Electro-immortalization of B-lymphocytes isolated from stomach carcinoma biopsy material

The advantages of electrofusion were used to immortalize the small number of B-cells from fresh biopsy material taken from a gastric carcinoma of a patient. Two stable human antibody secreting clones could be produced which exhibited functional activity against the autologous tumour cells (inhibitio...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Hybridoma 1993-04, Vol.12 (2), p.221-225
Hauptverfasser: VOLLMERS, H. P, LANDENBERG, P. V, DÄMMRICH, J, STULLE, K, WOZNIAK, E, RINGDÖRFER, C, MÜLLER-HERMELINK, H. K, HERRMANN, B, ZIMMERMANN, U
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The advantages of electrofusion were used to immortalize the small number of B-cells from fresh biopsy material taken from a gastric carcinoma of a patient. Two stable human antibody secreting clones could be produced which exhibited functional activity against the autologous tumour cells (inhibition of cell adhesion and immunofluorescence staining of the membranes). This shows that a variety of hitherto inaccessible B lymphocyte populations from other human organ biopsies can be immortalised by the improved electrofusion technique.
ISSN:0272-457X
2168-7897
0272-457X
DOI:10.1089/hyb.1993.12.221