Nitric Oxide Synthase Activity in the Pregnant Uterus Decreases at Parturition
The mechanisms that mediate changes in uterine activity from a quiescent state during pregnancy to active labor at parturition are unknown. Nitric oxide (NO), a potent mediator of smooth muscle relaxation, and its presence in the uterus is the subject of this report. Nitric oxide synthase (NOS) acti...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-07, Vol.194 (1), p.1-8 |
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| creator | Natuzzi, E.S. Ursell, P.C. Harrison, M. Buscher, C. Riemer, R.K. |
| description | The mechanisms that mediate changes in uterine activity from a quiescent state during pregnancy to active labor at parturition are unknown. Nitric oxide (NO), a potent mediator of smooth muscle relaxation, and its presence in the uterus is the subject of this report. Nitric oxide synthase (NOS) activity was demonstrated in nerves, blood vessels and decidua of gravid rat uterus by the NADPH-diaphorase staining method. Uterine tissue fixed during labor demonstrated markedly less NOS. Quantitation of NOS activity in subcellular fractions of pregnant and laboring uterus revealed its presence in both the cytosolic and the membranous compartments of uterine homogenates. In both cellular subfractions the enzyme activity decreased significantly from pregnancy to term. We conclude NOS is present in multiple structures within the uterus. Its presence in two cellular compartments suggests more than one form of NOS may be present in the uterus. Reduction in NOS activity at parturition suggests NO may contribute to the maintenance of uterine contractile quiescence during gestation. |
| doi_str_mv | 10.1006/bbrc.1993.1776 |
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Nitric oxide (NO), a potent mediator of smooth muscle relaxation, and its presence in the uterus is the subject of this report. Nitric oxide synthase (NOS) activity was demonstrated in nerves, blood vessels and decidua of gravid rat uterus by the NADPH-diaphorase staining method. Uterine tissue fixed during labor demonstrated markedly less NOS. Quantitation of NOS activity in subcellular fractions of pregnant and laboring uterus revealed its presence in both the cytosolic and the membranous compartments of uterine homogenates. In both cellular subfractions the enzyme activity decreased significantly from pregnancy to term. We conclude NOS is present in multiple structures within the uterus. Its presence in two cellular compartments suggests more than one form of NOS may be present in the uterus. Reduction in NOS activity at parturition suggests NO may contribute to the maintenance of uterine contractile quiescence during gestation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1776</identifier><identifier>PMID: 7687424</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Oxidoreductases - metabolism ; Animals ; Arterioles ; Biological and medical sciences ; Cell Membrane - enzymology ; Cytosol - enzymology ; Decidua - enzymology ; Endometrium - blood supply ; Endometrium - enzymology ; Endothelium, Vascular - enzymology ; Female ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; Labor, Obstetric - metabolism ; Myometrium - blood supply ; Myometrium - enzymology ; Myometrium - innervation ; Nerve Fibers - enzymology ; Nitric Oxide Synthase ; Postpartum Period - metabolism ; Pregnancy ; Pregnancy, Animal - metabolism ; Pregnancy. Parturition. Lactation ; Rats ; Rats, Inbred F344 ; Subcellular Fractions - enzymology ; Uterus - enzymology ; Vertebrates: reproduction</subject><ispartof>Biochemical and biophysical research communications, 1993-07, Vol.194 (1), p.1-8</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-b60e6d51f3e3da1f7a41e67ffd830966bc1c6788e31d89a9f005367a6a84c18f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1993.1776$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4865645$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7687424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Natuzzi, E.S.</creatorcontrib><creatorcontrib>Ursell, P.C.</creatorcontrib><creatorcontrib>Harrison, M.</creatorcontrib><creatorcontrib>Buscher, C.</creatorcontrib><creatorcontrib>Riemer, R.K.</creatorcontrib><title>Nitric Oxide Synthase Activity in the Pregnant Uterus Decreases at Parturition</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The mechanisms that mediate changes in uterine activity from a quiescent state during pregnancy to active labor at parturition are unknown. Nitric oxide (NO), a potent mediator of smooth muscle relaxation, and its presence in the uterus is the subject of this report. Nitric oxide synthase (NOS) activity was demonstrated in nerves, blood vessels and decidua of gravid rat uterus by the NADPH-diaphorase staining method. Uterine tissue fixed during labor demonstrated markedly less NOS. Quantitation of NOS activity in subcellular fractions of pregnant and laboring uterus revealed its presence in both the cytosolic and the membranous compartments of uterine homogenates. In both cellular subfractions the enzyme activity decreased significantly from pregnancy to term. We conclude NOS is present in multiple structures within the uterus. Its presence in two cellular compartments suggests more than one form of NOS may be present in the uterus. Reduction in NOS activity at parturition suggests NO may contribute to the maintenance of uterine contractile quiescence during gestation.