Fluorescent estimation on the effect of Ca2+ antagonists on the oxidative metabolism in dissociated mammalian brain neurons
Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidized to be fluorescent by cellular oxidants. Of the organic Ca2+ antago...
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| Veröffentlicht in: | Brain research 1993-04, Vol.610 (1), p.172-175 |
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| container_title | Brain research |
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| creator | Oyama, Y Hayashi, A Ueha, T Chikahisa, L Furukawa, K |
| description | Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidized to be fluorescent by cellular oxidants. Of the organic Ca2+ antagonists used, flunarizine and nifedipine decreased the intensity of fluorescence at 1 microM or more while it was not the case for verapamil and diltiazem, suggesting one of the favourable actions of flunarizine and nifedipine on ischemic brain damage. |
| doi_str_mv | 10.1016/0006-8993(93)91234-j |
| format | Article |
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| ispartof | Brain research, 1993-04, Vol.610 (1), p.172-175 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Brain - cytology Brain - drug effects Brain - metabolism Calcium Channel Blockers - pharmacology Flow Cytometry Fluoresceins In Vitro Techniques Neurons - drug effects Neurons - metabolism Oxidation-Reduction Rats Rats, Wistar Spectrometry, Fluorescence |
| title | Fluorescent estimation on the effect of Ca2+ antagonists on the oxidative metabolism in dissociated mammalian brain neurons |
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