Nondeletional mechanisms of tolerance in total-lymphoid irradiation-induced bone marrow chimeras
To directly determine whether deletion mechanisms maintain tolerance after TLI, we applied the well-described negative selection phenomena of specific TCR bearing cells by Mls antigen to our TLI-prepared bone marrow chimeras (TLI-BMC)*. We chose DBA/2 mice as the host strain and CBA/CaJ as the donor...
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| Veröffentlicht in: | Transplantation 1993-07, Vol.56 (1), p.250-253 |
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| creator | FIELD, E. H STEINMULLER, D |
| description | To directly determine whether deletion mechanisms maintain tolerance after TLI, we applied the well-described negative selection phenomena of specific TCR bearing cells by Mls antigen to our TLI-prepared bone marrow chimeras (TLI-BMC)*. We chose DBA/2 mice as the host strain and CBA/CaJ as the donor strain because DBA/2 (Mls-1 super(a), Mls-2 super(a)) but not CBA/CaJ (Mls-1 super(b), Mls-2 super(b)) mice express Mls antigen and delete T cells that bear V beta 8.1 and V beta 3 T cell receptors. Staining of spleen cells in chimeras with monoclonal antibody against specific TCR determined the presence or absence of V beta 3 or V beta 8.1 T cells. If maintaining tolerance involves deletion mechanisms, then the percentage of V beta 3 and V beta 8.1 T cells in spleens from chimeras would resemble the V beta 3 and V beta 8.1 percentage from host DBA/2 mice. If the number of V beta 3 or V beta 8.1 cells resembles the CBA/CaJ host, then chimeras fail to delete potentially "autoreactive" T cells, and anergy or suppression mechanisms maintain tolerance. |
| doi_str_mv | 10.1097/00007890-199307000-00055 |
| format | Article |
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H ; STEINMULLER, D</creator><creatorcontrib>FIELD, E. H ; STEINMULLER, D</creatorcontrib><description>To directly determine whether deletion mechanisms maintain tolerance after TLI, we applied the well-described negative selection phenomena of specific TCR bearing cells by Mls antigen to our TLI-prepared bone marrow chimeras (TLI-BMC)*. We chose DBA/2 mice as the host strain and CBA/CaJ as the donor strain because DBA/2 (Mls-1 super(a), Mls-2 super(a)) but not CBA/CaJ (Mls-1 super(b), Mls-2 super(b)) mice express Mls antigen and delete T cells that bear V beta 8.1 and V beta 3 T cell receptors. Staining of spleen cells in chimeras with monoclonal antibody against specific TCR determined the presence or absence of V beta 3 or V beta 8.1 T cells. If maintaining tolerance involves deletion mechanisms, then the percentage of V beta 3 and V beta 8.1 T cells in spleens from chimeras would resemble the V beta 3 and V beta 8.1 percentage from host DBA/2 mice. If the number of V beta 3 or V beta 8.1 cells resembles the CBA/CaJ host, then chimeras fail to delete potentially "autoreactive" T cells, and anergy or suppression mechanisms maintain tolerance.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/00007890-199307000-00055</identifier><identifier>PMID: 8333061</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Biological and medical sciences ; Bone Marrow Transplantation ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Chimera ; Immunocompetence - drug effects ; Immunocompetence - radiation effects ; Immunosuppression - methods ; Interleukin-2 - pharmacology ; Lymphocyte Culture Test, Mixed ; Lymphocyte Depletion ; Medical sciences ; Mice ; Mice, Inbred CBA ; Mice, Inbred DBA ; Receptors, Antigen, T-Cell, alpha-beta - analysis ; Recombinant Proteins - pharmacology ; Spleen - immunology ; Spleen - radiation effects ; T-Lymphocytes - immunology ; T-Lymphocytes - radiation effects ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Whole-Body Irradiation</subject><ispartof>Transplantation, 1993-07, Vol.56 (1), p.250-253</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-3011bba8cc6979caaf2dfa6fa9a051ebc9a06404cb0515e2347087c477ab2c1a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3812211$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8333061$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FIELD, E. H</creatorcontrib><creatorcontrib>STEINMULLER, D</creatorcontrib><title>Nondeletional mechanisms of tolerance in total-lymphoid irradiation-induced bone marrow chimeras</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>To directly determine whether deletion mechanisms maintain tolerance after TLI, we applied the well-described negative selection phenomena of specific TCR bearing cells by Mls antigen to our TLI-prepared bone marrow chimeras (TLI-BMC)*. We chose DBA/2 mice as the host strain and CBA/CaJ as the donor strain because DBA/2 (Mls-1 super(a), Mls-2 super(a)) but not CBA/CaJ (Mls-1 super(b), Mls-2 super(b)) mice express Mls antigen and delete T cells that bear V beta 8.1 and V beta 3 T cell receptors. Staining of spleen cells in chimeras with monoclonal antibody against specific TCR determined the presence or absence of V beta 3 or V beta 8.1 T cells. If maintaining tolerance involves deletion mechanisms, then the percentage of V beta 3 and V beta 8.1 T cells in spleens from chimeras would resemble the V beta 3 and V beta 8.1 percentage from host DBA/2 mice. If the number of V beta 3 or V beta 8.1 cells resembles the CBA/CaJ host, then chimeras fail to delete potentially "autoreactive" T cells, and anergy or suppression mechanisms maintain tolerance.