Biochemical Functionality and Recovery of Hepatocytes after Deep Freezing Storage
The present study was undertaken to define the conditions for optimal cryopreservation of hepatocytes. Two different freezing procedures were analyzed: a slow freezing rate (SFR) (-2° C/min down to -30° C and then quick freezing to -196° C) and a fast freezing rate (FFR) (direct freezing of tubes to...
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| Veröffentlicht in: | In Vitro 1984-11, Vol.20 (11), p.826-832 |
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| creator | M. Jose Gomez-L. Lopez, Pilar Jose V. Castell |
| description | The present study was undertaken to define the conditions for optimal cryopreservation of hepatocytes. Two different freezing procedures were analyzed: a slow freezing rate (SFR) (-2° C/min down to -30° C and then quick freezing to -196° C) and a fast freezing rate (FFR) (direct freezing of tubes to -196° C: -39° C/min). Cells were frozen in fetal bovine serum containing 10% Dimethyl sulfoxide (DMSO). After rapid thawing at 37° C, followed by dilution and removal of the cryoprotectant, cells were plated and several parameters were followed as criteria for optimal cryopreservation of cells. The FFR cells showed no apparent ultrastructural damage after 24 h of culture. Plating efficiency and spreading were similar as controls. Gluconeogenesis from pyruvate and fructose, tyrosine amino transferase induction by glucagon and dexamethasone, urea production, and plasma protein synthesis of FFR cells were similar to those found in control cultures. The FFR procedure, in comparison to the SFR method, seemed to render the best preserved hepatocytes. |
| doi_str_mv | 10.1007/bf02619627 |
| format | Article |
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Jose Gomez-L. ; Lopez, Pilar ; Jose V. Castell</creator><creatorcontrib>M. Jose Gomez-L. ; Lopez, Pilar ; Jose V. Castell</creatorcontrib><description>The present study was undertaken to define the conditions for optimal cryopreservation of hepatocytes. Two different freezing procedures were analyzed: a slow freezing rate (SFR) (-2° C/min down to -30° C and then quick freezing to -196° C) and a fast freezing rate (FFR) (direct freezing of tubes to -196° C: -39° C/min). Cells were frozen in fetal bovine serum containing 10% Dimethyl sulfoxide (DMSO). After rapid thawing at 37° C, followed by dilution and removal of the cryoprotectant, cells were plated and several parameters were followed as criteria for optimal cryopreservation of cells. The FFR cells showed no apparent ultrastructural damage after 24 h of culture. Plating efficiency and spreading were similar as controls. Gluconeogenesis from pyruvate and fructose, tyrosine amino transferase induction by glucagon and dexamethasone, urea production, and plasma protein synthesis of FFR cells were similar to those found in control cultures. The FFR procedure, in comparison to the SFR method, seemed to render the best preserved hepatocytes.</description><identifier>ISSN: 0073-5655</identifier><identifier>EISSN: 2327-4328</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/bf02619627</identifier><identifier>PMID: 6151544</identifier><language>eng</language><publisher>Rockville, MD: Tissue Culture Association, Inc</publisher><subject>Animal cells ; Animals ; Biochemistry ; Biological and medical sciences ; Biotechnology ; Blood proteins ; Blood Proteins - biosynthesis ; Cell culture techniques ; Cell cultures. Hybridization. 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Jose Gomez-L.</creatorcontrib><creatorcontrib>Lopez, Pilar</creatorcontrib><creatorcontrib>Jose V. Castell</creatorcontrib><title>Biochemical Functionality and Recovery of Hepatocytes after Deep Freezing Storage</title><title>In Vitro</title><addtitle>In Vitro</addtitle><description>The present study was undertaken to define the conditions for optimal cryopreservation of hepatocytes. Two different freezing procedures were analyzed: a slow freezing rate (SFR) (-2° C/min down to -30° C and then quick freezing to -196° C) and a fast freezing rate (FFR) (direct freezing of tubes to -196° C: -39° C/min). Cells were frozen in fetal bovine serum containing 10% Dimethyl sulfoxide (DMSO). After rapid thawing at 37° C, followed by dilution and removal of the cryoprotectant, cells were plated and several parameters were followed as criteria for optimal cryopreservation of cells. The FFR cells showed no apparent ultrastructural damage after 24 h of culture. Plating efficiency and spreading were similar as controls. Gluconeogenesis from pyruvate and fructose, tyrosine amino transferase induction by glucagon and dexamethasone, urea production, and plasma protein synthesis of FFR cells were similar to those found in control cultures. The FFR procedure, in comparison to the SFR method, seemed to render the best preserved hepatocytes.