A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries
Complete gas-liquid chromatographic separation of O-methyloxime acetates ( syn and anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single hydrolytic step for carbohydrate-containing biologi...
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| Veröffentlicht in: | Analytical biochemistry 1984-10, Vol.142 (1), p.58-67 |
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| container_title | Analytical biochemistry |
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| creator | Neeser, Jean-Richard Schweizer, Thomas F. |
| description | Complete gas-liquid chromatographic separation of
O-methyloxime acetates (
syn and
anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single hydrolytic step for carbohydrate-containing biological material (≤2.5· 10
−3
m sugar solution in 4
n trifluoroacetic acid at 125°C for 1h) has been developed, and results have been compared with those obtained with standard hydrolytic conditions in order to ensure complete release of amino sugars from glycoproteins, together with minimum losses of neutral sugars. The combination of this acid hydrolysis with the above improved derivatization procedure for the gas-liquid chromatographic analysis has led to a simple, rapid, and sensitive analytical method, which has been successfully tested on three glycoproteins (fetuin, mucin, and peroxidase) and two plant cell-wall polysaccharide fractions (soluble fibers from carrots and soybeans). |
| doi_str_mv | 10.1016/0003-2697(84)90516-5 |
| format | Article |
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O-methyloxime acetates (
syn and
anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single hydrolytic step for carbohydrate-containing biological material (≤2.5· 10
−3
m sugar solution in 4
n trifluoroacetic acid at 125°C for 1h) has been developed, and results have been compared with those obtained with standard hydrolytic conditions in order to ensure complete release of amino sugars from glycoproteins, together with minimum losses of neutral sugars. The combination of this acid hydrolysis with the above improved derivatization procedure for the gas-liquid chromatographic analysis has led to a simple, rapid, and sensitive analytical method, which has been successfully tested on three glycoproteins (fetuin, mucin, and peroxidase) and two plant cell-wall polysaccharide fractions (soluble fibers from carrots and soybeans).</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(84)90516-5</identifier><identifier>PMID: 6517320</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Sugars - analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; carbohydrate composition ; Carbohydrates ; Carbohydrates - analysis ; Chemical Phenomena ; Chemistry ; chromatography ; Chromatography, Gas ; fetuin ; Fundamental and applied biological sciences. Psychology ; gas-liquid chromatography ; Glycoproteins ; Hydrogen-Ion Concentration ; Hydrolysis ; mucin ; neutral and amino sugars ; O-methyloxime acetates ; Other biological molecules ; Oximes ; peroxidase ; Polysaccharides ; Temperature ; Trifluoroacetic Acid</subject><ispartof>Analytical biochemistry, 1984-10, Vol.142 (1), p.58-67</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-4db8107edcd98fc93aaf295dc602a138c9cc8da297e4e103614a4455ab7a08373</citedby><cites>FETCH-LOGICAL-c483t-4db8107edcd98fc93aaf295dc602a138c9cc8da297e4e103614a4455ab7a08373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(84)90516-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9168953$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6517320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neeser, Jean-Richard</creatorcontrib><creatorcontrib>Schweizer, Thomas F.</creatorcontrib><title>A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Complete gas-liquid chromatographic separation of
O-methyloxime acetates (
syn and
anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single hydrolytic step for carbohydrate-containing biological material (≤2.5· 10
−3
m sugar solution in 4
n trifluoroacetic acid at 125°C for 1h) has been developed, and results have been compared with those obtained with standard hydrolytic conditions in order to ensure complete release of amino sugars from glycoproteins, together with minimum losses of neutral sugars. The combination of this acid hydrolysis with the above improved derivatization procedure for the gas-liquid chromatographic analysis has led to a simple, rapid, and sensitive analytical method, which has been successfully tested on three glycoproteins (fetuin, mucin, and peroxidase) and two plant cell-wall polysaccharide fractions (soluble fibers from carrots and soybeans).