</description><subject>Amino Acid Oxidoreductases - metabolism</subject><subject>Animals</subject><subject>Arterioles</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - enzymology</subject><subject>Cytosol - enzymology</subject><subject>Decidua - enzymology</subject><subject>Endometrium - blood supply</subject><subject>Endometrium - enzymology</subject><subject>Endothelium, Vascular - enzymology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Labor, Obstetric - metabolism</subject><subject>Myometrium - blood supply</subject><subject>Myometrium - enzymology</subject><subject>Myometrium - innervation</subject><subject>Nerve Fibers - enzymology</subject><subject>Nitric Oxide Synthase</subject><subject>Postpartum Period - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy, Animal - metabolism</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Subcellular Fractions - enzymology</subject><subject>Uterus - enzymology</subject><subject>Vertebrates: reproduction</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10L9LAzEUwPEgSq3V1U3IIG5XkyaX5EbxN5S2oAW3kMu92Eh7p0mu2P_eKy3dnDK8z3uEL0KXlAwpIeK2LIMd0qJgQyqlOEJ9SgqSjSjhx6hPOpGNCvpxis5i_CKEUi6KHupJoSQf8T6aTHwK3uLpr68Av23qtDAR8J1Nfu3TBvsapwXgWYDP2tQJzxOENuIHsAE6GLFJeGZCaoNPvqnP0YkzywgX-3eA5k-P7_cv2Xj6_Hp_N84sZzxlpSAgqpw6Bqwy1EnDKQjpXKUYKYQoLbVCKgWMVqowhSMkZ0IaYRS3VDk2QDe7u9-h-WkhJr3y0cJyaWpo2qhlrjgbKdLB4Q7a0MQYwOnv4FcmbDQlehtQbwPqbUC9DdgtXO0vt-UKqgPfF-vm1_u5idYsXTC19fHAuBK54HnH1I5BV2HtIehoPdQWKh_AJl01_r8f_AE94owc</recordid><startdate>19930715</startdate><enddate>19930715</enddate><creator>Natuzzi, E.S.</creator><creator>Ursell, P.C.</creator><creator>Harrison, M.</creator><creator>Buscher, C.</creator><creator>Riemer, R.K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930715</creationdate><title>Nitric Oxide Synthase Activity in the Pregnant Uterus Decreases at Parturition</title><author>Natuzzi, E.S. ; Ursell, P.C. ; Harrison, M. ; Buscher, C. ; Riemer, R.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-b60e6d51f3e3da1f7a41e67ffd830966bc1c6788e31d89a9f005367a6a84c18f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Oxidoreductases - metabolism</topic><topic>Animals</topic><topic>Arterioles</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - enzymology</topic><topic>Cytosol - enzymology</topic><topic>Decidua - enzymology</topic><topic>Endometrium - blood supply</topic><topic>Endometrium - enzymology</topic><topic>Endothelium, Vascular - enzymology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Labor, Obstetric - metabolism</topic><topic>Myometrium - blood supply</topic><topic>Myometrium - enzymology</topic><topic>Myometrium - innervation</topic><topic>Nerve Fibers - enzymology</topic><topic>Nitric Oxide Synthase</topic><topic>Postpartum Period - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy, Animal - metabolism</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Subcellular Fractions - enzymology</topic><topic>Uterus - enzymology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Natuzzi, E.S.</creatorcontrib><creatorcontrib>Ursell, P.C.</creatorcontrib><creatorcontrib>Harrison, M.</creatorcontrib><creatorcontrib>Buscher, C.</creatorcontrib><creatorcontrib>Riemer, R.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Natuzzi, E.S.</au><au>Ursell, P.C.</au><au>Harrison, M.</au><au>Buscher, C.</au><au>Riemer, R.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric Oxide Synthase Activity in the Pregnant Uterus Decreases at Parturition</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-07-15</date><risdate>1993</risdate><volume>194</volume><issue>1</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The mechanisms that mediate changes in uterine activity from a quiescent state during pregnancy to active labor at parturition are unknown. Nitric oxide (NO), a potent mediator of smooth muscle relaxation, and its presence in the uterus is the subject of this report. Nitric oxide synthase (NOS) activity was demonstrated in nerves, blood vessels and decidua of gravid rat uterus by the NADPH-diaphorase staining method. Uterine tissue fixed during labor demonstrated markedly less NOS. Quantitation of NOS activity in subcellular fractions of pregnant and laboring uterus revealed its presence in both the cytosolic and the membranous compartments of uterine homogenates. In both cellular subfractions the enzyme activity decreased significantly from pregnancy to term. We conclude NOS is present in multiple structures within the uterus. Its presence in two cellular compartments suggests more than one form of NOS may be present in the uterus. Reduction in NOS activity at parturition suggests NO may contribute to the maintenance of uterine contractile quiescence during gestation.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7687424</pmid><doi>10.1006/bbrc.1993.1776</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Oxidoreductases - metabolism Animals Arterioles Biological and medical sciences Cell Membrane - enzymology Cytosol - enzymology Decidua - enzymology Endometrium - blood supply Endometrium - enzymology Endothelium, Vascular - enzymology Female Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Labor, Obstetric - metabolism Myometrium - blood supply Myometrium - enzymology Myometrium - innervation Nerve Fibers - enzymology Nitric Oxide Synthase Postpartum Period - metabolism Pregnancy Pregnancy, Animal - metabolism Pregnancy. Parturition. Lactation Rats Rats, Inbred F344 Subcellular Fractions - enzymology Uterus - enzymology Vertebrates: reproduction |
| title | Nitric Oxide Synthase Activity in the Pregnant Uterus Decreases at Parturition |
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