A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries

Complete gas-liquid chromatographic separation of O-methyloxime acetates ( syn and anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single hydrolytic step for carbohydrate-containing biologi...

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Veröffentlicht in:	Analytical biochemistry 1984-10, Vol.142 (1), p.58-67
Hauptverfasser:	Neeser, Jean-Richard, Schweizer, Thomas F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Sugars - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences carbohydrate composition Carbohydrates Carbohydrates - analysis Chemical Phenomena Chemistry chromatography Chromatography, Gas fetuin Fundamental and applied biological sciences. Psychology gas-liquid chromatography Glycoproteins Hydrogen-Ion Concentration Hydrolysis mucin neutral and amino sugars O-methyloxime acetates Other biological molecules Oximes peroxidase Polysaccharides Temperature Trifluoroacetic Acid
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