Cationic gold staining of glomerular anionic sites in archived tissue, reprocessed from paraffin wax into LR gold resin
Glomerular capillary wall anionic sites have been demonstrated by cationic gold staining of archived renal biopsy tissue (up to 10 years old), obtained from six patients, originally embedded in paraffin wax, and subsequently reprocessed into LR gold resin. The staining patterns at pH 2.5 and pH 7.0,...
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| Veröffentlicht in: | The Histochemical journal 1993-05, Vol.25 (5), p.401-405 |
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| creator | GOODE, N. P SHIRES, M APARICIO, S. R DAVISON, A. M |
| description | Glomerular capillary wall anionic sites have been demonstrated by cationic gold staining of archived renal biopsy tissue (up to 10 years old), obtained from six patients, originally embedded in paraffin wax, and subsequently reprocessed into LR gold resin. The staining patterns at pH 2.5 and pH 7.0, demonstrating different glomerular basement membrane (GBM) anionic constituents, were compared in three patients from whom tissue directly processed into LR gold and reprocessed tissue was available. Ultrastructural preservation was poorer and shrinkage artefact greater in paraformaldehyde-lysine periodate (PLP) as opposed to formol saline-fixed reprocessed tissue. However, GBM anionic site expression was well preserved, or even enhanced (lamina rara externa, pH 7.0) in reprocessed tissue, using either fixative. Although it may not be possible to compare subtle changes in anionic site distribution in variously fixed and processed tissues, due to these artefacts, the technique enables retrospective study of charge status in archived material from disease groups in which there are distinct anionic site aberrations. |
| doi_str_mv | 10.1007/BF00159505 |
| format | Article |
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P ; SHIRES, M ; APARICIO, S. R ; DAVISON, A. M</creator><creatorcontrib>GOODE, N. P ; SHIRES, M ; APARICIO, S. R ; DAVISON, A. M</creatorcontrib><description>Glomerular capillary wall anionic sites have been demonstrated by cationic gold staining of archived renal biopsy tissue (up to 10 years old), obtained from six patients, originally embedded in paraffin wax, and subsequently reprocessed into LR gold resin. The staining patterns at pH 2.5 and pH 7.0, demonstrating different glomerular basement membrane (GBM) anionic constituents, were compared in three patients from whom tissue directly processed into LR gold and reprocessed tissue was available. Ultrastructural preservation was poorer and shrinkage artefact greater in paraformaldehyde-lysine periodate (PLP) as opposed to formol saline-fixed reprocessed tissue. However, GBM anionic site expression was well preserved, or even enhanced (lamina rara externa, pH 7.0) in reprocessed tissue, using either fixative. 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Psychology ; Gold ; Histocytological Preparation Techniques ; Humans ; Hydrogen-Ion Concentration ; Kidney Glomerulus - blood supply ; Kidney Glomerulus - chemistry ; Kidney Glomerulus - ultrastructure ; Male ; Microscopy, Electron ; Middle Aged ; Paraffin Embedding ; Staining and Labeling ; Vertebrates: urinary system</subject><ispartof>The Histochemical journal, 1993-05, Vol.25 (5), p.401-405</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c270t-7ed71433f10562d75616f21607409ef88ff52f82a5a281bfa1b491aa17a2ad613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4859658$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7686544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GOODE, N. P</creatorcontrib><creatorcontrib>SHIRES, M</creatorcontrib><creatorcontrib>APARICIO, S. R</creatorcontrib><creatorcontrib>DAVISON, A. M</creatorcontrib><title>Cationic gold staining of glomerular anionic sites in archived tissue, reprocessed from paraffin wax into LR gold resin</title><title>The Histochemical journal</title><addtitle>Histochem J</addtitle><description>Glomerular capillary wall anionic sites have been demonstrated by cationic gold staining of archived renal biopsy tissue (up to 10 years old), obtained from six patients, originally embedded in paraffin wax, and subsequently reprocessed into LR gold resin. The staining patterns at pH 2.5 and pH 7.0, demonstrating different glomerular basement membrane (GBM) anionic constituents, were compared in three patients from whom tissue directly processed into LR gold and reprocessed tissue was available. Ultrastructural preservation was poorer and shrinkage artefact greater in paraformaldehyde-lysine periodate (PLP) as opposed to formol saline-fixed reprocessed tissue. However, GBM anionic site expression was well preserved, or even enhanced (lamina rara externa, pH 7.0) in reprocessed tissue, using either fixative. Although it may not be possible to compare subtle changes in anionic site distribution in variously fixed and processed tissues, due to these artefacts, the technique enables retrospective study of charge status in archived material from disease groups in which there are distinct anionic site aberrations.