol Phys</addtitle><description>Purpose
: Experimental and clinical studies suggest that the pre-treatment potential doubling time could be predictive of tumor control in patients treated by conventional radiotherapy and could help to identify the rapidly growing tumors for which accelerated radiotherapy is required.
Methods and Materials
: To test this hypothesis, we studied prospectively 48 patients with a squamous cell carcinoma of the oropharynx and treated by conventional radiotherapy (70 Gy/7 weeks). The duration of S phase, the labeling index and the potential doubling time were obtained by flowcytometry measurements of a tumor biopsy obtained after injection of 200 mg bromodeoxyuridine to the patient.
Results
: Three parameters were significantly associated with an increased risk of relapse namely the tumors size (T4;
p < 0.01), the nodal status (≥ N2;
p < 0.05) and the site of the primary within the oropharynx (
p = 0.08). The S phase, labeling index, DNA index and potential doubling time were not significantly associated with an increased risk of relapse. However when considering only the T2 subgroup of patients, high labeling indexes and short potential doubling time were associated with an increased risk of relapse: the mean pre-treatment potential doubling time of the tumors which relapsed was 3.21 versus 5.5 days when there was no evidence of local relapse (
p < 0.05). The mean labeling index for the group of tumors associated with a tumor recurrence was 11.7% compared to 7.3% when there was no evidence of relapse (
p = 0.02).
Conclusion
: Factors other than proliferation play a role in determining the outcome of oropharyngeal cancers treated by conventional radiotherapy. However there was a significant correlation between short potential doubling time, high labeling index and tumor recurrence in the T2 subgroup of patients. The finding of significance for potential doubling time and labeling index in the T2 subset of tumors may be a reflexion of the more homogeneneous nature of these tumors with regard to prognostic variables.</description><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Bromodeoxyuridine</subject><subject>Carcinoma, Squamous Cell - epidemiology</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - radiotherapy</subject><subject>Conventional radiotherapy</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Labeling index</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Oropharyngeal Neoplasms - epidemiology</subject><subject>Oropharyngeal Neoplasms - pathology</subject><subject>Oropharyngeal Neoplasms - radiotherapy</subject><subject>Oropharynx</subject><subject>Otorhinolaryngology (head neck, general aspects and miscellaneous)</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Potential doubling time</subject><subject>Prospective Studies</subject><subject>Survival Rate</subject><subject>Tumors</subject><issn>0360-3016</issn><issn>1879-355X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE-P1CAYh4nRrLOr30ATDsashyoFSuGyidnouMkmXjTxRii83cW0pQId05sfXepM5ugJyO95__Ag9Kom72tSiw-ECVKxcrtW7J0iXPFq_wTtatmqijXNj6dod0aeo8uUfhJC6rrlF-hCMs4llzv0527CB38IeASTlggjTBmHHudHwHPI5eXNgF1YusFPDzj7EXC34n4Iv-2awwg5rthPOMQwP5q4Tg9QeGsmCxHnCCaD2wpsmA5bszCVOBrnQ5kQzby-QM96MyR4eTqv0PfPn77dfqnuv-7vbj_eV7bsnyulmp4zTk1DpYSyekNo0_ZGCEstbzsuaiqFtA0VLVW2E6BU74AbwZwjLWNX6O2x7xzDrwVS1qNPFobBTBCWpNtGStowUkB-BG0MKUXo9Rz9WL6ma6I38XqzqjerWjH9T7zel7LXp_5LN4I7F51Ml_zNKTfJmqGPRZFPZ4xLSSihBbs5YlBcHDxEnayHYtP5CDZrF_z_9_gLD0-hSw</recordid><startdate>19930801</startdate><enddate>19930801</enddate><creator>Bourhis, J.</creator><creator>Wilson, G.</creator><creator>Wibault, P.</creator><creator>Bosq, J.</creator><creator>Chavaudra, N.</creator><creator>Janot, F.</creator><creator>Luboinski, B.</creator><creator>Eschwege, F.</creator><creator>Malaise, E.P.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930801</creationdate><title>In vivo measurement of the potential doubling time by flowcytometry in oropharyngeal cancer treated by conventional radiotherapy</title><author>Bourhis, J. ; Wilson, G. ; Wibault, P. ; Bosq, J. ; Chavaudra, N. ; Janot, F. ; Luboinski, B. ; Eschwege, F. ; Malaise, E.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-995f4342a5288e84850257fa66c2c47b4612868c526729cb6e99fde4a63dd0733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Bromodeoxyuridine</topic><topic>Carcinoma, Squamous Cell - epidemiology</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - radiotherapy</topic><topic>Conventional radiotherapy</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Labeling index</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Oropharyngeal Neoplasms - epidemiology</topic><topic>Oropharyngeal Neoplasms - pathology</topic><topic>Oropharyngeal Neoplasms - radiotherapy</topic><topic>Oropharynx</topic><topic>Otorhinolaryngology (head neck, general aspects and miscellaneous)</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Potential doubling time</topic><topic>Prospective Studies</topic><topic>Survival Rate</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bourhis, J.