Guanine nucleotide-specific phosphate transfer by guanine nucleotide-binding regulatory protein beta-subunits. Characterization of the phosphorylated amino acid

One major substrate protein was phosphorylated with [gamma-32P]GTP in membranes of human leukemia (HL-60) cells. The phosphoprotein comigrated with beta-subunits of heterotrimeric GTP-binding proteins (G proteins) in different gel systems. Upon solubilization of the phosphorylated membranes, the pho...

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Veröffentlicht in:	The Journal of biological chemistry 1993-08, Vol.268 (24), p.18111-18118
Hauptverfasser:	Wieland, T., Nürnberg, B., Ulibarri, I., Kaldenberg-Stasch, S., Schultz, G., Jakobs, K.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Binding and carrier proteins Biological and medical sciences Biological Transport Cell Membrane Diethyl Pyrocarbonate - pharmacology Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology GTP-Binding Proteins - isolation & purification GTP-Binding Proteins - metabolism Guanine Nucleotides - metabolism Guanine Nucleotides - pharmacology Guanosine 5'-O-(3-Thiotriphosphate) - metabolism Guanosine Triphosphate - metabolism Histidine - analogs & derivatives Histidine - analysis Histidine - metabolism Humans Hydroxylamine Hydroxylamines - pharmacology Kinetics Leukemia, Promyelocytic, Acute Macromolecular Substances Magnesium - pharmacology Manganese - pharmacology Membrane Proteins - isolation & purification Membrane Proteins - metabolism Molecular Weight Phosphorus Radioisotopes Phosphorylation Proteins Tumor Cells, Cultured
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