Mouse c-mos Oncogene Activation is Prevented by Upstream Sequences
Although the molecularly cloned mouse c-mos oncogene locus can be efficiently activated by insertion of a retroviral long terminal repeat (LTR) 5′to its coding region, only low-frequency transformation occurs with the LTR element inserted 3′to this region. Analysis of several of the latter transform...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1984-12, Vol.81 (24), p.7817-7821 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 81 |
| creator | Wood, T. G. McGeady, M. L. Baroudy, B. M. Blair, D. G. G. F. Vande Woude |
| description | Although the molecularly cloned mouse c-mos oncogene locus can be efficiently activated by insertion of a retroviral long terminal repeat (LTR) 5′to its coding region, only low-frequency transformation occurs with the LTR element inserted 3′to this region. Analysis of several of the latter transformed cell lines suggested that loss of 2 kilobases (kb) of normal mouse DNA sequences preceding c-mos was required for oncogene activation. The determination of the transforming potential of deletion mutants containing only portions of this region followed by analysis of their nucleotide sequences identified a region termed upstream mouse sequence (UMS) as a cis-acting locus that prevents c-mos activation by a 3′LTR. The UMS region is ≈ 1 kb in length and is located 0.8-1.8 kb upstream from the first ATG in the open reading frame of c-mos. Insertion of UMS 5′to the v-mos coding region also prevents 3′LTR enhancement of its transforming activity, but this inhibition is position dependent and functions only when inserted between v-mos and its putative promoter. The results presented here suggest that UMS may function to regulate c-mos proto-oncogene expression and may explain the lack of detectable c-mos transcripts in normal mouse cells. |
| doi_str_mv | 10.1073/pnas.81.24.7817 |
| format | Article |
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G. ; McGeady, M. L. ; Baroudy, B. M. ; Blair, D. G. ; G. F. Vande Woude</creator><creatorcontrib>Wood, T. G. ; McGeady, M. L. ; Baroudy, B. M. ; Blair, D. G. ; G. F. Vande Woude</creatorcontrib><description>Although the molecularly cloned mouse c-mos oncogene locus can be efficiently activated by insertion of a retroviral long terminal repeat (LTR) 5′to its coding region, only low-frequency transformation occurs with the LTR element inserted 3′to this region. Analysis of several of the latter transformed cell lines suggested that loss of 2 kilobases (kb) of normal mouse DNA sequences preceding c-mos was required for oncogene activation. The determination of the transforming potential of deletion mutants containing only portions of this region followed by analysis of their nucleotide sequences identified a region termed upstream mouse sequence (UMS) as a cis-acting locus that prevents c-mos activation by a 3′LTR. The UMS region is ≈ 1 kb in length and is located 0.8-1.8 kb upstream from the first ATG in the open reading frame of c-mos. Insertion of UMS 5′to the v-mos coding region also prevents 3′LTR enhancement of its transforming activity, but this inhibition is position dependent and functions only when inserted between v-mos and its putative promoter. The results presented here suggest that UMS may function to regulate c-mos proto-oncogene expression and may explain the lack of detectable c-mos transcripts in normal mouse cells.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.81.24.7817</identifier><identifier>PMID: 6096859</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Base Sequence ; Cell Transformation, Neoplastic ; Cells, Cultured ; Cloning, Molecular ; DNA ; DNA probes ; DNA Restriction Enzymes ; Gene Expression Regulation ; Genetic loci ; Mice ; Mice, Inbred Strains ; Moloney murine sarcoma virus - genetics ; NIH 3T3 cells ; Nucleotide sequences ; Oncogenes ; Open reading frames ; Plasmids ; RNA ; Transfection</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1984-12, Vol.81 (24), p.7817-7821</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-9f07a01a7c48ddf9b1e05d01a51df8a551a91d1b7c23ba867f9c54c6bcd42053</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/81/24.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24431$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24431$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6096859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wood, T. G.</creatorcontrib><creatorcontrib>McGeady, M. L.</creatorcontrib><creatorcontrib>Baroudy, B. M.</creatorcontrib><creatorcontrib>Blair, D. G.