</description><subject>Acrylic Resins</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Anions - analysis</subject><subject>Basement Membrane - chemistry</subject><subject>Basement Membrane - ultrastructure</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Capillaries - chemistry</subject><subject>Capillaries - ultrastructure</subject><subject>Child</subject><subject>Female</subject><subject>Fixatives</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold</subject><subject>Histocytological Preparation Techniques</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kidney Glomerulus - blood supply</subject><subject>Kidney Glomerulus - chemistry</subject><subject>Kidney Glomerulus - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Middle Aged</subject><subject>Paraffin Embedding</subject><subject>Staining and Labeling</subject><subject>Vertebrates: urinary system</subject><issn>0018-2214</issn><issn>1573-6865</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1Lw0AQxRdRav24eBf2IB7E6O4m-5GjFqtCQRA9h2myW1eSbN1JrP73prTU08B7v3kzPELOOLvhjOnb-yljXOaSyT0y5lKniTJK7pPxIJtECJ4dkiPET8ZYrrUakZFeA1k2JqsJdD60vqSLUFcUO_Ctbxc0OLqoQ2NjX0Ok0G4Y9J1F6lsKsfzw37ainUfs7TWNdhlDaREHzcXQ0CVEcG5AV_AzbHSBzl43N6JF356QAwc12tPtPCbv04e3yVMye3l8ntzNklJo1iXaVppnaeo4k0pUWiqunOCK6Yzl1hnjnBTOCJAgDJ874PMs5wBcg4BK8fSYXG5yh_e-eotd0XgsbV1Da0OPhZaGS8ayAbzagGUMiNG6Yhl9A_G34KxYt1z8tzzA59vUft7Yaoduax38i60PWELtIrSlxx2WGZkradI_kY-EWg</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>GOODE, N. P</creator><creator>SHIRES, M</creator><creator>APARICIO, S. R</creator><creator>DAVISON, A. M</creator><general>Kluwer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930501</creationdate><title>Cationic gold staining of glomerular anionic sites in archived tissue, reprocessed from paraffin wax into LR gold resin</title><author>GOODE, N. P ; SHIRES, M ; APARICIO, S. R ; DAVISON, A. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-7ed71433f10562d75616f21607409ef88ff52f82a5a281bfa1b491aa17a2ad613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acrylic Resins</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Anions - analysis</topic><topic>Basement Membrane - chemistry</topic><topic>Basement Membrane - ultrastructure</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Capillaries - chemistry</topic><topic>Capillaries - ultrastructure</topic><topic>Child</topic><topic>Female</topic><topic>Fixatives</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold</topic><topic>Histocytological Preparation Techniques</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kidney Glomerulus - blood supply</topic><topic>Kidney Glomerulus - chemistry</topic><topic>Kidney Glomerulus - ultrastructure</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Middle Aged</topic><topic>Paraffin Embedding</topic><topic>Staining and Labeling</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GOODE, N. P</creatorcontrib><creatorcontrib>SHIRES, M</creatorcontrib><creatorcontrib>APARICIO, S. R</creatorcontrib><creatorcontrib>DAVISON, A. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Histochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GOODE, N. P</au><au>SHIRES, M</au><au>APARICIO, S. R</au><au>DAVISON, A. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cationic gold staining of glomerular anionic sites in archived tissue, reprocessed from paraffin wax into LR gold resin</atitle><jtitle>The Histochemical journal</jtitle><addtitle>Histochem J</addtitle><date>1993-05-01</date><risdate>1993</risdate><volume>25</volume><issue>5</issue><spage>401</spage><epage>405</epage><pages>401-405</pages><issn>0018-2214</issn><eissn>1573-6865</eissn><coden>HISJAE</coden><abstract>Glomerular capillary wall anionic sites have been demonstrated by cationic gold staining of archived renal biopsy tissue (up to 10 years old), obtained from six patients, originally embedded in paraffin wax, and subsequently reprocessed into LR gold resin. The staining patterns at pH 2.5 and pH 7.0, demonstrating different glomerular basement membrane (GBM) anionic constituents, were compared in three patients from whom tissue directly processed into LR gold and reprocessed tissue was available. Ultrastructural preservation was poorer and shrinkage artefact greater in paraformaldehyde-lysine periodate (PLP) as opposed to formol saline-fixed reprocessed tissue. However, GBM anionic site expression was well preserved, or even enhanced (lamina rara externa, pH 7.0) in reprocessed tissue, using either fixative. Although it may not be possible to compare subtle changes in anionic site distribution in variously fixed and processed tissues, due to these artefacts, the technique enables retrospective study of charge status in archived material from disease groups in which there are distinct anionic site aberrations.</abstract><cop>London</cop><pub>Kluwer</pub><pmid>7686544</pmid><doi>10.1007/BF00159505</doi><tpages>5</tpages></addata></record> |
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| subjects | Acrylic Resins Adolescent Adult Anions - analysis Basement Membrane - chemistry Basement Membrane - ultrastructure Binding Sites Biological and medical sciences Biopsy Capillaries - chemistry Capillaries - ultrastructure Child Female Fixatives Fundamental and applied biological sciences. Psychology Gold Histocytological Preparation Techniques Humans Hydrogen-Ion Concentration Kidney Glomerulus - blood supply Kidney Glomerulus - chemistry Kidney Glomerulus - ultrastructure Male Microscopy, Electron Middle Aged Paraffin Embedding Staining and Labeling Vertebrates: urinary system |
| title | Cationic gold staining of glomerular anionic sites in archived tissue, reprocessed from paraffin wax into LR gold resin |
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