Overexpression of Bax inhibitor-1 (BI-1) induces cell transformation in NIH3T3 cells
BI‐1 (Bax inhibitor‐1), an apoptosis‐inhibiting gene belonging to the Bcl‐2 protein family, plays an important role in mitochondrial apoptosis pathway to suppress Bax‐induced apoptosis. To investigate the potential role of BI‐1 in promoting cell growth and tumorigenesis, in the present study we over...
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| Veröffentlicht in: | Cell biology international 2010-11, Vol.34 (11), p.1099-1104 |
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| container_title | Cell biology international |
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| creator | Xiang-yong, Li Yang-chao, Chen Ke-yuan, Zhou Mei-hong, Zhang Hai-qing, Luo Hsiang-fu, Kung Xin, Zhou |
| description | BI‐1 (Bax inhibitor‐1), an apoptosis‐inhibiting gene belonging to the Bcl‐2 protein family, plays an important role in mitochondrial apoptosis pathway to suppress Bax‐induced apoptosis. To investigate the potential role of BI‐1 in promoting cell growth and tumorigenesis, in the present study we overexpressed the BI‐1 gene in NIH3T3 cells using the lentivirus‐mediated gene expression system. Our in vitro studies showed that NIH3T3 cells overexpressing BI‐1 displayed a significantly higher growth rate and formed more and larger colonies than the control cells. In addition, our in vivo studies indicated that the lenti‐BI‐1‐infected cells formed obvious tumours, while no tumours were formed by the control cells after subcutaneously injected into nude mice. These results strongly suggested that the BI‐1 gene might play a crucial role in neoplastic genesis and development. |
| doi_str_mv | 10.1042/CBI20090400 |
| format | Article |
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To investigate the potential role of BI‐1 in promoting cell growth and tumorigenesis, in the present study we overexpressed the BI‐1 gene in NIH3T3 cells using the lentivirus‐mediated gene expression system. Our in vitro studies showed that NIH3T3 cells overexpressing BI‐1 displayed a significantly higher growth rate and formed more and larger colonies than the control cells. In addition, our in vivo studies indicated that the lenti‐BI‐1‐infected cells formed obvious tumours, while no tumours were formed by the control cells after subcutaneously injected into nude mice. These results strongly suggested that the BI‐1 gene might play a crucial role in neoplastic genesis and development.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1042/CBI20090400</identifier><identifier>PMID: 20597862</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Apoptosis ; Apoptosis Regulatory Proteins - genetics ; Apoptosis Regulatory Proteins - metabolism ; BI-1 ; cell growth ; Cell Transformation, Neoplastic - genetics ; Cells, Cultured ; Gene Expression Regulation ; Humans ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Mice ; Mice, Nude ; NIH 3T3 Cells ; Transfection ; transformation ; tumorigenicity</subject><ispartof>Cell biology international, 2010-11, Vol.34 (11), p.1099-1104</ispartof><rights>2010 International Federation for Cell Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3683-cace6dee2f0393afcdc86224f0786356bc3eae6e7d591c9cfc4a3558b051d0723</citedby><cites>FETCH-LOGICAL-c3683-cace6dee2f0393afcdc86224f0786356bc3eae6e7d591c9cfc4a3558b051d0723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1042%2FCBI20090400$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1042%2FCBI20090400$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20597862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiang-yong, Li</creatorcontrib><creatorcontrib>Yang-chao, Chen</creatorcontrib><creatorcontrib>Ke-yuan, Zhou</creatorcontrib><creatorcontrib>Mei-hong, Zhang</creatorcontrib><creatorcontrib>Hai-qing, Luo</creatorcontrib><creatorcontrib>Hsiang-fu, Kung</creatorcontrib><creatorcontrib>Xin, Zhou</creatorcontrib><title>Overexpression of Bax inhibitor-1 (BI-1) induces cell transformation in NIH3T3 cells</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>BI‐1 (Bax inhibitor‐1), an apoptosis‐inhibiting gene belonging to the Bcl‐2 protein family, plays an important role in mitochondrial apoptosis pathway to suppress Bax‐induced apoptosis. To investigate the potential role of BI‐1 in promoting cell growth and tumorigenesis, in the present study we overexpressed the BI‐1 gene in NIH3T3 cells using the lentivirus‐mediated gene expression system. Our in vitro studies showed that NIH3T3 cells overexpressing BI‐1 displayed a significantly higher growth rate and formed more and larger colonies than the control cells. In addition, our in vivo studies indicated that the lenti‐BI‐1‐infected cells formed obvious tumours, while no tumours were formed by the control cells after subcutaneously injected into nude mice. These results strongly suggested that the BI‐1 gene might play a crucial role in neoplastic genesis and development.