Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique
DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) ana...
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| Veröffentlicht in: | Forensic science international 1993-06, Vol.60 (1), p.47-56 |
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| creator | Thymann, Mariann Nellemann, Lars J. Masumba, Grethe Irgens-Møller, Lone Morling, Niels |
| description | DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) analysis of the D1S80 locus demonstrated 21 alleles and a heterozygosity of 77%. Of the 231 possible phenotypes, 57 were observed. All mother/child pairs shared at least one D1S80 allele. The D1S80 typing results in 70 Danes were compared to the results obtained on the same samples in another laboratory and the results were concordant in all cases. |
| doi_str_mv | 10.1016/0379-0738(93)90091-N |
| format | Article |
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Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique</title><title>Forensic science international</title><addtitle>Forensic Sci Int</addtitle><description>DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) analysis of the D1S80 locus demonstrated 21 alleles and a heterozygosity of 77%. Of the 231 possible phenotypes, 57 were observed. All mother/child pairs shared at least one D1S80 allele. The D1S80 typing results in 70 Danes were compared to the results obtained on the same samples in another laboratory and the results were concordant in all cases.</description><subject>Adult</subject><subject>Alleles</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>D1S80</subject><subject>Denmark</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>European Continental Ancestry Group - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Human</subject><subject>Humans</subject><subject>Male</subject><subject>Mothers</subject><subject>Paternity</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Population data, Danish Caucasians</subject><subject>Reproducibility of Results</subject><subject>Variable number of tandem repeats</subject><issn>0379-0738</issn><issn>1872-6283</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhiMEKkvhDUDyAaH2kMWOncS-IK3abqm0lErA2XKcSdfIsbe2g5Qn4_XwssseOVma-Wfm9_8VxVuClwST5iOmrShxS_mFoJcCY0HK-2fFgvC2KpuK0-fF4iR5WbyK8SfGuK6r5qw443kDr8Si-L1yys7RROQHlLaArNdTRNfkG8eom5Ead9YMBno0BPU4gkvIgntMW7Tzdh592G1NHFECvXXmaQJ0sfryUK43D5dLtA6QK07PqDcxBdNNyXiHjEPXykFcojuXgkLK9bmWICCrOh9U8mFGAXbB95M2nbEmzf_cne68Ll4MykZ4c3zPix_rm-9Xn8vN19u7q9Wm1IyTVA5MNDVVpOK8zhExNSgmKCdctB3FdQOsowP0HeEgoFMVg7bnVEHX9qyGuqXnxYfD3mwnn41JjiZqsDb_wE9RtjVvMKEkC9lBqIOPMcAgd8GMKsySYLnnJfcw5B6GFFT-5SXv89i74_6pG6E_DR0B5f77Y19FrWyG4LSJJxnjmDHBs-zTQQY5i18Ggoza5OihNwF0kr03__fxBwuytDE</recordid><startdate>19930601</startdate><enddate>19930601</enddate><creator>Thymann, Mariann</creator><creator>Nellemann, Lars J.</creator><creator>Masumba, Grethe</creator><creator>Irgens-Møller, Lone</creator><creator>Morling, Niels</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930601</creationdate><title>Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique</title><author>Thymann, Mariann ; Nellemann, Lars J. ; Masumba, Grethe ; Irgens-Møller, Lone ; Morling, Niels</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-f49653a128859004afa49381897b3056e4b3fedb18e9eba24e7d83aeb7d45e573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adult</topic><topic>Alleles</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>D1S80</topic><topic>Denmark</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>European Continental Ancestry Group - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Human</topic><topic>Humans</topic><topic>Male</topic><topic>Mothers</topic><topic>Paternity</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Population data, Danish Caucasians</topic><topic>Reproducibility of Results</topic><topic>Variable number of tandem repeats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thymann, Mariann</creatorcontrib><creatorcontrib>Nellemann, Lars J.</creatorcontrib><creatorcontrib>Masumba, Grethe</creatorcontrib><creatorcontrib>Irgens-Møller, Lone</creatorcontrib><creatorcontrib>Morling, Niels</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Forensic science international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thymann, Mariann</au><au>Nellemann, Lars J.</au><au>Masumba, Grethe</au><au>Irgens-Møller, Lone</au><au>Morling, Niels</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique</atitle><jtitle>Forensic science international</jtitle><addtitle>Forensic Sci Int</addtitle><date>1993-06-01</date><risdate>1993</risdate><volume>60</volume><issue>1</issue><spage>47</spage><epage>56</epage><pages>47-56</pages><issn>0379-0738</issn><eissn>1872-6283</eissn><coden>FSINDR</coden><abstract>DNA from the locus D1S80 was amplified by polymerase chain reaction (PCR) and analyzed by electrophoresis in vertical polyacrylamide gels followed by silverstaining. DNA samples from 119 unrelated Danes and 97 mother/child pairs were examined. The amplified fragment length polymorphism (AMP-FLP) analysis of the D1S80 locus demonstrated 21 alleles and a heterozygosity of 77%. Of the 231 possible phenotypes, 57 were observed. All mother/child pairs shared at least one D1S80 allele. The D1S80 typing results in 70 Danes were compared to the results obtained on the same samples in another laboratory and the results were concordant in all cases.</abstract><cop>Kidlington</cop><pub>Elsevier Ireland Ltd</pub><pmid>8101829</pmid><doi>10.1016/0379-0738(93)90091-N</doi><tpages>10</tpages></addata></record> |
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| ispartof | Forensic science international, 1993-06, Vol.60 (1), p.47-56 |
| issn | 0379-0738 1872-6283 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Alleles Biological and medical sciences Child Classical genetics, quantitative genetics, hybrids D1S80 Denmark DNA DNA - analysis European Continental Ancestry Group - genetics Female Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Human Humans Male Mothers Paternity Phenotype Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Population data, Danish Caucasians Reproducibility of Results Variable number of tandem repeats |
| title | Analysis of the locus D1S80 by amplified fragment length polymorphism technique (AMP-FLP). Frequency distribution in Danes. Intra and inter laboratory reproducibility of the technique |
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