Monoclonal antibodies to low density lipoprotein used for the study of low- and very-low-density lipoproteins, in “ELISA” and immunoprecipitation technics
Seven monoclonal antibodies to low-density lipoprotein were studied by the ELISA for their reactivity with LDL or VLDL. Cotitration experiments showed that five of them are addressed to different antigenic epitopes. Two of the monoclonal antibodies were temperature independent whereas the others had...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1984-12, Vol.125 (2), p.704-711 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Salmon, S. Goldstein, S. Pastier, D. Theron, L. Berthelier, M. Ayrault-Jarrier, M. Dubarry, M. Rebourcet, R. Pau, B. |
| description | Seven monoclonal antibodies to low-density lipoprotein were studied by the ELISA for their reactivity with LDL or VLDL. Cotitration experiments showed that five of them are addressed to different antigenic epitopes. Two of the monoclonal antibodies were temperature independent whereas the others had a decreased binding activity at 37°C compared to that obtained at 25°C or 4°C, suggesting the presence of antibodies directed to sequence or conformation epitopes, respectively. All antibodies reacted with both LDL and VLDL ; four of them had a higher affinity for LDL and two others for VLDL. Immunoprecipitation of LDL and/or VLDL was observed upon immunodiffusion with certain pairs of antibodies. This may allow the use of pairs of monoclonal antibodies to LDL for the quantitative determination of apolipoprotein B in serum LDL and VLDL. |
| doi_str_mv | 10.1016/0006-291X(84)90596-5 |
| format | Article |
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Cotitration experiments showed that five of them are addressed to different antigenic epitopes. Two of the monoclonal antibodies were temperature independent whereas the others had a decreased binding activity at 37°C compared to that obtained at 25°C or 4°C, suggesting the presence of antibodies directed to sequence or conformation epitopes, respectively. All antibodies reacted with both LDL and VLDL ; four of them had a higher affinity for LDL and two others for VLDL. Immunoprecipitation of LDL and/or VLDL was observed upon immunodiffusion with certain pairs of antibodies. This may allow the use of pairs of monoclonal antibodies to LDL for the quantitative determination of apolipoprotein B in serum LDL and VLDL.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(84)90596-5</identifier><identifier>PMID: 6083790</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Antibodies, Monoclonal ; Antigen-Antibody Complex ; antigenic determinants ; Applied sciences ; Enzyme-Linked Immunosorbent Assay ; Epitopes - analysis ; Exact sciences and technology ; Humans ; Immunodiffusion - methods ; Immunoenzyme Techniques ; immunoprecipitation ; lipoprotein (low density) ; Lipoproteins, LDL - blood ; Lipoproteins, LDL - immunology ; Lipoproteins, VLDL - blood ; Lipoproteins, VLDL - immunology ; monoclonal antibodies ; Other techniques and industries ; Temperature</subject><ispartof>Biochemical and biophysical research communications, 1984-12, Vol.125 (2), p.704-711</ispartof><rights>1984</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-7c1aba575df0c3be34d991adbc6ada964fa906d13daad2e60d8520ceae382e03</citedby><cites>FETCH-LOGICAL-c402t-7c1aba575df0c3be34d991adbc6ada964fa906d13daad2e60d8520ceae382e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(84)90596-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8833775$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6083790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salmon, S.</creatorcontrib><creatorcontrib>Goldstein, S.</creatorcontrib><creatorcontrib>Pastier, D.</creatorcontrib><creatorcontrib>Theron, L.</creatorcontrib><creatorcontrib>Berthelier, M.</creatorcontrib><creatorcontrib>Ayrault-Jarrier, M.</creatorcontrib><creatorcontrib>Dubarry, M.</creatorcontrib><creatorcontrib>Rebourcet, R.</creatorcontrib><creatorcontrib>Pau, B.</creatorcontrib><title>Monoclonal antibodies to low density lipoprotein used for the study of low- and very-low-density lipoproteins, in “ELISA” and immunoprecipitation technics</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Seven monoclonal antibodies to low-density lipoprotein were studied by the ELISA for their reactivity with LDL or VLDL. Cotitration experiments showed that five of them are addressed to different antigenic epitopes. Two of the monoclonal antibodies were temperature independent whereas the others had a decreased binding activity at 37°C compared to that obtained at 25°C or 4°C, suggesting the presence of antibodies directed to sequence or conformation epitopes, respectively. All antibodies reacted with both LDL and VLDL ; four of them had a higher affinity for LDL and two others for VLDL. Immunoprecipitation of LDL and/or VLDL was observed upon immunodiffusion with certain pairs of antibodies. This may allow the use of pairs of monoclonal antibodies to LDL for the quantitative determination of apolipoprotein B in serum LDL and VLDL.