Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution
The cDNA for Endothelin-2 (ET-2) has been previously cloned and characterised; however, ET-2 remains the least studied of the endothelin isopeptides and little is known of its function and location. In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven a...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-06, Vol.193 (3), p.834-840 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Oreilly, G. Charnockjones, D.S. Morrison, J.J. Cameron, I.T. Davenport, A.P. Smith, S.K. |
| description | The cDNA for Endothelin-2 (ET-2) has been previously cloned and characterised; however, ET-2 remains the least studied of the endothelin isopeptides and little is known of its function and location. In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants. |
| doi_str_mv | 10.1006/bbrc.1993.1701 |
| format | Article |
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In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1701</identifier><identifier>PMID: 8323558</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Alternative Splicing ; amino acid sequence ; Amnion - metabolism ; Base Sequence ; Biological and medical sciences ; cDNA ; Chorion - metabolism ; Cloning, Molecular ; Electrophoresis, Agar Gel ; Endometrium - metabolism ; endothelin 2 ; Endothelins - biosynthesis ; Endothelins - genetics ; Exons ; Female ; Fundamental and applied biological sciences. Psychology ; genes ; Genes. Genome ; Genetic Variation ; Humans ; man ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; mRNA ; Myocardium - metabolism ; Myometrium - metabolism ; nucleotide sequence ; Oligodeoxyribonucleotides ; Organ Specificity ; Placenta - metabolism ; Polymerase Chain Reaction ; predictions ; Pregnancy ; RNA, Messenger - isolation & purification ; RNA, Messenger - metabolism ; Sequence Homology, Nucleic Acid ; splicing</subject><ispartof>Biochemical and biophysical research communications, 1993-06, Vol.193 (3), p.834-840</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-5998d906e25542196224b14c2f42a3f9e0ac99ee59ef57e084812e272465a5a23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X83717018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4825388$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8323558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oreilly, G.</creatorcontrib><creatorcontrib>Charnockjones, D.S.</creatorcontrib><creatorcontrib>Morrison, J.J.</creatorcontrib><creatorcontrib>Cameron, I.T.</creatorcontrib><creatorcontrib>Davenport, A.P.</creatorcontrib><creatorcontrib>Smith, S.K.</creatorcontrib><title>Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The cDNA for Endothelin-2 (ET-2) has been previously cloned and characterised; however, ET-2 remains the least studied of the endothelin isopeptides and little is known of its function and location. In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants.</description><subject>Alternative Splicing</subject><subject>amino acid sequence</subject><subject>Amnion - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA</subject><subject>Chorion - metabolism</subject><subject>Cloning, Molecular</subject><subject>Electrophoresis, Agar Gel</subject><subject>Endometrium - metabolism</subject><subject>endothelin 2</subject><subject>Endothelins - biosynthesis</subject><subject>Endothelins - genetics</subject><subject>Exons</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genes. Genome</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>man</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>mRNA</subject><subject>Myocardium - metabolism</subject><subject>Myometrium - metabolism</subject><subject>nucleotide sequence</subject><subject>Oligodeoxyribonucleotides</subject><subject>Organ Specificity</subject><subject>Placenta - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>predictions</subject><subject>Pregnancy</subject><subject>RNA, Messenger - isolation & purification</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>splicing</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFr2zAUh8VYadNu190KOozdnOnJkiMdQ5YthbCWLYPdhCw_ExVbTiW70P--Ngm5lZ7e4fe9H4_vEfIF2BwYK76XZXRz0Dqfw4LBBzIDplnGgYmPZMZGIuMa_l-R65QeGQMQhb4klyrnuZRqRh6WTY8x2N4_Y_NC_x4a77Ci7Z_fy0TrLtLN0NpA16Hq-j02PmSc2lDR3R59pDuf0oD0h0999OXQ-y58Ihe1bRJ-Ps0b8u_nerfaZNv7X3er5TZzOYg-k1qrSrMCuZSCgy44FyUIx2vBbV5rZNZpjSg11nKBTAkFHPmCi0JaaXl-Q74dew-xexow9ab1yWHT2IDdkMxCKl4IAe-CUBRSi3wC50fQxS6liLU5RN_a-GKAmcm1mVybybWZXI8Lt6fmoWyxOuMnuWP-9ZTb5GxTRxucT2dMKC5zNWHqiOGo69ljNMl5DOMbfETXm6rzb13wCi_UmGA</recordid><startdate>19930630</startdate><enddate>19930630</enddate><creator>Oreilly, G.</creator><creator>Charnockjones, D.S.</creator><creator>Morrison, J.J.</creator><creator>Cameron, I.T.</creator><creator>Davenport, A.P.</creator><creator>Smith, S.K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19930630</creationdate><title>Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution</title><author>Oreilly, G. ; Charnockjones, D.S. ; Morrison, J.J. ; Cameron, I.T. ; Davenport, A.P. ; Smith, S.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-5998d906e25542196224b14c2f42a3f9e0ac99ee59ef57e084812e272465a5a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Alternative Splicing</topic><topic>amino acid sequence</topic><topic>Amnion - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cDNA</topic><topic>Chorion - metabolism</topic><topic>Cloning, Molecular</topic><topic>Electrophoresis, Agar Gel</topic><topic>Endometrium - metabolism</topic><topic>endothelin 2</topic><topic>Endothelins - biosynthesis</topic><topic>Endothelins - genetics</topic><topic>Exons</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genes. 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In the present study reverse transcriptase-polymerase chain reaction revealed the presence of seven alternatively spliced mRNA variants encoding ET-2, with a specific pattern of distribution in various human tissues. Computer alignment and analysis of the DNA sequences demonstrated alternative splicing of five exons of 52, 169, 123, 99 and 174 base pairs, in the carboxy terminal region of the mRNA encoding preproET-2. This region contains sites for the post-transcriptional processing of preproET-2 into mature ET-2, therefore we postulate that post-transcriptional processing may be disrupted or altered in these variants.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8323558</pmid><doi>10.1006/bbrc.1993.1701</doi><tpages>7</tpages></addata></record> |
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| subjects | Alternative Splicing amino acid sequence Amnion - metabolism Base Sequence Biological and medical sciences cDNA Chorion - metabolism Cloning, Molecular Electrophoresis, Agar Gel Endometrium - metabolism endothelin 2 Endothelins - biosynthesis Endothelins - genetics Exons Female Fundamental and applied biological sciences. Psychology genes Genes. Genome Genetic Variation Humans man Molecular and cellular biology Molecular genetics Molecular Sequence Data mRNA Myocardium - metabolism Myometrium - metabolism nucleotide sequence Oligodeoxyribonucleotides Organ Specificity Placenta - metabolism Polymerase Chain Reaction predictions Pregnancy RNA, Messenger - isolation & purification RNA, Messenger - metabolism Sequence Homology, Nucleic Acid splicing |
| title | Alternatively Spliced mRNAs for Human Endothelin-2 and Their Tissue Distribution |
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