</creatorcontrib><creatorcontrib>G. F. Vande Woude</creatorcontrib><title>Mouse c-mos Oncogene Activation is Prevented by Upstream Sequences</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Although the molecularly cloned mouse c-mos oncogene locus can be efficiently activated by insertion of a retroviral long terminal repeat (LTR) 5′to its coding region, only low-frequency transformation occurs with the LTR element inserted 3′to this region. Analysis of several of the latter transformed cell lines suggested that loss of 2 kilobases (kb) of normal mouse DNA sequences preceding c-mos was required for oncogene activation. The determination of the transforming potential of deletion mutants containing only portions of this region followed by analysis of their nucleotide sequences identified a region termed upstream mouse sequence (UMS) as a cis-acting locus that prevents c-mos activation by a 3′LTR. The UMS region is ≈ 1 kb in length and is located 0.8-1.8 kb upstream from the first ATG in the open reading frame of c-mos. Insertion of UMS 5′to the v-mos coding region also prevents 3′LTR enhancement of its transforming activity, but this inhibition is position dependent and functions only when inserted between v-mos and its putative promoter. The results presented here suggest that UMS may function to regulate c-mos proto-oncogene expression and may explain the lack of detectable c-mos transcripts in normal mouse cells.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA Restriction Enzymes</subject><subject>Gene Expression Regulation</subject><subject>Genetic loci</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Moloney murine sarcoma virus - genetics</subject><subject>NIH 3T3 cells</subject><subject>Nucleotide sequences</subject><subject>Oncogenes</subject><subject>Open reading frames</subject><subject>Plasmids</subject><subject>RNA</subject><subject>Transfection</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP3DAQha2qFWxpz5UqUeXUnrKMHTu2Dz0AaikSFZWgZ8txJhCUxFvbu4J_X692WcqFnkaa973Rsx8hHyjMKcjqaDHZOFd0zvhcKipfkRkFTcuaa3hNZgBMloozvk_exngHAFoo2CN7NehaCT0jJz_9MmLhytHH4nJy_gYnLI5d6lc29X4q-lj8CrjCKWFbNA_F70VMAe1YXOGfJU4O4zvyprNDxPfbeUCuv3-7Pv1RXlyenZ8eX5SOa5ZK3YG0QK10XLVtpxuKINq8ELTtlBWCWk1b2kjHqsaqWnbaCe7qxrWcgagOyNfN2cWyGbF1OVGwg1mEfrThwXjbm-fK1N-aG78ylWaMV9n_eesPPiePyYx9dDgMdsL8B0YKVTHO6H9ByrlgoHgGjzagCz7GgN0uDAWzbses2zGKGsbNup3sOPz3DTt-W0fWv2z1tfFRfTpguuUwJLxPmfz0IpmBjxvgLiYfdgTjvKLVX0bZrWA</recordid><startdate>19841201</startdate><enddate>19841201</enddate><creator>Wood, T. G.</creator><creator>McGeady, M. L.</creator><creator>Baroudy, B. M.</creator><creator>Blair, D. G.</creator><creator>G. F. Vande Woude</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19841201</creationdate><title>Mouse c-mos Oncogene Activation is Prevented by Upstream Sequences</title><author>Wood, T. G. ; McGeady, M. L. ; Baroudy, B. M. ; Blair, D. G. ; G. F. Vande Woude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-9f07a01a7c48ddf9b1e05d01a51df8a551a91d1b7c23ba867f9c54c6bcd42053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA probes</topic><topic>DNA Restriction Enzymes</topic><topic>Gene Expression Regulation</topic><topic>Genetic loci</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Moloney murine sarcoma virus - genetics</topic><topic>NIH 3T3 cells</topic><topic>Nucleotide sequences</topic><topic>Oncogenes</topic><topic>Open reading frames</topic><topic>Plasmids</topic><topic>RNA</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wood, T. G.</creatorcontrib><creatorcontrib>McGeady, M. L.</creatorcontrib><creatorcontrib>Baroudy, B. M.</creatorcontrib><creatorcontrib>Blair, D. G.</creatorcontrib><creatorcontrib>G. F. 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Vande Woude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mouse c-mos Oncogene Activation is Prevented by Upstream Sequences</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1984-12-01</date><risdate>1984</risdate><volume>81</volume><issue>24</issue><spage>7817</spage><epage>7821</epage><pages>7817-7821</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Although the molecularly cloned mouse c-mos oncogene locus can be efficiently activated by insertion of a retroviral long terminal repeat (LTR) 5′to its coding region, only low-frequency transformation occurs with the LTR element inserted 3′to this region. Analysis of several of the latter transformed cell lines suggested that loss of 2 kilobases (kb) of normal mouse DNA sequences preceding c-mos was required for oncogene activation. The determination of the transforming potential of deletion mutants containing only portions of this region followed by analysis of their nucleotide sequences identified a region termed upstream mouse sequence (UMS) as a cis-acting locus that prevents c-mos activation by a 3′LTR. The UMS region is ≈ 1 kb in length and is located 0.8-1.8 kb upstream from the first ATG in the open reading frame of c-mos. Insertion of UMS 5′to the v-mos coding region also prevents 3′LTR enhancement of its transforming activity, but this inhibition is position dependent and functions only when inserted between v-mos and its putative promoter. The results presented here suggest that UMS may function to regulate c-mos proto-oncogene expression and may explain the lack of detectable c-mos transcripts in normal mouse cells.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6096859</pmid><doi>10.1073/pnas.81.24.7817</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Base Sequence Cell Transformation, Neoplastic Cells, Cultured Cloning, Molecular DNA DNA probes DNA Restriction Enzymes Gene Expression Regulation Genetic loci Mice Mice, Inbred Strains Moloney murine sarcoma virus - genetics NIH 3T3 cells Nucleotide sequences Oncogenes Open reading frames Plasmids RNA Transfection |
| title | Mouse c-mos Oncogene Activation is Prevented by Upstream Sequences |
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