Inward barium current and excitation-contraction coupling in frog twitch muscle fibres
The role of barium ions in excitation-contraction coupling was studied in single isolated frog semitendinosus fibres. Simultaneous recordings of membrane currents and contraction under voltage-clamp conditions in a sucrose-vaseline gap device show that barium ions have a reversible inhibiting effect...
Gespeichert in:
| Veröffentlicht in: | Journal of muscle research and cell motility 1993-04, Vol.14 (2), p.158-166 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 166 |
|---|---|
| container_issue | 2 |
| container_start_page | 158 |
| container_title | Journal of muscle research and cell motility |
| container_volume | 14 |
| creator | BLAINEAU, S JACQUEMOND, V ALLARD, B AMSELLEM, J MOUTIN, M.-J ROUGIER, O |
| description | The role of barium ions in excitation-contraction coupling was studied in single isolated frog semitendinosus fibres. Simultaneous recordings of membrane currents and contraction under voltage-clamp conditions in a sucrose-vaseline gap device show that barium ions have a reversible inhibiting effect on contraction. This inhibiting action was correlated to the entry of barium ions via the DHP-sensitive tubular calcium channel. Cytological observations and X-ray microanalysis performed on the fibres used in the electrophysiological experiments indicate that barium ions do not accumulate in the junctional sarcoplasmic reticulum; they can freely diffuse in the intermyofibrillar space and they accumulate in mitochondria. Calcium release experiments performed on isolated sarcoplasmic reticulum vesicles show that barium ions are not able to induce calcium release from calcium-loaded vesicles, they behave as calcium release inhibitors. These results are discussed in relation with the possible role of the slow Ca current in excitation-contraction coupling. |
| doi_str_mv | 10.1007/bf00115450 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75814609</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16633504</sourcerecordid><originalsourceid>FETCH-LOGICAL-c371t-bdd5aa3e621ee21c7f70b5792eff6b4fff5a835547f567418635a3d0e91a46a3</originalsourceid><addsrcrecordid>eNqFkUtLxDAUhYMo4_jYuBeyEBdCNbd5tUsVHwMDbga35TZNNNKmY9Iy-u8dsbp1dQ-cj7P4LiEnwC6BMX1VO8YApJBsh8xBap7lSupdMmcg8kxwKPfJQUpvjDFZ5vmMzAoOkkE5J8-LsMHY0BqjHztqxhhtGCiGhtoP4wccfB8y04chovnO1PTjuvXhhfpAXexf6LDxg3ml3ZhMa6nzdbTpiOw5bJM9nu4hWd3frW4fs-XTw-L2epkZrmHI6qaRiNyqHKzNwWinWS11mVvnVC2ccxILLqXQTiotoFBcIm-YLQGFQn5Izn9m17F_H20aqs4nY9sWg-3HVGlZgFCs_BcEpTiXTGzBix_QxD6laF21jr7D-FkBq75lVzf3v7K38Om0Otadbf7Qye62P5t6TAZbFzEYn_4wXvLtnxT_AsMVhr0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16633504</pqid></control><display><type>article</type><title>Inward barium current and excitation-contraction coupling in frog twitch muscle fibres</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>BLAINEAU, S ; JACQUEMOND, V ; ALLARD, B ; AMSELLEM, J ; MOUTIN, M.-J ; ROUGIER, O</creator><creatorcontrib>BLAINEAU, S ; JACQUEMOND, V ; ALLARD, B ; AMSELLEM, J ; MOUTIN, M.-J ; ROUGIER, O</creatorcontrib><description>The role of barium ions in excitation-contraction coupling was studied in single isolated frog semitendinosus fibres. Simultaneous recordings of membrane currents and contraction under voltage-clamp conditions in a sucrose-vaseline gap device show that barium ions have a reversible inhibiting effect on contraction. This inhibiting action was correlated to the entry of barium ions via the DHP-sensitive tubular calcium channel. Cytological observations and X-ray microanalysis performed on the fibres used in the electrophysiological experiments indicate that barium ions do not accumulate in the junctional sarcoplasmic reticulum; they can freely diffuse in the intermyofibrillar space and they accumulate in mitochondria. Calcium release experiments performed on isolated sarcoplasmic reticulum vesicles show that barium ions are not able to induce calcium release from calcium-loaded vesicles, they behave as calcium release inhibitors. These results are discussed in relation with the possible role of the slow Ca current in excitation-contraction coupling.