A Rabbit Model for Human Cytomegalovirus-Induced Chorioretinal Disease
AD169, a well-characterized laboratory strain of human cytomegalovirus (HCMV), was used to establish an animal model of progressive HCMV chorioretinal disease by injection of 105 pfu into the rabbit vitreous. Chorioretinal, vitreous, and pulmonary disease were monitored by HCMV recovery, clinical ob...
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Veröffentlicht in: | The Journal of infectious diseases 1993-08, Vol.168 (2), p.336-344 |
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creator | Dunkel, Edmund C. Freitas, Denise de Scheer, David I. Siegel, Michael L. Zhu, Qi Whitley, Richard J . Schaffer, Priscilla A. Pavan-Langston, Deborah |
description | AD169, a well-characterized laboratory strain of human cytomegalovirus (HCMV), was used to establish an animal model of progressive HCMV chorioretinal disease by injection of 105 pfu into the rabbit vitreous. Chorioretinal, vitreous, and pulmonary disease were monitored by HCMV recovery, clinical observation, antigen localization, and histopathology. Vitritis and focal areas ofimmune cellular infiltrates were seen in inner retinal layers on days 2-4 after inoculation. Disease progressed with more severe vitritis and to involve the outer retinal layers in areas of mixed monocytic cellular infiltrates, retinal destruction, choroidal edema, and congestion. HCMV was recovered from chorioretinal cell sonicate cultures in titers ranging from 104 to 105 pfu during peak disease, and HCMV antigens were detected focally by immunofluorescence in retinal layers on days 2 and 4 after inoculation. A rabbit model of HCMV chorioretinitis similar to human CMV disease allows investigation of HCMV pathogenesis and new antiviral therapies and evaluation of immune system modulation of the HCMV ocular infection. |
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Chorioretinal, vitreous, and pulmonary disease were monitored by HCMV recovery, clinical observation, antigen localization, and histopathology. Vitritis and focal areas ofimmune cellular infiltrates were seen in inner retinal layers on days 2-4 after inoculation. Disease progressed with more severe vitritis and to involve the outer retinal layers in areas of mixed monocytic cellular infiltrates, retinal destruction, choroidal edema, and congestion. HCMV was recovered from chorioretinal cell sonicate cultures in titers ranging from 104 to 105 pfu during peak disease, and HCMV antigens were detected focally by immunofluorescence in retinal layers on days 2 and 4 after inoculation. A rabbit model of HCMV chorioretinitis similar to human CMV disease allows investigation of HCMV pathogenesis and new antiviral therapies and evaluation of immune system modulation of the HCMV ocular infection.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/168.2.336</identifier><identifier>PMID: 8393056</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animal models ; Animals ; Antigens ; Biological and medical sciences ; Cells, Cultured ; Chorioretinitis - microbiology ; Chorioretinitis - pathology ; Cytomegalovirus - isolation & purification ; Cytomegalovirus Infections - complications ; Disease models ; Disease Models, Animal ; Experimental viral diseases and models ; Eye diseases ; Eye Infections, Viral - microbiology ; Eyes ; Fluorescent Antibody Technique ; Guinea Pigs ; Humans ; Infections ; Infectious diseases ; Inoculation ; Medical sciences ; Pathology ; Rabbits ; Retinal diseases ; Retinitis ; Viral diseases</subject><ispartof>The Journal of infectious diseases, 1993-08, Vol.168 (2), p.336-344</ispartof><rights>Copyright 1993 The University of Chicago</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-3ea6a8653f38ec0614906054aa72bd51f297592cad1f8ebd497889cf0f1b3a133</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30113132$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30113132$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27922,27923,58015,58248</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3794650$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8393056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dunkel, Edmund C.</creatorcontrib><creatorcontrib>Freitas, Denise de</creatorcontrib><creatorcontrib>Scheer, David I.</creatorcontrib><creatorcontrib>Siegel, Michael L.</creatorcontrib><creatorcontrib>Zhu, Qi</creatorcontrib><creatorcontrib>Whitley, Richard J .</creatorcontrib><creatorcontrib>Schaffer, Priscilla A.</creatorcontrib><creatorcontrib>Pavan-Langston, Deborah</creatorcontrib><title>A Rabbit Model for Human Cytomegalovirus-Induced Chorioretinal Disease</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>AD169, a well-characterized laboratory strain of human cytomegalovirus (HCMV), was used to establish an animal model of progressive HCMV chorioretinal disease by injection of 105 pfu into the rabbit vitreous. Chorioretinal, vitreous, and pulmonary disease were monitored by HCMV recovery, clinical observation, antigen localization, and histopathology. Vitritis and focal areas ofimmune cellular infiltrates were seen in inner retinal layers on days 2-4 after inoculation. Disease progressed with more severe vitritis and to involve the outer retinal layers in areas of mixed monocytic cellular infiltrates, retinal destruction, choroidal edema, and congestion. HCMV was recovered from chorioretinal cell sonicate cultures in titers ranging from 104 to 105 pfu during peak disease, and HCMV antigens were detected focally by immunofluorescence in retinal layers on days 2 and 4 after inoculation. A rabbit model of HCMV chorioretinitis similar to human CMV disease allows investigation of HCMV pathogenesis and new antiviral therapies and evaluation of immune system modulation of the HCMV ocular infection.