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Transplantation</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Chimera</subject><subject>Immunocompetence - drug effects</subject><subject>Immunocompetence - radiation effects</subject><subject>Immunosuppression - methods</subject><subject>Interleukin-2 - pharmacology</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Lymphocyte Depletion</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Mice, Inbred DBA</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - analysis</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Spleen - immunology</subject><subject>Spleen - radiation effects</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - radiation effects</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Whole-Body Irradiation</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9PAyEQxYnR1Fr9CCYcjDcUFnaBo2n8lzR60fM6y7Iphl0qbGP67aW29ioJmbzM7w1hHkKY0RtGtbyl-UilKWFacyqzIvmW5RGaspILUlFFj9GUUsEI41yeorOUPrcIl3KCJopzTis2RR8vYWitt6MLA3jcW7OEwaU-4dDhMXgbYTAWuyGLETzxm361DK7FLkZoHWx9xA3t2tgWN2GwuIcYwzc2S9dnczpHJx34ZC_2dYbeH-7f5k9k8fr4PL9bECMKOhJOGWsaUMZUWmoD0BVtB1UHGmjJbGNyrQQVpsmytAUXkipphJTQFIYBn6Hr3dxVDF9rm8a6d8lY72GwYZ1qWSqhpND_gqyqhC4qlUG1A00MKUXb1avo8u82NaP1NoX6L4X6kEL9m0K2Xu7fWDe9bQ_G_dpz_2rfh2TAd9slu3TAuGJFwRj_AQjbkLI</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>FIELD, E. H</creator><creator>STEINMULLER, D</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19930701</creationdate><title>Nondeletional mechanisms of tolerance in total-lymphoid irradiation-induced bone marrow chimeras</title><author>FIELD, E. H ; STEINMULLER, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-3011bba8cc6979caaf2dfa6fa9a051ebc9a06404cb0515e2347087c477ab2c1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Transplantation</topic><topic>Bone marrow, stem cells transplantation. Graft versus host reaction</topic><topic>Chimera</topic><topic>Immunocompetence - drug effects</topic><topic>Immunocompetence - radiation effects</topic><topic>Immunosuppression - methods</topic><topic>Interleukin-2 - pharmacology</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Lymphocyte Depletion</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>Mice, Inbred DBA</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - analysis</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Spleen - immunology</topic><topic>Spleen - radiation effects</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - radiation effects</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><topic>Whole-Body Irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FIELD, E. H</creatorcontrib><creatorcontrib>STEINMULLER, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FIELD, E. H</au><au>STEINMULLER, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nondeletional mechanisms of tolerance in total-lymphoid irradiation-induced bone marrow chimeras</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>56</volume><issue>1</issue><spage>250</spage><epage>253</epage><pages>250-253</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>To directly determine whether deletion mechanisms maintain tolerance after TLI, we applied the well-described negative selection phenomena of specific TCR bearing cells by Mls antigen to our TLI-prepared bone marrow chimeras (TLI-BMC)*. We chose DBA/2 mice as the host strain and CBA/CaJ as the donor strain because DBA/2 (Mls-1 super(a), Mls-2 super(a)) but not CBA/CaJ (Mls-1 super(b), Mls-2 super(b)) mice express Mls antigen and delete T cells that bear V beta 8.1 and V beta 3 T cell receptors. Staining of spleen cells in chimeras with monoclonal antibody against specific TCR determined the presence or absence of V beta 3 or V beta 8.1 T cells. If maintaining tolerance involves deletion mechanisms, then the percentage of V beta 3 and V beta 8.1 T cells in spleens from chimeras would resemble the V beta 3 and V beta 8.1 percentage from host DBA/2 mice. If the number of V beta 3 or V beta 8.1 cells resembles the CBA/CaJ host, then chimeras fail to delete potentially "autoreactive" T cells, and anergy or suppression mechanisms maintain tolerance.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>8333061</pmid><doi>10.1097/00007890-199307000-00055</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Biological and medical sciences Bone Marrow Transplantation Bone marrow, stem cells transplantation. Graft versus host reaction Chimera Immunocompetence - drug effects Immunocompetence - radiation effects Immunosuppression - methods Interleukin-2 - pharmacology Lymphocyte Culture Test, Mixed Lymphocyte Depletion Medical sciences Mice Mice, Inbred CBA Mice, Inbred DBA Receptors, Antigen, T-Cell, alpha-beta - analysis Recombinant Proteins - pharmacology Spleen - immunology Spleen - radiation effects T-Lymphocytes - immunology T-Lymphocytes - radiation effects Transfusions. Complications. Transfusion reactions. Cell and gene therapy Whole-Body Irradiation |
| title | Nondeletional mechanisms of tolerance in total-lymphoid irradiation-induced bone marrow chimeras |
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