The effects of growth factors and serum on DNA synthesis and differentiation in thyroid cells in culture
The effects of three putative growth factors and serum on [ Me- 3H]thymidine and Na 125I incorporation into thyroid cell cultures have been examined. We found that serum and EGF could stimulate radioactively labelled thymidine incorporation into confluent cultures. However, both factors completely i...
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| Veröffentlicht in: | Molecular and cellular endocrinology 1984-12, Vol.38 (2), p.141-150 |
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| container_title | Molecular and cellular endocrinology |
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| creator | Eggo, M.C. Bachrach, L.K. Fayet, G. Errick, J. Kudlow, J.E. Cohen, M.F. Burrow, G.N. |
| description | The effects of three putative growth factors and serum on [
Me-
3H]thymidine and Na
125I incorporation into thyroid cell cultures have been examined. We found that serum and EGF could stimulate radioactively labelled thymidine incorporation into confluent cultures. However, both factors completely inhibited iodine uptake and organification at low concentrations. Insulin also stimulated [
Me-
3H]thymidine incorporation but had no adverse effect on thyroid differentiated function. TSH examined under the same conditions was not a growth factor but was essential to maintain differentiated functions. We conclude that thyroid growth and differentiation are not mutually exclusive processes. However, EGF and serum inhibit thyroid differentiated function at very low concentrations. Elucidation of the physiological role of these factors and their mechanism of action may lead to a greater understanding of thyroid hormone biosynthesis. |
| doi_str_mv | 10.1016/0303-7207(84)90112-6 |
| format | Article |
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Me-
3H]thymidine and Na
125I incorporation into thyroid cell cultures have been examined. We found that serum and EGF could stimulate radioactively labelled thymidine incorporation into confluent cultures. However, both factors completely inhibited iodine uptake and organification at low concentrations. Insulin also stimulated [
Me-
3H]thymidine incorporation but had no adverse effect on thyroid differentiated function. TSH examined under the same conditions was not a growth factor but was essential to maintain differentiated functions. We conclude that thyroid growth and differentiation are not mutually exclusive processes. However, EGF and serum inhibit thyroid differentiated function at very low concentrations. Elucidation of the physiological role of these factors and their mechanism of action may lead to a greater understanding of thyroid hormone biosynthesis.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/0303-7207(84)90112-6</identifier><identifier>PMID: 6391978</identifier><identifier>CODEN: MCEND6</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Cell Differentiation ; Cells, Cultured ; DNA - biosynthesis ; epidermal growth factor ; Epidermal Growth Factor - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hormones. Regulation ; insulin ; Insulin - pharmacology ; Iodine Radioisotopes ; serum ; Sheep ; Thymidine - metabolism ; Thyroid Gland - cytology ; Thyroid Gland - metabolism ; Thyroid. Parathyroid. Ultimobranchial body ; thyrotropin ; Thyrotropin - pharmacology ; Vertebrates: endocrinology</subject><ispartof>Molecular and cellular endocrinology, 1984-12, Vol.38 (2), p.141-150</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-c9917246c85d2560066cd507ffb65fb9e8c3351194e0be8d4b8eadff0fe2681c3</citedby><cites>FETCH-LOGICAL-c386t-c9917246c85d2560066cd507ffb65fb9e8c3351194e0be8d4b8eadff0fe2681c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0303-7207(84)90112-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9235548$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6391978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eggo, M.C.</creatorcontrib><creatorcontrib>Bachrach, L.K.</creatorcontrib><creatorcontrib>Fayet, G.</creatorcontrib><creatorcontrib>Errick, J.</creatorcontrib><creatorcontrib>Kudlow, J.E.</creatorcontrib><creatorcontrib>Cohen, M.F.</creatorcontrib><creatorcontrib>Burrow, G.N.</creatorcontrib><title>The effects of growth factors and serum on DNA synthesis and differentiation in thyroid cells in culture</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>The effects of three putative growth factors and serum on [
Me-
3H]thymidine and Na
125I incorporation into thyroid cell cultures have been examined. We found that serum and EGF could stimulate radioactively labelled thymidine incorporation into confluent cultures. However, both factors completely inhibited iodine uptake and organification at low concentrations. Insulin also stimulated [
Me-
