Phospholipase C-induced aggregation and fusion of cholesterol-lecithin small unilamellar vesicles

We have investigated the effects of the Ca2+ -requiring enzyme phospholipase C on the stability of sonicated vesicles made with different molar ratios of cholesterol to lecithin. Vesicle aggregation is detected by following turbidity with time. Upon the addition of phospholipase C and after a short...

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Veröffentlicht in:	Biochemistry (Easton) 1993-07, Vol.32 (27), p.6965-6973
Hauptverfasser:	Luk, Andrew S, Kaler, Eric W, Lee, Sum P
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Artificial membranes and reconstituted systems Biological and medical sciences CHOLESTEROL Cholesterol - metabolism COLESTEROL ESTERASAS ESTERASE Fluorescent Dyes Fundamental and applied biological sciences. Psychology GLICERIDOS GLYCERIDE Kinetics LECITHINE LECITINAS Lipid Bilayers MEMBRANA MEMBRANE Membrane Fusion Membrane physicochemistry Molecular biophysics Naphthalenes - chemistry Phosphatidylcholines - metabolism Pyridinium Compounds - chemistry Type C Phospholipases - metabolism
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