A method for isolation of cDNA-quality mRNA from immature seeds of a gymnosperm rich in polyphenolics
Gymnosperms accumulate many secondary metabolites including bisflavonoids, mono- and diterpenes, etheric oils, resins, cyclitols, shikimic acid and lignins. After several failures with methods suitable for most plants, we were finally able to prepare quite pure poly(A) super(+) mRNA from whole seeds...
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| Veröffentlicht in: | Plant molecular biology 1993-06, Vol.22 (3), p.555-556 |
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| container_title | Plant molecular biology |
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| creator | Martin, W. (Technische Univ. Braunschweig (Germany). Inst. fuer Genetik) Nock, S Meyer-Gauen, G Haeger, K.P Jensen, U Cerff, R |
| description | Gymnosperms accumulate many secondary metabolites including bisflavonoids, mono- and diterpenes, etheric oils, resins, cyclitols, shikimic acid and lignins. After several failures with methods suitable for most plants, we were finally able to prepare quite pure poly(A) super(+) mRNA from whole seeds of Taxus baccata (English yew). This tissue is partially lignified and contains very high levels of phenolic compounds. The cDNA synthesis directed by this mRNA was very satisfactory. For the benefit of those working with similarly "difficult" plant tissues, we describe here the method of mRNA isolation which we improvised through modification of a previously published procedure. |
| doi_str_mv | 10.1007/BF00015986 |
| format | Article |
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(Technische Univ. Braunschweig (Germany). Inst. fuer Genetik)</creatorcontrib><creatorcontrib>Nock, S</creatorcontrib><creatorcontrib>Meyer-Gauen, G</creatorcontrib><creatorcontrib>Haeger, K.P</creatorcontrib><creatorcontrib>Jensen, U</creatorcontrib><creatorcontrib>Cerff, R</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martin, W. (Technische Univ. Braunschweig (Germany). 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| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1993-06, Vol.22 (3), p.555-556 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | ADN ARN MENSAJERO ARN MESSAGER CLONACION MOLECULAR CLONAGE MOLECULAIRE COMPOSE PHENOLIQUE COMPUESTOS FENOLICOS DNA Flavonoids Genetic Techniques GRAINE MESSENGER RNA METABOLITE METABOLITES METABOLITOS METHODE METHODS METODOS MOLECULAR CLONING PHENOLIC COMPOUNDS Phenols Plants - genetics Polymers Polyphenols RNA, Messenger - isolation & purification SEEDS SEMILLA TAXUS BACCATA |
| title | A method for isolation of cDNA-quality mRNA from immature seeds of a gymnosperm rich in polyphenolics |
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