Tryptophan mutants of human C5a anaphylatoxin: A fluorescence anisotropy decay and energy transfer study

Three mutants of the anaphylatoxin C5a were prepared with positions 2, 64 and 70, respectively, substituted by tryptophan. The last mutant was additionally labelled at Cys 27 for fluorescence energy transfer (FET) measurements. The structural integrity and biological activity of the molecules were n...

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Veröffentlicht in:	Biophysical chemistry 1993-05, Vol.46 (3), p.237-248
Hauptverfasser:	Federwisch, M., Wollmer, A., Emde, M., Stühmer, T., Melcher, T., Klos, A., Köhl, J., Bautsch, W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Anaphylatoxins - chemistry Anaphylatoxins - genetics Biological and medical sciences C5a mutants (biosynthetic analogues) Circular Dichroism Comparison with NMR structure Complement Complement C5a - chemistry Complement C5a - genetics Complement peptide C5a Cysteine Energy Transfer Fluorescence energy transfer Fluorescence Polarization Fluorescent Dyes Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Molecular immunology Molecular Sequence Data Mutation Naphthalenesulfonates Recombinant Proteins - chemistry Spectrophotometry, Ultraviolet Sulfhydryl Reagents Time Factors Time-resolved fluorescence Tryptophan - chemistry Tryptophan - genetics
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