</description><subject>Antibodies, Monoclonal</subject><subject>Antigen-Antibody Complex</subject><subject>antigenic determinants</subject><subject>Applied sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - analysis</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Immunodiffusion - methods</subject><subject>Immunoenzyme Techniques</subject><subject>immunoprecipitation</subject><subject>lipoprotein (low density)</subject><subject>Lipoproteins, LDL - blood</subject><subject>Lipoproteins, LDL - immunology</subject><subject>Lipoproteins, VLDL - blood</subject><subject>Lipoproteins, VLDL - immunology</subject><subject>monoclonal antibodies</subject><subject>Other techniques and industries</subject><subject>Temperature</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EKtvCG4DkA0JFIjBOnMS-IFVVgUqLONADN8uxJ6pRYi-xU7S3PgjwcH0SnO5qTwhOo9F8_6_R_xPyjMEbBqx5CwBNUUr29VTwVxJq2RT1A7JiIKEoGfCHZHVAHpPjGL8BMMYbeUSOGhBVK2FFfn8KPpgheD1Q7ZPrgnUYaQp0CD-oRR9d2tLBbcJmCgmdp3NES_sw0XSNNKbZbmnoF7rIBpbe4LQtlu0v2viaZoO7258X68svZ3e3v-4VbhxnnxE0buOSTi54mtBce2fiE_Ko10PEp_t5Qq7eX1ydfyzWnz9cnp-tC8OhTEVrmO503da2B1N1WHErJdO2M422Wja81xIayyqrtS2xASvqEgxqrESJUJ2Qlzvb_Oj3GWNSo4sGh0F7DHNUbS24LFn5X5BxqEXOOIN8B5opxDhhrzaTG_W0VQzUUp9aulFLN0pwdV-fqrPs-d5_7ka0B9G-r3x_sb_raPTQT9obFw-YEFXVtovNux2GObMbh5OKxqE3aF2OOSkb3L__-APFwLzY</recordid><startdate>19841214</startdate><enddate>19841214</enddate><creator>Salmon, S.</creator><creator>Goldstein, S.</creator><creator>Pastier, D.</creator><creator>Theron, L.</creator><creator>Berthelier, M.</creator><creator>Ayrault-Jarrier, M.</creator><creator>Dubarry, M.</creator><creator>Rebourcet, R.</creator><creator>Pau, B.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19841214</creationdate><title>Monoclonal antibodies to low density lipoprotein used for the study of low- and very-low-density lipoproteins, in “ELISA” and immunoprecipitation technics</title><author>Salmon, S. ; Goldstein, S. ; Pastier, D. ; Theron, L. ; Berthelier, M. ; Ayrault-Jarrier, M. ; Dubarry, M. ; Rebourcet, R. ; Pau, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-7c1aba575df0c3be34d991adbc6ada964fa906d13daad2e60d8520ceae382e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Antibodies, Monoclonal</topic><topic>Antigen-Antibody Complex</topic><topic>antigenic determinants</topic><topic>Applied sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - analysis</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Immunodiffusion - methods</topic><topic>Immunoenzyme Techniques</topic><topic>immunoprecipitation</topic><topic>lipoprotein (low density)</topic><topic>Lipoproteins, LDL - blood</topic><topic>Lipoproteins, LDL - immunology</topic><topic>Lipoproteins, VLDL - blood</topic><topic>Lipoproteins, VLDL - immunology</topic><topic>monoclonal antibodies</topic><topic>Other techniques and industries</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salmon, S.</creatorcontrib><creatorcontrib>Goldstein, S.</creatorcontrib><creatorcontrib>Pastier, D.</creatorcontrib><creatorcontrib>Theron, L.</creatorcontrib><creatorcontrib>Berthelier, M.</creatorcontrib><creatorcontrib>Ayrault-Jarrier, M.</creatorcontrib><creatorcontrib>Dubarry, M.</creatorcontrib><creatorcontrib>Rebourcet, R.</creatorcontrib><creatorcontrib>Pau, B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salmon, S.</au><au>Goldstein, S.</au><au>Pastier, D.</au><au>Theron, L.</au><au>Berthelier, M.</au><au>Ayrault-Jarrier, M.</au><au>Dubarry, M.</au><au>Rebourcet, R.</au><au>Pau, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal antibodies to low density lipoprotein used for the study of low- and very-low-density lipoproteins, in “ELISA” and immunoprecipitation technics</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1984-12-14</date><risdate>1984</risdate><volume>125</volume><issue>2</issue><spage>704</spage><epage>711</epage><pages>704-711</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Seven monoclonal antibodies to low-density lipoprotein were studied by the ELISA for their reactivity with LDL or VLDL. Cotitration experiments showed that five of them are addressed to different antigenic epitopes. Two of the monoclonal antibodies were temperature independent whereas the others had a decreased binding activity at 37°C compared to that obtained at 25°C or 4°C, suggesting the presence of antibodies directed to sequence or conformation epitopes, respectively. All antibodies reacted with both LDL and VLDL ; four of them had a higher affinity for LDL and two others for VLDL. Immunoprecipitation of LDL and/or VLDL was observed upon immunodiffusion with certain pairs of antibodies. This may allow the use of pairs of monoclonal antibodies to LDL for the quantitative determination of apolipoprotein B in serum LDL and VLDL.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>6083790</pmid><doi>10.1016/0006-291X(84)90596-5</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1984-12, Vol.125 (2), p.704-711 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal Antigen-Antibody Complex antigenic determinants Applied sciences Enzyme-Linked Immunosorbent Assay Epitopes - analysis Exact sciences and technology Humans Immunodiffusion - methods Immunoenzyme Techniques immunoprecipitation lipoprotein (low density) Lipoproteins, LDL - blood Lipoproteins, LDL - immunology Lipoproteins, VLDL - blood Lipoproteins, VLDL - immunology monoclonal antibodies Other techniques and industries Temperature |
| title | Monoclonal antibodies to low density lipoprotein used for the study of low- and very-low-density lipoproteins, in “ELISA” and immunoprecipitation technics |
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