Optimization of the synthesis of porcine somatotropin in Escherichia coli

We report on the influence of choice of promoter and RNA polymerase, 5'-untranslated regions and ribosome binding sites, codon usage, leader peptide coding sequences and poly A tail in the 3'-untranslated region on the synthesis of porcine somatotropin (PST) in Escherichia coli. A total of...

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Veröffentlicht in:	Applied microbiology and biotechnology 1993-06, Vol.39 (3), p.324-328
Hauptverfasser:	Wang, H, O'Mahony, D.J, McConnell, D.J, Qi, S.Z
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular Codon - genetics Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Genetic Vectors Growth Hormone - biosynthesis Growth Hormone - genetics Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data optimization Plasmids - genetics Protein Biosynthesis Protein Sorting Signals - genetics Recombinant Proteins - biosynthesis Recombinant Proteins - genetics somatotropin Swine synthesis
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