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blood proteins</subject><subject>Blood Proteins - biosynthesis</subject><subject>Cell culture techniques</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cells, Cultured</subject><subject>Cryopreservation</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gluconeogenesis</subject><subject>Hepatocytes</subject><subject>Liver - cytology</subject><subject>Liver cells</subject><subject>Male</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular and cellular biology</subject><subject>Preservation, Biological</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Thawing</subject><subject>Tyrosine Transaminase - metabolism</subject><subject>Urea - biosynthesis</subject><subject>Viability</subject><issn>0073-5655</issn><issn>2327-4328</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LwzAchoMoc04vnhVyEA9CNR9NmhzddE4YiF_nkqa_zI6umUknzL_ejs2d3sPz8B4ehM4puaWEZHeFI0xSLVl2gPqMsyxJOVOHqN9BnggpxDE6iXFOCCeS0R7qSSqoSNM-eh1W3n7BorKmxuNVY9vKN6au2jU2TYnfwPofCGvsHZ7A0rTerluI2LgWAn4AWOJxAPitmhl-b30wMzhFR87UEc52O0Cf48eP0SSZvjw9j-6nieVatQl3RUaMMpKVBU1VqbSkYEtDLS9oVhCtNGNUUcIEyMKp0tGOaUKlItzJlA_Q9fZ3Gfz3CmKbL6pooa5NA34V80wokUqyEW-2og0-xgAuX4ZqYcI6pyTf9MuH4_9-nXy5e10VCyj36i5Yx6923MQumQumsVXca5pxyrTotIutNo9dlT1OmWZKE_4HPhZ_6g</recordid><startdate>198411</startdate><enddate>198411</enddate><creator>M. Jose Gomez-L.</creator><creator>Lopez, Pilar</creator><creator>Jose V. Castell</creator><general>Tissue Culture Association, Inc</general><general>Tissue Culture Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198411</creationdate><title>Biochemical Functionality and Recovery of Hepatocytes after Deep Freezing Storage</title><author>M. Jose Gomez-L. ; Lopez, Pilar ; Jose V. Castell</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-3fb70a8a62db148d8961ecda1c3b17b098922181025e6bf8df1a1c9016803f643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blood proteins</topic><topic>Blood Proteins - biosynthesis</topic><topic>Cell culture techniques</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>Cells, Cultured</topic><topic>Cryopreservation</topic><topic>Culture Media</topic><topic>Cultured cells</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gluconeogenesis</topic><topic>Hepatocytes</topic><topic>Liver - cytology</topic><topic>Liver cells</topic><topic>Male</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular and cellular biology</topic><topic>Preservation, Biological</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Thawing</topic><topic>Tyrosine Transaminase - metabolism</topic><topic>Urea - biosynthesis</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>M. Jose Gomez-L.</creatorcontrib><creatorcontrib>Lopez, Pilar</creatorcontrib><creatorcontrib>Jose V. 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Castell</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical Functionality and Recovery of Hepatocytes after Deep Freezing Storage</atitle><jtitle>In Vitro</jtitle><addtitle>In Vitro</addtitle><date>1984-11</date><risdate>1984</risdate><volume>20</volume><issue>11</issue><spage>826</spage><epage>832</epage><pages>826-832</pages><issn>0073-5655</issn><eissn>2327-4328</eissn><eissn>1475-2689</eissn><abstract>The present study was undertaken to define the conditions for optimal cryopreservation of hepatocytes. Two different freezing procedures were analyzed: a slow freezing rate (SFR) (-2° C/min down to -30° C and then quick freezing to -196° C) and a fast freezing rate (FFR) (direct freezing of tubes to -196° C: -39° C/min). Cells were frozen in fetal bovine serum containing 10% Dimethyl sulfoxide (DMSO). After rapid thawing at 37° C, followed by dilution and removal of the cryoprotectant, cells were plated and several parameters were followed as criteria for optimal cryopreservation of cells. The FFR cells showed no apparent ultrastructural damage after 24 h of culture. Plating efficiency and spreading were similar as controls. Gluconeogenesis from pyruvate and fructose, tyrosine amino transferase induction by glucagon and dexamethasone, urea production, and plasma protein synthesis of FFR cells were similar to those found in control cultures. The FFR procedure, in comparison to the SFR method, seemed to render the best preserved hepatocytes.</abstract><cop>Rockville, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>6151544</pmid><doi>10.1007/bf02619627</doi><tpages>7</tpages></addata></record> |
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| ispartof | In Vitro, 1984-11, Vol.20 (11), p.826-832 |
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| language | eng |
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| source | MEDLINE; SpringerNature Journals; JSTOR Archive Collection A-Z Listing |
| subjects | Animal cells Animals Biochemistry Biological and medical sciences Biotechnology Blood proteins Blood Proteins - biosynthesis Cell culture techniques Cell cultures. Hybridization. Fusion Cells, Cultured Cryopreservation Culture Media Cultured cells Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Freezing Fundamental and applied biological sciences. Psychology Gluconeogenesis Hepatocytes Liver - cytology Liver cells Male Methods. Procedures. Technologies Molecular and cellular biology Preservation, Biological Rats Rats, Inbred Strains Thawing Tyrosine Transaminase - metabolism Urea - biosynthesis Viability |
| title | Biochemical Functionality and Recovery of Hepatocytes after Deep Freezing Storage |
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