Neuron-Muscle Contact Changes Presynaptic Resting Calcium Set-Point
The role of retrograde signaling between target cells and their presynaptic partners during early cell-cell interactions was examined in cell culture. Using time-lapse video microscopy and fura-2 calcium analysis, we followed contacts between presynaptic neurites of Helisoma motoneurons and muscle f...
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| Veröffentlicht in: | Developmental biology 1993-07, Vol.158 (1), p.163-171 |
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| container_title | Developmental biology |
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| creator | Zoran, Mark J Funte, Lisa R Kater, SB Haydon, Philip G |
| description | The role of retrograde signaling between target cells and their presynaptic partners during early cell-cell interactions was examined in cell culture. Using time-lapse video microscopy and fura-2 calcium analysis, we followed contacts between presynaptic neurites of Helisoma motoneurons and muscle fibers dissociated from identified partners in chemical synaptogenesis. Cytosolic calcium rose dramatically at the region of cell-cell contact and subsequently increased throughout the entire neuron. Changes in the calcium set-point of presynaptic neurons were maintained even when connections with muscle targets were severed. Changes in presynaptic calcium concentration were not detected following contact with partners in electrical synapse formation. These data, taken together with the fact that calcium changes coincide with the time course of chemical synaptogenesis in these neuron-muscle cultures, suggest that calcium changes may be involved in early stages of synapse formation. |
| doi_str_mv | 10.1006/dbio.1993.1176 |
| format | Article |
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Using time-lapse video microscopy and fura-2 calcium analysis, we followed contacts between presynaptic neurites of Helisoma motoneurons and muscle fibers dissociated from identified partners in chemical synaptogenesis. Cytosolic calcium rose dramatically at the region of cell-cell contact and subsequently increased throughout the entire neuron. Changes in the calcium set-point of presynaptic neurons were maintained even when connections with muscle targets were severed. Changes in presynaptic calcium concentration were not detected following contact with partners in electrical synapse formation. These data, taken together with the fact that calcium changes coincide with the time course of chemical synaptogenesis in these neuron-muscle cultures, suggest that calcium changes may be involved in early stages of synapse formation.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1006/dbio.1993.1176</identifier><identifier>PMID: 8330670</identifier><identifier>CODEN: DEBIAO</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; Calcium - metabolism ; Cells, Cultured - metabolism ; Cytosol - metabolism ; Fundamental and applied biological sciences. Psychology ; Ganglia - embryology ; Invertebrates ; Mollusca ; Muscles - metabolism ; Neuromuscular Junction - metabolism ; Neurons - metabolism ; Physiology. 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Using time-lapse video microscopy and fura-2 calcium analysis, we followed contacts between presynaptic neurites of Helisoma motoneurons and muscle fibers dissociated from identified partners in chemical synaptogenesis. Cytosolic calcium rose dramatically at the region of cell-cell contact and subsequently increased throughout the entire neuron. Changes in the calcium set-point of presynaptic neurons were maintained even when connections with muscle targets were severed. Changes in presynaptic calcium concentration were not detected following contact with partners in electrical synapse formation. These data, taken together with the fact that calcium changes coincide with the time course of chemical synaptogenesis in these neuron-muscle cultures, suggest that calcium changes may be involved in early stages of synapse formation.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglia - embryology</subject><subject>Invertebrates</subject><subject>Mollusca</subject><subject>Muscles - metabolism</subject><subject>Neuromuscular Junction - metabolism</subject><subject>Neurons - metabolism</subject><subject>Physiology. 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Development</topic><topic>Snails - embryology</topic><topic>Synapses - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zoran, Mark J</creatorcontrib><creatorcontrib>Funte, Lisa R</creatorcontrib><creatorcontrib>Kater, SB</creatorcontrib><creatorcontrib>Haydon, Philip G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zoran, Mark J</au><au>Funte, Lisa R</au><au>Kater, SB</au><au>Haydon, Philip G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuron-Muscle Contact Changes Presynaptic Resting Calcium Set-Point</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>158</volume><issue>1</issue><spage>163</spage><epage>171</epage><pages>163-171</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><coden>DEBIAO</coden><abstract>The role of retrograde signaling between target cells and their presynaptic partners during early cell-cell interactions was examined in cell culture. Using time-lapse video microscopy and fura-2 calcium analysis, we followed contacts between presynaptic neurites of Helisoma motoneurons and muscle fibers dissociated from identified partners in chemical synaptogenesis. Cytosolic calcium rose dramatically at the region of cell-cell contact and subsequently increased throughout the entire neuron. Changes in the calcium set-point of presynaptic neurons were maintained even when connections with muscle targets were severed. Changes in presynaptic calcium concentration were not detected following contact with partners in electrical synapse formation. These data, taken together with the fact that calcium changes coincide with the time course of chemical synaptogenesis in these neuron-muscle cultures, suggest that calcium changes may be involved in early stages of synapse formation.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>8330670</pmid><doi>10.1006/dbio.1993.1176</doi><tpages>9</tpages></addata></record> |
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| ispartof | Developmental biology, 1993-07, Vol.158 (1), p.163-171 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biochemistry. Physiology. Immunology Biological and medical sciences Calcium - metabolism Cells, Cultured - metabolism Cytosol - metabolism Fundamental and applied biological sciences. Psychology Ganglia - embryology Invertebrates Mollusca Muscles - metabolism Neuromuscular Junction - metabolism Neurons - metabolism Physiology. Development Snails - embryology Synapses - metabolism |
| title | Neuron-Muscle Contact Changes Presynaptic Resting Calcium Set-Point |
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