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis Regulatory Proteins - genetics</subject><subject>Apoptosis Regulatory Proteins - metabolism</subject><subject>BI-1</subject><subject>cell growth</subject><subject>Cell Transformation, Neoplastic - genetics</subject><subject>Cells, Cultured</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>NIH 3T3 Cells</subject><subject>Transfection</subject><subject>transformation</subject><subject>tumorigenicity</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFPwjAUxhujEUVP3s1uasy07Vu77ShEgUjgIMZjU7q3WIUN26Hw37sJEk-e3svr7_vy9SPkjNEbRiN-2-0MOKUpjSjdI0eMpiJMQIj9ZpcilGkqWuTY-zdKGYsSeUhanIo0TiQ_IpPxJzpcLRx6b8siKPOgo1eBLV7t1FalC1lw2RmE7Ko-ZUuDPjA4mwWV04XPSzfXVaOyRTAa9GECP6_-hBzkeubxdDvb5PnhftLth8Nxb9C9G4YGZAKh0QZlhshzCino3GSmzsSjnNbZQMipAdQoMc5EykxqchPp-mPJlAqW0ZhDm1xsfBeu_Fiir9Tc-iaBLrBcehWLhIFgwGryekMaV3rvMFcLZ-farRWjqmlR_Wmxps-3vsvpHLMd-1tbDbAN8GVnuP7Pq9lHwAFqTbjRWF_haqfR7l3JGGKhXkY9xUeTDjzGT6oP33UTiJA</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Xiang-yong, Li</creator><creator>Yang-chao, Chen</creator><creator>Ke-yuan, Zhou</creator><creator>Mei-hong, Zhang</creator><creator>Hai-qing, Luo</creator><creator>Hsiang-fu, Kung</creator><creator>Xin, Zhou</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201011</creationdate><title>Overexpression of Bax inhibitor-1 (BI-1) induces cell transformation in NIH3T3 cells</title><author>Xiang-yong, Li ; Yang-chao, Chen ; Ke-yuan, Zhou ; Mei-hong, Zhang ; Hai-qing, Luo ; Hsiang-fu, Kung ; Xin, Zhou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3683-cace6dee2f0393afcdc86224f0786356bc3eae6e7d591c9cfc4a3558b051d0723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis Regulatory Proteins - genetics</topic><topic>Apoptosis Regulatory Proteins - metabolism</topic><topic>BI-1</topic><topic>cell growth</topic><topic>Cell Transformation, Neoplastic - genetics</topic><topic>Cells, Cultured</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>NIH 3T3 Cells</topic><topic>Transfection</topic><topic>transformation</topic><topic>tumorigenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiang-yong, Li</creatorcontrib><creatorcontrib>Yang-chao, Chen</creatorcontrib><creatorcontrib>Ke-yuan, Zhou</creatorcontrib><creatorcontrib>Mei-hong, Zhang</creatorcontrib><creatorcontrib>Hai-qing, Luo</creatorcontrib><creatorcontrib>Hsiang-fu, Kung</creatorcontrib><creatorcontrib>Xin, Zhou</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiang-yong, Li</au><au>Yang-chao, Chen</au><au>Ke-yuan, Zhou</au><au>Mei-hong, Zhang</au><au>Hai-qing, Luo</au><au>Hsiang-fu, Kung</au><au>Xin, Zhou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Bax inhibitor-1 (BI-1) induces cell transformation in NIH3T3 cells</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2010-11</date><risdate>2010</risdate><volume>34</volume><issue>11</issue><spage>1099</spage><epage>1104</epage><pages>1099-1104</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>BI‐1 (Bax inhibitor‐1), an apoptosis‐inhibiting gene belonging to the Bcl‐2 protein family, plays an important role in mitochondrial apoptosis pathway to suppress Bax‐induced apoptosis. To investigate the potential role of BI‐1 in promoting cell growth and tumorigenesis, in the present study we overexpressed the BI‐1 gene in NIH3T3 cells using the lentivirus‐mediated gene expression system. Our in vitro studies showed that NIH3T3 cells overexpressing BI‐1 displayed a significantly higher growth rate and formed more and larger colonies than the control cells. In addition, our in vivo studies indicated that the lenti‐BI‐1‐infected cells formed obvious tumours, while no tumours were formed by the control cells after subcutaneously injected into nude mice. These results strongly suggested that the BI‐1 gene might play a crucial role in neoplastic genesis and development.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20597862</pmid><doi>10.1042/CBI20090400</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Apoptosis Apoptosis Regulatory Proteins - genetics Apoptosis Regulatory Proteins - metabolism BI-1 cell growth Cell Transformation, Neoplastic - genetics Cells, Cultured Gene Expression Regulation Humans Membrane Proteins - genetics Membrane Proteins - metabolism Mice Mice, Nude NIH 3T3 Cells Transfection transformation tumorigenicity |
| title | Overexpression of Bax inhibitor-1 (BI-1) induces cell transformation in NIH3T3 cells |
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