</creatorcontrib><creatorcontrib>Wilson, G.</creatorcontrib><creatorcontrib>Wibault, P.</creatorcontrib><creatorcontrib>Bosq, J.</creatorcontrib><creatorcontrib>Chavaudra, N.</creatorcontrib><creatorcontrib>Janot, F.</creatorcontrib><creatorcontrib>Luboinski, B.</creatorcontrib><creatorcontrib>Eschwege, F.</creatorcontrib><creatorcontrib>Malaise, E.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of radiation oncology, biology, physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bourhis, J.</au><au>Wilson, G.</au><au>Wibault, P.</au><au>Bosq, J.</au><au>Chavaudra, N.</au><au>Janot, F.</au><au>Luboinski, B.</au><au>Eschwege, F.</au><au>Malaise, E.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo measurement of the potential doubling time by flowcytometry in oropharyngeal cancer treated by conventional radiotherapy</atitle><jtitle>International journal of radiation oncology, biology, physics</jtitle><addtitle>Int J Radiat Oncol Biol Phys</addtitle><date>1993-08-01</date><risdate>1993</risdate><volume>26</volume><issue>5</issue><spage>793</spage><epage>799</epage><pages>793-799</pages><issn>0360-3016</issn><eissn>1879-355X</eissn><coden>IOBPD3</coden><abstract>Purpose
: Experimental and clinical studies suggest that the pre-treatment potential doubling time could be predictive of tumor control in patients treated by conventional radiotherapy and could help to identify the rapidly growing tumors for which accelerated radiotherapy is required.
Methods and Materials
: To test this hypothesis, we studied prospectively 48 patients with a squamous cell carcinoma of the oropharynx and treated by conventional radiotherapy (70 Gy/7 weeks). The duration of S phase, the labeling index and the potential doubling time were obtained by flowcytometry measurements of a tumor biopsy obtained after injection of 200 mg bromodeoxyuridine to the patient.
Results
: Three parameters were significantly associated with an increased risk of relapse namely the tumors size (T4;
p < 0.01), the nodal status (≥ N2;
p < 0.05) and the site of the primary within the oropharynx (
p = 0.08). The S phase, labeling index, DNA index and potential doubling time were not significantly associated with an increased risk of relapse. However when considering only the T2 subgroup of patients, high labeling indexes and short potential doubling time were associated with an increased risk of relapse: the mean pre-treatment potential doubling time of the tumors which relapsed was 3.21 versus 5.5 days when there was no evidence of local relapse (
p < 0.05). The mean labeling index for the group of tumors associated with a tumor recurrence was 11.7% compared to 7.3% when there was no evidence of relapse (
p = 0.02).
Conclusion
: Factors other than proliferation play a role in determining the outcome of oropharyngeal cancers treated by conventional radiotherapy. However there was a significant correlation between short potential doubling time, high labeling index and tumor recurrence in the T2 subgroup of patients. The finding of significance for potential doubling time and labeling index in the T2 subset of tumors may be a reflexion of the more homogeneneous nature of these tumors with regard to prognostic variables.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>8344848</pmid><doi>10.1016/0360-3016(93)90494-G</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0360-3016 |
| ispartof | International journal of radiation oncology, biology, physics, 1993-08, Vol.26 (5), p.793-799 |
| issn | 0360-3016 1879-355X |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Aged Biological and medical sciences Bromodeoxyuridine Carcinoma, Squamous Cell - epidemiology Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - radiotherapy Conventional radiotherapy Female Flow Cytometry Humans Labeling index Male Medical sciences Middle Aged Oropharyngeal Neoplasms - epidemiology Oropharyngeal Neoplasms - pathology Oropharyngeal Neoplasms - radiotherapy Oropharynx Otorhinolaryngology (head neck, general aspects and miscellaneous) Otorhinolaryngology. Stomatology Potential doubling time Prospective Studies Survival Rate Tumors |
| title | In vivo measurement of the potential doubling time by flowcytometry in oropharyngeal cancer treated by conventional radiotherapy |
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