3H]thymidine incorporation but had no adverse effect on thyroid differentiated function. TSH examined under the same conditions was not a growth factor but was essential to maintain differentiated functions. We conclude that thyroid growth and differentiation are not mutually exclusive processes. However, EGF and serum inhibit thyroid differentiated function at very low concentrations. Elucidation of the physiological role of these factors and their mechanism of action may lead to a greater understanding of thyroid hormone biosynthesis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>DNA - biosynthesis</subject><subject>epidermal growth factor</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones. Regulation</subject><subject>insulin</subject><subject>Insulin - pharmacology</subject><subject>Iodine Radioisotopes</subject><subject>serum</subject><subject>Sheep</subject><subject>Thymidine - metabolism</subject><subject>Thyroid Gland - cytology</subject><subject>Thyroid Gland - metabolism</subject><subject>Thyroid. Parathyroid. Ultimobranchial body</subject><subject>thyrotropin</subject><subject>Thyrotropin - pharmacology</subject><subject>Vertebrates: endocrinology</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1q3DAUhUVpSadp3iAFLUpoF24l2frbBEL6C6HdJGshS1exgsdKJDll3r52Z5hlVwKd71wu30XonJJPlFDxmbSkbSQj8oPqPmpCKWvEC7ShSrJGES5fos0ReY3elPJACJGcqRN0IlpNtVQbNNwOgCEEcLXgFPB9Tn_qgIN1NeWC7eRxgTxvcZrwl19XuOymOkCJ-8jHpZlhqtHWuBBxwnXY5RQ9djCOZf1w81jnDG_Rq2DHAmeH9xTdfft6e_2jufn9_ef11U3jWiVq47SmknXCKe4ZF4QI4TwnMoRe8NBrUK5tOaW6A9KD8l2vwPoQSAAmFHXtKbrYz33M6WmGUs02lnUZO0Gai5FcUU0EW8BuD7qcSskQzGOOW5t3hhKzCjarPbPaM6oz_wQbsdTeHebP_Rb8sXQwuuTvD7ktzo4h28nFcsQ0aznvVuxyj8Hi4jlCNsVFmBz4mJdbGJ_i__f4CxrGl4o</recordid><startdate>198412</startdate><enddate>198412</enddate><creator>Eggo, M.C.</creator><creator>Bachrach, L.K.</creator><creator>Fayet, G.</creator><creator>Errick, J.</creator><creator>Kudlow, J.E.</creator><creator>Cohen, M.F.</creator><creator>Burrow, G.N.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198412</creationdate><title>The effects of growth factors and serum on DNA synthesis and differentiation in thyroid cells in culture</title><author>Eggo, M.C. ; Bachrach, L.K. ; Fayet, G. ; Errick, J. ; Kudlow, J.E. ; Cohen, M.F. ; Burrow, G.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-c9917246c85d2560066cd507ffb65fb9e8c3351194e0be8d4b8eadff0fe2681c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>DNA - biosynthesis</topic><topic>epidermal growth factor</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones. Regulation</topic><topic>insulin</topic><topic>Insulin - pharmacology</topic><topic>Iodine Radioisotopes</topic><topic>serum</topic><topic>Sheep</topic><topic>Thymidine - metabolism</topic><topic>Thyroid Gland - cytology</topic><topic>Thyroid Gland - metabolism</topic><topic>Thyroid. Parathyroid. Ultimobranchial body</topic><topic>thyrotropin</topic><topic>Thyrotropin - pharmacology</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eggo, M.C.</creatorcontrib><creatorcontrib>Bachrach, L.K.</creatorcontrib><creatorcontrib>Fayet, G.</creatorcontrib><creatorcontrib>Errick, J.</creatorcontrib><creatorcontrib>Kudlow, J.E.</creatorcontrib><creatorcontrib>Cohen, M.F.</creatorcontrib><creatorcontrib>Burrow, G.N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eggo, M.C.</au><au>Bachrach, L.K.</au><au>Fayet, G.</au><au>Errick, J.</au><au>Kudlow, J.E.</au><au>Cohen, M.F.</au><au>Burrow, G.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of growth factors and serum on DNA synthesis and differentiation in thyroid cells in culture</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1984-12</date><risdate>1984</risdate><volume>38</volume><issue>2</issue><spage>141</spage><epage>150</epage><pages>141-150</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><coden>MCEND6</coden><abstract>The effects of three putative growth factors and serum on [
Me-
3H]thymidine and Na
125I incorporation into thyroid cell cultures have been examined. We found that serum and EGF could stimulate radioactively labelled thymidine incorporation into confluent cultures. However, both factors completely inhibited iodine uptake and organification at low concentrations. Insulin also stimulated [
Me-
3H]thymidine incorporation but had no adverse effect on thyroid differentiated function. TSH examined under the same conditions was not a growth factor but was essential to maintain differentiated functions. We conclude that thyroid growth and differentiation are not mutually exclusive processes. However, EGF and serum inhibit thyroid differentiated function at very low concentrations. Elucidation of the physiological role of these factors and their mechanism of action may lead to a greater understanding of thyroid hormone biosynthesis.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>6391978</pmid><doi>10.1016/0303-7207(84)90112-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0303-7207 |
| ispartof | Molecular and cellular endocrinology, 1984-12, Vol.38 (2), p.141-150 |
| issn | 0303-7207 1872-8057 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75819062 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Cell Differentiation Cells, Cultured DNA - biosynthesis epidermal growth factor Epidermal Growth Factor - pharmacology Fundamental and applied biological sciences. Psychology Hormones. Regulation insulin Insulin - pharmacology Iodine Radioisotopes serum Sheep Thymidine - metabolism Thyroid Gland - cytology Thyroid Gland - metabolism Thyroid. Parathyroid. Ultimobranchial body thyrotropin Thyrotropin - pharmacology Vertebrates: endocrinology |
| title | The effects of growth factors and serum on DNA synthesis and differentiation in thyroid cells in culture |
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