A transformed Vero cell line stably producing the hepatitis B virus surface antigen
We have constructed plasmids in which transcription of the surface antigen gene of the human hepatitis B virus (HBsAg) is under the control of the SV40 early promoter. These plasmids have been used to analyze the expression of the HBsAg gene, both in mouse cells and in African green monkey kidney (V...
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| Veröffentlicht in: | Gene 1984-11, Vol.31 (1), p.49-57 |
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| container_start_page | 49 |
| container_title | Gene |
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| creator | Carloni, Guido Malpièce, Yves Michel, Marie-Louise Le Patezour, Annie Sobczak, Eliane Tiollais, Pierre Streeck, Rolf E. |
| description | We have constructed plasmids in which transcription of the surface antigen gene of the human hepatitis B virus (HBsAg) is under the control of the SV40 early promoter. These plasmids have been used to analyze the expression of the HBsAg gene, both in mouse cells and in African green monkey kidney (Vero) cells. We have established a Vero cell line continuously expressing HBsAg that is indistinguishable from authentic 22 nm HBsAg particles. Post-transcriptional modification of HBsAg mRNA also appears to occur normally in the monkey cells. These cells might be useful as a source of antigen for the preparation of an antihepatitis vaccine. |
| doi_str_mv | 10.1016/0378-1119(84)90194-X |
| format | Article |
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These plasmids have been used to analyze the expression of the HBsAg gene, both in mouse cells and in African green monkey kidney (Vero) cells. We have established a Vero cell line continuously expressing HBsAg that is indistinguishable from authentic 22 nm HBsAg particles. Post-transcriptional modification of HBsAg mRNA also appears to occur normally in the monkey cells. These cells might be useful as a source of antigen for the preparation of an antihepatitis vaccine.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(84)90194-X</identifier><identifier>PMID: 6098537</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Animals ; anti-hepatitis vaccine ; Biological and medical sciences ; Biotechnology ; Cell Line ; Cercopithecus aethiops ; cloning ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; gene transfer ; Genetic Vectors ; Health. Pharmaceutical industry ; Hepatitis B Surface Antigens - genetics ; Hepatitis B virus - genetics ; Industrial applications and implications. Economical aspects ; Kidney ; L Cells (Cell Line) ; Mice ; Microbiology ; Production of active biomolecules ; Promoter Regions, Genetic ; Recombinant DNA ; RNA Processing, Post-Transcriptional ; RNA, Messenger - metabolism ; RNA, Viral - metabolism ; SV40 early promoter ; Transcription, Genetic ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccins ; Virology</subject><ispartof>Gene, 1984-11, Vol.31 (1), p.49-57</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-9c719b768d6a1e4e0ffa5542c5ca5646a590433addff0acee985e87854b53f0d3</citedby><cites>FETCH-LOGICAL-c386t-9c719b768d6a1e4e0ffa5542c5ca5646a590433addff0acee985e87854b53f0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(84)90194-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9105681$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6098537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carloni, Guido</creatorcontrib><creatorcontrib>Malpièce, Yves</creatorcontrib><creatorcontrib>Michel, Marie-Louise</creatorcontrib><creatorcontrib>Le Patezour, Annie</creatorcontrib><creatorcontrib>Sobczak, Eliane</creatorcontrib><creatorcontrib>Tiollais, Pierre</creatorcontrib><creatorcontrib>Streeck, Rolf E.</creatorcontrib><title>A transformed Vero cell line stably producing the hepatitis B virus surface antigen</title><title>Gene</title><addtitle>Gene</addtitle><description>We have constructed plasmids in which transcription of the surface antigen gene of the human hepatitis B virus (HBsAg) is under the control of the SV40 early promoter. These plasmids have been used to analyze the expression of the HBsAg gene, both in mouse cells and in African green monkey kidney (Vero) cells. We have established a Vero cell line continuously expressing HBsAg that is indistinguishable from authentic 22 nm HBsAg particles. Post-transcriptional modification of HBsAg mRNA also appears to occur normally in the monkey cells. These cells might be useful as a source of antigen for the preparation of an antihepatitis vaccine.