Cloning, Chromosomal Mapping, and Expression of Human Fetal Brain Type I Adenylyl Cyclase
The neural-specific calmodulin-sensitive adenylyl cyclase (type I), which was first cloned from bovine brain, has been implicated in learning and memory. The objective of this study was to clone and determine the chromosomal localization of human fetal brain type I adenylyl cyclase. A 3.8-kb cDNA cl...
Gespeichert in:
| Veröffentlicht in: | Genomics 1993-05, Vol.16 (2), p.473-478 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 478 |
|---|---|
| container_issue | 2 |
| container_start_page | 473 |
| container_title | Genomics |
| container_volume | 16 |
| creator | Villacres, Enrique C. Xia, Zhengui Bookbinder, Louis H. Edelhoff, Susanne Disteche, Christine M. Storm, Daniel R. |
| description | The neural-specific calmodulin-sensitive adenylyl cyclase (type I), which was first cloned from bovine brain, has been implicated in learning and memory. The objective of this study was to clone and determine the chromosomal localization of human fetal brain type I adenylyl cyclase. A 3.8-kb cDNA clone was isolated that contained sequence coinciding with the 3′ end 2553 nucleotides of the bovine open reading frame. This clone shows 87% nucleotide and 92% translated amino acid sequence identity to the bovine clone. The most significant sequence differences were in the carboxy-terminal 100 amino acid residues. This region contains one of several possible calmodulin binding domains and the only putative cAMP-dependent protein kinase A phosphorylation site. A chimera was constructed that contained the 5′ half of the bovine type I adenylyl cyclase and the 3′ half of the human type I adenylyl cyclase. The activity of the chimeric gene product and its sensitivity to calmodulin and calcium were indistinguishable from those of the bovine type I adenylyl cyclase.
In situ hybridization was used to localize the human type I adenylyl cyclase gene to the proximal portion of the short arm of chromosome 7. |
| doi_str_mv | 10.1006/geno.1993.1213 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_proquest_miscellaneous_75802950</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0888754383712139</els_id><sourcerecordid>75802950</sourcerecordid><originalsourceid>FETCH-LOGICAL-c393t-2ffe8678d2f58be0cdd9b459afa6267ec5986e9f979770c9432319fa2b76cfe93</originalsourceid><addsrcrecordid>eNp1kE1v1DAQhi1EVbalV25IPiBOZPFHHNvHErW0Uisu5dCT5XXGrVFiBzuLyL8n6a564zTSzDOjeR-EPlCypYQ0X58gpi3Vmm8po_wN2lCidKWaunmLNkQpVUlR83forJRfhBDNFTtFp4rTWiixQY9tn2KIT19w-5zTkEoabI_v7Ti-NG3s8NXfMUMpIUWcPL7ZDzbia5gW7Fu2IeKHeQR8iy87iHM_97idXW8LvEcn3vYFLo71HP28vnpob6q7H99v28u7ynHNp4p5D6qRqmNeqB0Q13V6VwttvW1YI8EJrRrQXkstJXG65oxT7S3bycZ50Pwc4cPdVKZgigsTuGeXYgQ3Gaa0rBfk8wEZc_q9hzKZIRQHfW8jpH0xUijCtCALuD2ALqdSMngz5jDYPBtKzKrbrLrNqtusupeFj8fL-90A3St-9LvMPx3ntjjb-2yjC-UVq6VigrAFUwcMFlF_AuQ1B0QHXchrjC6F_33wD55Rmp0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75802950</pqid></control><display><type>article</type><title>Cloning, Chromosomal Mapping, and Expression of Human Fetal Brain Type I Adenylyl Cyclase</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Villacres, Enrique C. ; Xia, Zhengui ; Bookbinder, Louis H. ; Edelhoff, Susanne ; Disteche, Christine M. ; Storm, Daniel R.</creator><creatorcontrib>Villacres, Enrique C. ; Xia, Zhengui ; Bookbinder, Louis H. ; Edelhoff, Susanne ; Disteche, Christine M. ; Storm, Daniel R.</creatorcontrib><description>The neural-specific calmodulin-sensitive adenylyl cyclase (type I), which was first cloned from bovine brain, has been implicated in learning and memory. The objective of this study was to clone and determine the chromosomal localization of human fetal brain type I adenylyl cyclase. A 3.8-kb cDNA clone was isolated that contained sequence coinciding with the 3′ end 2553 nucleotides of the bovine open reading frame. This clone shows 87% nucleotide and 92% translated amino acid sequence identity to the bovine clone. The most significant sequence differences were in the carboxy-terminal 100 amino acid residues. This region contains one of several possible calmodulin binding domains and the only putative cAMP-dependent protein kinase A phosphorylation site. A chimera was constructed that contained the 5′ half of the bovine type I adenylyl cyclase and the 3′ half of the human type I adenylyl cyclase. The activity of the chimeric gene product and its sensitivity to calmodulin and calcium were indistinguishable from those of the bovine type I adenylyl cyclase.