</description><identifier>ISSN: 0142-4319</identifier><identifier>EISSN: 1573-2657</identifier><identifier>DOI: 10.1007/bf00115450</identifier><identifier>PMID: 8315019</identifier><identifier>CODEN: JMRMD3</identifier><language>eng</language><publisher>London: Kluwer Academic Publishers</publisher><subject>Animals ; Anura ; Barium - metabolism ; Biological and medical sciences ; Calcium - analysis ; Calcium - metabolism ; Cell physiology ; Electric Stimulation ; Electron Probe Microanalysis ; Electrophysiology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Membrane Potentials ; Mitochondria, Muscle - metabolism ; Molecular and cellular biology ; Muscle Contraction ; Muscles - physiology ; Muscles - ultrastructure ; Rabbits ; Ranidae ; Sarcoplasmic Reticulum - metabolism</subject><ispartof>Journal of muscle research and cell motility, 1993-04, Vol.14 (2), p.158-166</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-bdd5aa3e621ee21c7f70b5792eff6b4fff5a835547f567418635a3d0e91a46a3</citedby><cites>FETCH-LOGICAL-c371t-bdd5aa3e621ee21c7f70b5792eff6b4fff5a835547f567418635a3d0e91a46a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>310,311,315,781,785,790,791,23935,23936,25145,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3932656$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8315019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BLAINEAU, S</creatorcontrib><creatorcontrib>JACQUEMOND, V</creatorcontrib><creatorcontrib>ALLARD, B</creatorcontrib><creatorcontrib>AMSELLEM, J</creatorcontrib><creatorcontrib>MOUTIN, M.-J</creatorcontrib><creatorcontrib>ROUGIER, O</creatorcontrib><title>Inward barium current and excitation-contraction coupling in frog twitch muscle fibres</title><title>Journal of muscle research and cell motility</title><addtitle>J Muscle Res Cell Motil</addtitle><description>The role of barium ions in excitation-contraction coupling was studied in single isolated frog semitendinosus fibres. Simultaneous recordings of membrane currents and contraction under voltage-clamp conditions in a sucrose-vaseline gap device show that barium ions have a reversible inhibiting effect on contraction. This inhibiting action was correlated to the entry of barium ions via the DHP-sensitive tubular calcium channel. Cytological observations and X-ray microanalysis performed on the fibres used in the electrophysiological experiments indicate that barium ions do not accumulate in the junctional sarcoplasmic reticulum; they can freely diffuse in the intermyofibrillar space and they accumulate in mitochondria. Calcium release experiments performed on isolated sarcoplasmic reticulum vesicles show that barium ions are not able to induce calcium release from calcium-loaded vesicles, they behave as calcium release inhibitors. These results are discussed in relation with the possible role of the slow Ca current in excitation-contraction coupling.</description><subject>Animals</subject><subject>Anura</subject><subject>Barium - metabolism</subject><subject>Biological and medical sciences</subject><subject>Calcium - analysis</subject><subject>Calcium - metabolism</subject><subject>Cell physiology</subject><subject>Electric Stimulation</subject><subject>Electron Probe Microanalysis</subject><subject>Electrophysiology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Membrane Potentials</subject><subject>Mitochondria, Muscle - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Muscle Contraction</subject><subject>Muscles - physiology</subject><subject>Muscles - ultrastructure</subject><subject>Rabbits</subject><subject>Ranidae</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><issn>0142-4319</issn><issn>1573-2657</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLxDAUhYMo4_jYuBeyEBdCNbd5tUsVHwMDbga35TZNNNKmY9Iy-u8dsbp1dQ-cj7P4LiEnwC6BMX1VO8YApJBsh8xBap7lSupdMmcg8kxwKPfJQUpvjDFZ5vmMzAoOkkE5J8-LsMHY0BqjHztqxhhtGCiGhtoP4wccfB8y04chovnO1PTjuvXhhfpAXexf6LDxg3ml3ZhMa6nzdbTpiOw5bJM9nu4hWd3frW4fs-XTw-L2epkZrmHI6qaRiNyqHKzNwWinWS11mVvnVC2ccxILLqXQTiotoFBcIm-YLQGFQn5Izn9m17F_H20aqs4nY9sWg-3HVGlZgFCs_BcEpTiXTGzBix_QxD6laF21jr7D-FkBq75lVzf3v7K38Om0Otadbf7Qye62P5t6TAZbFzEYn_4wXvLtnxT_AsMVhr0</recordid><startdate>19930401</startdate><enddate>19930401</enddate><creator>BLAINEAU, S</creator><creator>JACQUEMOND, V</creator><creator>ALLARD, B</creator><creator>AMSELLEM, J</creator><creator>MOUTIN, M.-J</creator><creator>ROUGIER, O</creator><general>Kluwer Academic Publishers</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19930401</creationdate><title>Inward barium current and excitation-contraction coupling in frog twitch muscle fibres</title><author>BLAINEAU, S ; JACQUEMOND, V ; ALLARD, B ; AMSELLEM, J ; MOUTIN, M.