Enzymatic and nonenzymatic degradation of myelin basic protein
A procedure for large scale isolation of myelin basic protein (BP) has been modified to insure BP preparations free of neutral proteinase activity. Fractions were monitored by electrophoretic analysis of BP solutions incubated under various conditions of temperature and pH. Maximum degradation of hu...
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| Veröffentlicht in: | Neurochemical research 1984-10, Vol.9 (10), p.1371-1385 |
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| container_title | Neurochemical research |
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| creator | DEIBLER, G. E BOYD, L. F KIES, M. W |
| description | A procedure for large scale isolation of myelin basic protein (BP) has been modified to insure BP preparations free of neutral proteinase activity. Fractions were monitored by electrophoretic analysis of BP solutions incubated under various conditions of temperature and pH. Maximum degradation of human BP prepared by the old batch procedure occurs at pH 7, approximately 47 degrees C. BP preparations obtained by the new procedure, as well as BP preparations purified by CM-cellulose chromatography, are stable under these conditions. The latter, however, do undergo significant breakdown at pH 9, 100 degrees C. The results suggest that the degradation observed under these conditions is non-enzymatic in nature. |
| doi_str_mv | 10.1007/BF00964664 |
| format | Article |
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E</creatorcontrib><creatorcontrib>BOYD, L. F</creatorcontrib><creatorcontrib>KIES, M. W</creatorcontrib><title>Enzymatic and nonenzymatic degradation of myelin basic protein</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>A procedure for large scale isolation of myelin basic protein (BP) has been modified to insure BP preparations free of neutral proteinase activity. Fractions were monitored by electrophoretic analysis of BP solutions incubated under various conditions of temperature and pH. Maximum degradation of human BP prepared by the old batch procedure occurs at pH 7, approximately 47 degrees C. BP preparations obtained by the new procedure, as well as BP preparations purified by CM-cellulose chromatography, are stable under these conditions. The latter, however, do undergo significant breakdown at pH 9, 100 degrees C. The results suggest that the degradation observed under these conditions is non-enzymatic in nature.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>central nervous system</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Lipoproteins, myelin</subject><subject>man</subject><subject>Methods</subject><subject>myelin basic protein</subject><subject>Myelin Basic Protein - isolation & purification</subject><subject>Myelin Sheath - analysis</subject><subject>Other techniques and industries</subject><subject>Peptide Hydrolases</subject><subject>Proteins</subject><subject>Temperature</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFLwzAUBvAgypzTi3ehB_EgVF_6kjS9CDo2FQZe9FySNJFKm2rTHeZfb3Rl3vSU8L4f78FHyCmFKwqQX98tAQrBhGB7ZEp5jqkoAPfJFFCwFGkBh-QohDeAyDM6IRMBEpngU3Kz8J-bVg21SZSvEt95uxtU9rVXVfx2Pulc0m5sU_tEqxCz974bbO2PyYFTTbAn4zsjL8vF8_whXT3dP85vV6nBnA6plhkvNM00E4VjDJwEwx3PpNNaxgyUBW00tYhYCTTa8TgXmGsuXAEZzsjFdm-8-7G2YSjbOhjbNMrbbh3KnEuGkuK_kDJAzmUR4eUWmr4LobeufO_rVvWbkkL53Wr522rEZ-PWtW5ttaNjjTE_H3MVjGpcr7ypw44VIDn9ufknkxKRRfYF2maJ3g</recordid><startdate>198410</startdate><enddate>198410</enddate><creator>DEIBLER, G. 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Psychology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Lipoproteins, myelin</topic><topic>man</topic><topic>Methods</topic><topic>myelin basic protein</topic><topic>Myelin Basic Protein - isolation & purification</topic><topic>Myelin Sheath - analysis</topic><topic>Other techniques and industries</topic><topic>Peptide Hydrolases</topic><topic>Proteins</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DEIBLER, G. E</creatorcontrib><creatorcontrib>BOYD, L. F</creatorcontrib><creatorcontrib>KIES, M. 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W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic and nonenzymatic degradation of myelin basic protein</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>1984-10</date><risdate>1984</risdate><volume>9</volume><issue>10</issue><spage>1371</spage><epage>1385</epage><pages>1371-1385</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>A procedure for large scale isolation of myelin basic protein (BP) has been modified to insure BP preparations free of neutral proteinase activity. Fractions were monitored by electrophoretic analysis of BP solutions incubated under various conditions of temperature and pH. Maximum degradation of human BP prepared by the old batch procedure occurs at pH 7, approximately 47 degrees C. BP preparations obtained by the new procedure, as well as BP preparations purified by CM-cellulose chromatography, are stable under these conditions. 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| ispartof | Neurochemical research, 1984-10, Vol.9 (10), p.1371-1385 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Analytical, structural and metabolic biochemistry Applied sciences Biological and medical sciences central nervous system Exact sciences and technology Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration Hydrolysis Lipoproteins, myelin man Methods myelin basic protein Myelin Basic Protein - isolation & purification Myelin Sheath - analysis Other techniques and industries Peptide Hydrolases Proteins Temperature |
| title | Enzymatic and nonenzymatic degradation of myelin basic protein |
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