A Rabbit Model for Human Cytomegalovirus-Induced Chorioretinal Disease

AD169, a well-characterized laboratory strain of human cytomegalovirus (HCMV), was used to establish an animal model of progressive HCMV chorioretinal disease by injection of 105 pfu into the rabbit vitreous. Chorioretinal, vitreous, and pulmonary disease were monitored by HCMV recovery, clinical ob...

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Veröffentlicht in:The Journal of infectious diseases 1993-08, Vol.168 (2), p.336-344
Hauptverfasser: Dunkel, Edmund C., Freitas, Denise de, Scheer, David I., Siegel, Michael L., Zhu, Qi, Whitley, Richard J ., Schaffer, Priscilla A., Pavan-Langston, Deborah
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container_end_page 344
container_issue 2
container_start_page 336
container_title The Journal of infectious diseases
container_volume 168
creator Dunkel, Edmund C.
Freitas, Denise de
Scheer, David I.
Siegel, Michael L.
Zhu, Qi
Whitley, Richard J .
Schaffer, Priscilla A.
Pavan-Langston, Deborah
description AD169, a well-characterized laboratory strain of human cytomegalovirus (HCMV), was used to establish an animal model of progressive HCMV chorioretinal disease by injection of 105 pfu into the rabbit vitreous. Chorioretinal, vitreous, and pulmonary disease were monitored by HCMV recovery, clinical observation, antigen localization, and histopathology. Vitritis and focal areas ofimmune cellular infiltrates were seen in inner retinal layers on days 2-4 after inoculation. Disease progressed with more severe vitritis and to involve the outer retinal layers in areas of mixed monocytic cellular infiltrates, retinal destruction, choroidal edema, and congestion. HCMV was recovered from chorioretinal cell sonicate cultures in titers ranging from 104 to 105 pfu during peak disease, and HCMV antigens were detected focally by immunofluorescence in retinal layers on days 2 and 4 after inoculation. A rabbit model of HCMV chorioretinitis similar to human CMV disease allows investigation of HCMV pathogenesis and new antiviral therapies and evaluation of immune system modulation of the HCMV ocular infection.
doi_str_mv 10.1093/infdis/168.2.336
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subjects Animal models
Animals
Antigens
Biological and medical sciences
Cells, Cultured
Chorioretinitis - microbiology
Chorioretinitis - pathology
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - complications
Disease models
Disease Models, Animal
Experimental viral diseases and models
Eye diseases
Eye Infections, Viral - microbiology
Eyes
Fluorescent Antibody Technique
Guinea Pigs
Humans
Infections
Infectious diseases
Inoculation
Medical sciences
Pathology
Rabbits
Retinal diseases
Retinitis
Viral diseases
title A Rabbit Model for Human Cytomegalovirus-Induced Chorioretinal Disease
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