Targeting TBP to a non-TATA box cis-regulatory element : a TBP-containing complex activates transcription from snRNA promoters through the PSE

In the human small nuclear RNA (snRNA) promoters, the presence of a TATA box recognized by the TATA box-binding protein (TBP) determines the selection of RNA polymerase III over RNA polymerase II. The RNA polymerase II snRNA promoters are, therefore, good candidates for TBP-independent promoters. We...

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Veröffentlicht in:Genes & development 1993-08, Vol.7 (8), p.1535-1548
Hauptverfasser: SADOWSKI, C. L, HENRY, R. W, HERNANDEZ, N
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container_title Genes & development
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creator SADOWSKI, C. L
HENRY, R. W
HERNANDEZ, N
description In the human small nuclear RNA (snRNA) promoters, the presence of a TATA box recognized by the TATA box-binding protein (TBP) determines the selection of RNA polymerase III over RNA polymerase II. The RNA polymerase II snRNA promoters are, therefore, good candidates for TBP-independent promoters. We show here, however, that TBP activates transcription from RNA polymerase II snRNA promoters through a non-TATA box element, the snRNA proximal sequence element (PSE), as part of a new snRNA-activating protein complex (SNAPc). In contrast to the previously identified TBP-containing complexes SL1, TFIID, and TFIIIB, which appear dedicated to transcription by a single RNA polymerase, SNAPc is also essential for RNA polymerase III transcription from the U6 snRNA promoter. The U6 initiation complex appears to contain two forms of TBP, one bound to the TATA box and one bound to the PSE as a part of SNAPc, suggesting that multiple TBP molecules can have different functions within a single promoter.
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source MEDLINE; EZB-FREE-00999 freely available EZB journals
subjects Animals
Base Sequence
Biological and medical sciences
Cross-Linking Reagents
DNA-Binding Proteins - metabolism
DNA-Directed RNA Polymerases - biosynthesis
Fundamental and applied biological sciences. Psychology
Humans
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Promoter Regions, Genetic
Proteins
Ribonucleoprotein, U4-U6 Small Nuclear - genetics
RNA Polymerase II - biosynthesis
RNA Polymerase III - biosynthesis
Structure-Activity Relationship
TATA Box
TATA-Box Binding Protein
Trans-Activators - metabolism
Transcription Factors - metabolism
Transcription. Transcription factor. Splicing. Rna processing
Transcriptional Activation
title Targeting TBP to a non-TATA box cis-regulatory element : a TBP-containing complex activates transcription from snRNA promoters through the PSE
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