</description><subject>Amino Sugars - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>carbohydrate composition</subject><subject>Carbohydrates</subject><subject>Carbohydrates - analysis</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>chromatography</subject><subject>Chromatography, Gas</subject><subject>fetuin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gas-liquid chromatography</subject><subject>Glycoproteins</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>mucin</subject><subject>neutral and amino sugars</subject><subject>O-methyloxime acetates</subject><subject>Other biological molecules</subject><subject>Oximes</subject><subject>peroxidase</subject><subject>Polysaccharides</subject><subject>Temperature</subject><subject>Trifluoroacetic Acid</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkt1u1DAQhSMEKqXwBiDNBUKtRMDeOIlzU6mq-JMq9QaurdnxZNcoibe2syIPzTvgZVe9hCv_zHfGR3NcFK-l-CCFbD4KIapy1XTtpVZXnahlU9ZPinMpuqYUleieFuePyPPiRYw_hZBS1c1ZcdbUsq1W4rz4fQMPM07JJUxuz2A5cRjdlE9-gvUChDs3DBgW2GAsB_cwOwu0DX7E5DcBd9sFfA8TzyngADhZwKz3EOcNhgiXGOG-HDltl8H_ciMDEufHOF69P9IQ02xzkwm2iw1-WJIjID9Zd_AQofcBNsNCfhd8YpdvDrJdBiMSbTE4yxHcBD5YDpB83lNgjHz0AIHJ7zk4ji-LZz0OkV-d1ovix-dP32-_lnf3X77d3tyVpHSVSmXXWoqWLdlO99RViP2qqy01YoWy0tQRaYurrmXFUlSNVKhUXeO6RaGrtroo3h37ZssPM8dkRheJ8xwn9nM0ba1VI7X8LyiVqHRONoPqCFLwMQbuzS64McdipDCH72AOWZtD1kYr8_c7mDrL3pz6z-uR7aPolH-uvz3VMRIOfcCJXHzEOtnorq4ydn3EOA9t7ziYSI4nYuvycJOx3v3bxx8uXtgd</recordid><startdate>198410</startdate><enddate>198410</enddate><creator>Neeser, Jean-Richard</creator><creator>Schweizer, Thomas F.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>198410</creationdate><title>A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries</title><author>Neeser, Jean-Richard ; Schweizer, Thomas F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-4db8107edcd98fc93aaf295dc602a138c9cc8da297e4e103614a4455ab7a08373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Amino Sugars - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>carbohydrate composition</topic><topic>Carbohydrates</topic><topic>Carbohydrates - analysis</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>chromatography</topic><topic>Chromatography, Gas</topic><topic>fetuin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gas-liquid chromatography</topic><topic>Glycoproteins</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>mucin</topic><topic>neutral and amino sugars</topic><topic>O-methyloxime acetates</topic><topic>Other biological molecules</topic><topic>Oximes</topic><topic>peroxidase</topic><topic>Polysaccharides</topic><topic>Temperature</topic><topic>Trifluoroacetic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neeser, Jean-Richard</creatorcontrib><creatorcontrib>Schweizer, Thomas F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neeser, Jean-Richard</au><au>Schweizer, Thomas F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1984-10</date><risdate>1984</risdate><volume>142</volume><issue>1</issue><spage>58</spage><epage>67</epage><pages>58-67</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>Complete gas-liquid chromatographic separation of
O-methyloxime acetates (
syn and
anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single hydrolytic step for carbohydrate-containing biological material (≤2.5· 10
−3
m sugar solution in 4
n trifluoroacetic acid at 125°C for 1h) has been developed, and results have been compared with those obtained with standard hydrolytic conditions in order to ensure complete release of amino sugars from glycoproteins, together with minimum losses of neutral sugars. The combination of this acid hydrolysis with the above improved derivatization procedure for the gas-liquid chromatographic analysis has led to a simple, rapid, and sensitive analytical method, which has been successfully tested on three glycoproteins (fetuin, mucin, and peroxidase) and two plant cell-wall polysaccharide fractions (soluble fibers from carrots and soybeans).</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>6517320</pmid><doi>10.1016/0003-2697(84)90516-5</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1984-10, Vol.142 (1), p.58-67 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75846181 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Sugars - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences carbohydrate composition Carbohydrates Carbohydrates - analysis Chemical Phenomena Chemistry chromatography Chromatography, Gas fetuin Fundamental and applied biological sciences. Psychology gas-liquid chromatography Glycoproteins Hydrogen-Ion Concentration Hydrolysis mucin neutral and amino sugars O-methyloxime acetates Other biological molecules Oximes peroxidase Polysaccharides Temperature Trifluoroacetic Acid |
| title | A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries |
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