</description><subject>Animals</subject><subject>anti-hepatitis vaccine</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>Cercopithecus aethiops</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>gene transfer</subject><subject>Genetic Vectors</subject><subject>Health. Pharmaceutical industry</subject><subject>Hepatitis B Surface Antigens - genetics</subject><subject>Hepatitis B virus - genetics</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Kidney</subject><subject>L Cells (Cell Line)</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Production of active biomolecules</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant DNA</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Viral - metabolism</subject><subject>SV40 early promoter</subject><subject>Transcription, Genetic</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccins</subject><subject>Virology</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKBDEQRYMoOj7-QCELEV20JnaeG2EUXyC48IG7kElXNNLTPSZpwb834wyztDa1qFOXy0Fon5JTSqg4I7VUFaVUHyt2ognVrHpbQyOqpK4IqdU6Gq2QLbSd0icpw_n5JtoURCteyxF6GuMcbZd8H6fQ4FeIPXbQtrgNHeCU7aT9wbPYN4ML3TvOH4A_YGZzyCHhS_wd4pBwGqK3DrDtcniHbhdteNsm2FvuHfRyc_18dVc9PN7eX40fKlcrkSvtJNUTKVQjLAUGxHvLOTt33FkumLBcE1bXtmm8JyUeSmVQUnE24bUnTb2Djha5pd_XACmbaUjz8raDfkhGciUZEbKAbAG62KcUwZtZDFMbfwwlZu7SzEWZuSijmPlzad7K28Eyf5gUOaunpbxyP1zebXK29cWjC2mFaUq4ULRgFwsMiovvANEkF6Bz0IQILpumD__3-AXL7pCa</recordid><startdate>198411</startdate><enddate>198411</enddate><creator>Carloni, Guido</creator><creator>Malpièce, Yves</creator><creator>Michel, Marie-Louise</creator><creator>Le Patezour, Annie</creator><creator>Sobczak, Eliane</creator><creator>Tiollais, Pierre</creator><creator>Streeck, Rolf E.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198411</creationdate><title>A transformed Vero cell line stably producing the hepatitis B virus surface antigen</title><author>Carloni, Guido ; Malpièce, Yves ; Michel, Marie-Louise ; Le Patezour, Annie ; Sobczak, Eliane ; Tiollais, Pierre ; Streeck, Rolf E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-9c719b768d6a1e4e0ffa5542c5ca5646a590433addff0acee985e87854b53f0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>anti-hepatitis vaccine</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>Cercopithecus aethiops</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>gene transfer</topic><topic>Genetic Vectors</topic><topic>Health. Pharmaceutical industry</topic><topic>Hepatitis B Surface Antigens - genetics</topic><topic>Hepatitis B virus - genetics</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Kidney</topic><topic>L Cells (Cell Line)</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Production of active biomolecules</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant DNA</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Viral - metabolism</topic><topic>SV40 early promoter</topic><topic>Transcription, Genetic</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccins</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carloni, Guido</creatorcontrib><creatorcontrib>Malpièce, Yves</creatorcontrib><creatorcontrib>Michel, Marie-Louise</creatorcontrib><creatorcontrib>Le Patezour, Annie</creatorcontrib><creatorcontrib>Sobczak, Eliane</creatorcontrib><creatorcontrib>Tiollais, Pierre</creatorcontrib><creatorcontrib>Streeck, Rolf E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carloni, Guido</au><au>Malpièce, Yves</au><au>Michel, Marie-Louise</au><au>Le Patezour, Annie</au><au>Sobczak, Eliane</au><au>Tiollais, Pierre</au><au>Streeck, Rolf E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A transformed Vero cell line stably producing the hepatitis B virus surface antigen</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1984-11</date><risdate>1984</risdate><volume>31</volume><issue>1</issue><spage>49</spage><epage>57</epage><pages>49-57</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>We have constructed plasmids in which transcription of the surface antigen gene of the human hepatitis B virus (HBsAg) is under the control of the SV40 early promoter. These plasmids have been used to analyze the expression of the HBsAg gene, both in mouse cells and in African green monkey kidney (Vero) cells. We have established a Vero cell line continuously expressing HBsAg that is indistinguishable from authentic 22 nm HBsAg particles. Post-transcriptional modification of HBsAg mRNA also appears to occur normally in the monkey cells. These cells might be useful as a source of antigen for the preparation of an antihepatitis vaccine.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>6098537</pmid><doi>10.1016/0378-1119(84)90194-X</doi><tpages>9</tpages></addata></record> |
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| ispartof | Gene, 1984-11, Vol.31 (1), p.49-57 |
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| subjects | Animals anti-hepatitis vaccine Biological and medical sciences Biotechnology Cell Line Cercopithecus aethiops cloning Cloning, Molecular Fundamental and applied biological sciences. Psychology Gene Expression Regulation gene transfer Genetic Vectors Health. Pharmaceutical industry Hepatitis B Surface Antigens - genetics Hepatitis B virus - genetics Industrial applications and implications. Economical aspects Kidney L Cells (Cell Line) Mice Microbiology Production of active biomolecules Promoter Regions, Genetic Recombinant DNA RNA Processing, Post-Transcriptional RNA, Messenger - metabolism RNA, Viral - metabolism SV40 early promoter Transcription, Genetic Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccins Virology |
| title | A transformed Vero cell line stably producing the hepatitis B virus surface antigen |
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