Multiplex PCR detection of slowly-evolving trypanosomatids and neogregarines in bumblebees using broad-range primers

The aims of this study were to design universal markers for different protozoan parasites of Bombus spp. based on the phylogenetic position of two important bumblebee parasites Crithidia bombi and Apicystis bombi. Standard PCR and extraction techniques were used to amplify and sequence 18S rDNA. Phy...

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Veröffentlicht in:	Journal of applied microbiology 2010-07, Vol.109 (1), p.107-115
Hauptverfasser:	Meeus, I, de Graaf, D.C, Jans, K, Smagghe, G
Format:	Artikel
Sprache:	eng
Schlagworte:	18S rDNA Animals Apicomplexa - genetics Apicomplexa - isolation & purification Apicystis bombi Bees - parasitology Biological and medical sciences Bombus Bumblebees Crithidia - genetics Crithidia - isolation & purification Crithidia bombi DNA Primers - genetics DNA, Protozoan - genetics Evolution Fundamental and applied biological sciences. Psychology Insects Markers Microbiology Multiplexing Parasites Phylogenetics Phylogeny Polymerase Chain Reaction - methods Protozoa protozoa detection RNA, Ribosomal, 18S - genetics rRNA Sequence Analysis, DNA Systematics
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