Detection of K-ras mutation in sputum by mutant-allele-specific amplification (MASA)
From 10 to 30% of lung carcinomas examined to date contain mutant K‐ras genes. We report here that the mutant‐allele‐specific amplification (MASA) method may be useful for detection of the K‐ras mutations in cells obtained from the sputum of patients with lung cancer. The PCR product from one of fiv...
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| Veröffentlicht in: | Human mutation 1993, Vol.2 (2), p.112-117 |
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| container_title | Human mutation |
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| creator | Takeda, Satoshi Ichii, Shigetoshi Nakamura, Yusuke |
| description | From 10 to 30% of lung carcinomas examined to date contain mutant K‐ras genes. We report here that the mutant‐allele‐specific amplification (MASA) method may be useful for detection of the K‐ras mutations in cells obtained from the sputum of patients with lung cancer. The PCR product from one of five patients revealed an alteration when mixed oligonucleotides representing variants of the second letter at codon 12 of this gene were used as 5′ primers, and further experiments showed a mutation of GGT (Gly) to GAT (Asp)at codon 12. The MASA system could also be applied to an examination of metastatic lung carcinomas, particularly from adenocarcinomas in colon and pancreas in which frequent K‐ras mutations are detected, and to mass‐screening for colorectal tumors using DNA isolated from feces as template. © 1993 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/humu.1380020209 |
| format | Article |
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Mutat</addtitle><date>1993</date><risdate>1993</risdate><volume>2</volume><issue>2</issue><spage>112</spage><epage>117</epage><pages>112-117</pages><issn>1059-7794</issn><eissn>1098-1004</eissn><abstract>From 10 to 30% of lung carcinomas examined to date contain mutant K‐ras genes. We report here that the mutant‐allele‐specific amplification (MASA) method may be useful for detection of the K‐ras mutations in cells obtained from the sputum of patients with lung cancer. The PCR product from one of five patients revealed an alteration when mixed oligonucleotides representing variants of the second letter at codon 12 of this gene were used as 5′ primers, and further experiments showed a mutation of GGT (Gly) to GAT (Asp)at codon 12. 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| source | Wiley-Blackwell Journals; MEDLINE |
| subjects | Alleles Amino Acid Sequence Base Sequence DNA Mutational Analysis - methods DNA, Neoplasm - genetics Evaluation Studies as Topic Genes, ras Humans K-ras Lung Neoplasms - genetics MASA Molecular Sequence Data Nucleic Acid Amplification Techniques Sputum Sputum - chemistry |
| title | Detection of K-ras mutation in sputum by mutant-allele-specific amplification (MASA) |
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