</description><subject>Animal models</subject><subject>Animals</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chorioretinitis - microbiology</subject><subject>Chorioretinitis - pathology</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>Cytomegalovirus Infections - complications</subject><subject>Disease models</subject><subject>Disease Models, Animal</subject><subject>Experimental viral diseases and models</subject><subject>Eye diseases</subject><subject>Eye Infections, Viral - microbiology</subject><subject>Eyes</subject><subject>Fluorescent Antibody Technique</subject><subject>Guinea Pigs</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Inoculation</subject><subject>Medical sciences</subject><subject>Pathology</subject><subject>Rabbits</subject><subject>Retinal diseases</subject><subject>Retinitis</subject><subject>Viral diseases</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtv1EAUhUcIFJaFngbJBaLzZq6v51VGC8lGLELiISGa0bU9AxNsT5ixo-TfY7RLUlKd4jvnFB9jL4FvgBs8DaPvQj4FqTfVBlE-YisQqEopAR-zFedVVYI25il7lvMV57xGqU7YiUaDXMgVOz8rPlHThKn4EDvXFz6mYjcPNBbbuykO7gf18SakOZeXYze3riu2P2MKMbkpjNQXb0N2lN1z9sRTn92LY67Z1_N3X7a7cv_x4nJ7ti_bGuqpREeStBToUbuWS6gNl1zURKpqOgG-MkqYqqUOvHZNVxultWk999AgAeKavTn8Xqf4e3Z5skPIret7Gl2cs1VCCwG1-m8RpFy6i6U144dim2LOyXl7ncJA6c4Ct38d24PjZaFtZRfHy-TV8XtuBtfdD45SF_76yCm31PtEY7sc_KuhMrUU_OHmKk8xPWAOgIDVwssDD3lyt_ec0i8rFSphd9--24vPsN8DB_se_wArK50w</recordid><startdate>19930801</startdate><enddate>19930801</enddate><creator>Dunkel, Edmund C.</creator><creator>Freitas, Denise de</creator><creator>Scheer, David I.</creator><creator>Siegel, Michael L.</creator><creator>Zhu, Qi</creator><creator>Whitley, Richard J .</creator><creator>Schaffer, Priscilla A.</creator><creator>Pavan-Langston, Deborah</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19930801</creationdate><title>A Rabbit Model for Human Cytomegalovirus-Induced Chorioretinal Disease</title><author>Dunkel, Edmund C. ; Freitas, Denise de ; Scheer, David I. ; Siegel, Michael L. ; Zhu, Qi ; Whitley, Richard J . ; Schaffer, Priscilla A. ; Pavan-Langston, Deborah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-3ea6a8653f38ec0614906054aa72bd51f297592cad1f8ebd497889cf0f1b3a133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chorioretinitis - microbiology</topic><topic>Chorioretinitis - pathology</topic><topic>Cytomegalovirus - isolation & purification</topic><topic>Cytomegalovirus Infections - complications</topic><topic>Disease models</topic><topic>Disease Models, Animal</topic><topic>Experimental viral diseases and models</topic><topic>Eye diseases</topic><topic>Eye Infections, Viral - microbiology</topic><topic>Eyes</topic><topic>Fluorescent Antibody Technique</topic><topic>Guinea Pigs</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Inoculation</topic><topic>Medical sciences</topic><topic>Pathology</topic><topic>Rabbits</topic><topic>Retinal diseases</topic><topic>Retinitis</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dunkel, Edmund C.</creatorcontrib><creatorcontrib>Freitas, Denise de</creatorcontrib><creatorcontrib>Scheer, David I.</creatorcontrib><creatorcontrib>Siegel, Michael L.</creatorcontrib><creatorcontrib>Zhu, Qi</creatorcontrib><creatorcontrib>Whitley, Richard J .</creatorcontrib><creatorcontrib>Schaffer, Priscilla A.</creatorcontrib><creatorcontrib>Pavan-Langston, Deborah</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dunkel, Edmund C.</au><au>Freitas, Denise de</au><au>Scheer, David I.</au><au>Siegel, Michael L.</au><au>Zhu, Qi</au><au>Whitley, Richard J .</au><au>Schaffer, Priscilla A.</au><au>Pavan-Langston, Deborah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Rabbit Model for Human Cytomegalovirus-Induced Chorioretinal Disease</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1993-08-01</date><risdate>1993</risdate><volume>168</volume><issue>2</issue><spage>336</spage><epage>344</epage><pages>336-344</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>AD169, a well-characterized laboratory strain of human cytomegalovirus (HCMV), was used to establish an animal model of progressive HCMV chorioretinal disease by injection of 105 pfu into the rabbit vitreous. Chorioretinal, vitreous, and pulmonary disease were monitored by HCMV recovery, clinical observation, antigen localization, and histopathology. Vitritis and focal areas ofimmune cellular infiltrates were seen in inner retinal layers on days 2-4 after inoculation. Disease progressed with more severe vitritis and to involve the outer retinal layers in areas of mixed monocytic cellular infiltrates, retinal destruction, choroidal edema, and congestion. HCMV was recovered from chorioretinal cell sonicate cultures in titers ranging from 104 to 105 pfu during peak disease, and HCMV antigens were detected focally by immunofluorescence in retinal layers on days 2 and 4 after inoculation. A rabbit model of HCMV chorioretinitis similar to human CMV disease allows investigation of HCMV pathogenesis and new antiviral therapies and evaluation of immune system modulation of the HCMV ocular infection.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>8393056</pmid><doi>10.1093/infdis/168.2.336</doi><tpages>9</tpages></addata></record> |
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subjects | Animal models Animals Antigens Biological and medical sciences Cells, Cultured Chorioretinitis - microbiology Chorioretinitis - pathology Cytomegalovirus - isolation & purification Cytomegalovirus Infections - complications Disease models Disease Models, Animal Experimental viral diseases and models Eye diseases Eye Infections, Viral - microbiology Eyes Fluorescent Antibody Technique Guinea Pigs Humans Infections Infectious diseases Inoculation Medical sciences Pathology Rabbits Retinal diseases Retinitis Viral diseases |
title | A Rabbit Model for Human Cytomegalovirus-Induced Chorioretinal Disease |
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