-J ; ROUGIER, O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-bdd5aa3e621ee21c7f70b5792eff6b4fff5a835547f567418635a3d0e91a46a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Anura</topic><topic>Barium - metabolism</topic><topic>Biological and medical sciences</topic><topic>Calcium - analysis</topic><topic>Calcium - metabolism</topic><topic>Cell physiology</topic><topic>Electric Stimulation</topic><topic>Electron Probe Microanalysis</topic><topic>Electrophysiology</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Membrane Potentials</topic><topic>Mitochondria, Muscle - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Muscle Contraction</topic><topic>Muscles - physiology</topic><topic>Muscles - ultrastructure</topic><topic>Rabbits</topic><topic>Ranidae</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BLAINEAU, S</creatorcontrib><creatorcontrib>JACQUEMOND, V</creatorcontrib><creatorcontrib>ALLARD, B</creatorcontrib><creatorcontrib>AMSELLEM, J</creatorcontrib><creatorcontrib>MOUTIN, M.-J</creatorcontrib><creatorcontrib>ROUGIER, O</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of muscle research and cell motility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BLAINEAU, S</au><au>JACQUEMOND, V</au><au>ALLARD, B</au><au>AMSELLEM, J</au><au>MOUTIN, M.-J</au><au>ROUGIER, O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inward barium current and excitation-contraction coupling in frog twitch muscle fibres</atitle><jtitle>Journal of muscle research and cell motility</jtitle><addtitle>J Muscle Res Cell Motil</addtitle><date>1993-04-01</date><risdate>1993</risdate><volume>14</volume><issue>2</issue><spage>158</spage><epage>166</epage><pages>158-166</pages><issn>0142-4319</issn><eissn>1573-2657</eissn><coden>JMRMD3</coden><abstract>The role of barium ions in excitation-contraction coupling was studied in single isolated frog semitendinosus fibres. Simultaneous recordings of membrane currents and contraction under voltage-clamp conditions in a sucrose-vaseline gap device show that barium ions have a reversible inhibiting effect on contraction. This inhibiting action was correlated to the entry of barium ions via the DHP-sensitive tubular calcium channel. Cytological observations and X-ray microanalysis performed on the fibres used in the electrophysiological experiments indicate that barium ions do not accumulate in the junctional sarcoplasmic reticulum; they can freely diffuse in the intermyofibrillar space and they accumulate in mitochondria. Calcium release experiments performed on isolated sarcoplasmic reticulum vesicles show that barium ions are not able to induce calcium release from calcium-loaded vesicles, they behave as calcium release inhibitors. These results are discussed in relation with the possible role of the slow Ca current in excitation-contraction coupling.</abstract><cop>London</cop><cop>Boston</cop><cop>Dordrecht</cop><pub>Kluwer Academic Publishers</pub><pmid>8315019</pmid><doi>10.1007/bf00115450</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-4319 |
| ispartof | Journal of muscle research and cell motility, 1993-04, Vol.14 (2), p.158-166 |
| issn | 0142-4319 1573-2657 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75814609 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Anura Barium - metabolism Biological and medical sciences Calcium - analysis Calcium - metabolism Cell physiology Electric Stimulation Electron Probe Microanalysis Electrophysiology Freshwater Fundamental and applied biological sciences. Psychology Membrane Potentials Mitochondria, Muscle - metabolism Molecular and cellular biology Muscle Contraction Muscles - physiology Muscles - ultrastructure Rabbits Ranidae Sarcoplasmic Reticulum - metabolism |
| title | Inward barium current and excitation-contraction coupling in frog twitch muscle fibres |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-11T21%3A49%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inward%20barium%20current%20and%20excitation-contraction%20coupling%20in%20frog%20twitch%20muscle%20fibres&rft.jtitle=Journal%20of%20muscle%20research%20and%20cell%20motility&rft.au=BLAINEAU,%20S&rft.date=1993-04-01&rft.volume=14&rft.issue=2&rft.spage=158&rft.epage=166&rft.pages=158-166&rft.issn=0142-4319&rft.eissn=1573-2657&rft.coden=JMRMD3&rft_id=info:doi/10.1007/bf00115450&rft_dat=%3Cproquest_cross%3E16633504%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16633504&rft_id=info:pmid/8315019&rfr_iscdi=true |