In situ hybridization was used to localize the human type I adenylyl cyclase gene to the proximal portion of the short arm of chromosome 7.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1993.1213</identifier><identifier>PMID: 8314585</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adenylyl Cyclases - biosynthesis ; Adenylyl Cyclases - genetics ; Amino Acid Sequence ; Animals ; Biological and medical sciences ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Brain - embryology ; Brain - enzymology ; Cattle ; Chromosome Mapping ; Cloning, Molecular ; CYCLASES ; DNA HYBRIDIZATION ; DNA SEQUENCING ; DNA-CLONING ; Enzyme Induction ; Fundamental and applied biological sciences. Psychology ; GENES ; Genes. Genome ; GENETIC MAPPING ; HUMAN CHROMOSOME 7 ; Humans ; Membrane Proteins ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nerve Tissue Proteins - biosynthesis ; Nerve Tissue Proteins - genetics ; Sequence Alignment ; Sequence Homology, Amino Acid</subject><ispartof>Genomics, 1993-05, Vol.16 (2), p.473-478</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-2ffe8678d2f58be0cdd9b459afa6267ec5986e9f979770c9432319fa2b76cfe93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754383712139$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4782502$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8314585$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/28974$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Villacres, Enrique C.</creatorcontrib><creatorcontrib>Xia, Zhengui</creatorcontrib><creatorcontrib>Bookbinder, Louis H.</creatorcontrib><creatorcontrib>Edelhoff, Susanne</creatorcontrib><creatorcontrib>Disteche, Christine M.</creatorcontrib><creatorcontrib>Storm, Daniel R.</creatorcontrib><title>Cloning, Chromosomal Mapping, and Expression of Human Fetal Brain Type I Adenylyl Cyclase</title><title>Genomics</title><addtitle>Genomics</addtitle><description>The neural-specific calmodulin-sensitive adenylyl cyclase (type I), which was first cloned from bovine brain, has been implicated in learning and memory. The objective of this study was to clone and determine the chromosomal localization of human fetal brain type I adenylyl cyclase. A 3.8-kb cDNA clone was isolated that contained sequence coinciding with the 3′ end 2553 nucleotides of the bovine open reading frame. This clone shows 87% nucleotide and 92% translated amino acid sequence identity to the bovine clone. The most significant sequence differences were in the carboxy-terminal 100 amino acid residues. This region contains one of several possible calmodulin binding domains and the only putative cAMP-dependent protein kinase A phosphorylation site. A chimera was constructed that contained the 5′ half of the bovine type I adenylyl cyclase and the 3′ half of the human type I adenylyl cyclase. The activity of the chimeric gene product and its sensitivity to calmodulin and calcium were indistinguishable from those of the bovine type I adenylyl cyclase.
In situ hybridization was used to localize the human type I adenylyl cyclase gene to the proximal portion of the short arm of chromosome 7.</description><subject>Adenylyl Cyclases - biosynthesis</subject><subject>Adenylyl Cyclases - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Brain - embryology</subject><subject>Brain - enzymology</subject><subject>Cattle</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>CYCLASES</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA SEQUENCING</subject><subject>DNA-CLONING</subject><subject>Enzyme Induction</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENES</subject><subject>Genes. Genome</subject><subject>GENETIC MAPPING</subject><subject>HUMAN CHROMOSOME 7</subject><subject>Humans</subject><subject>Membrane Proteins</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi1EVbalV25IPiBOZPFHHNvHErW0Uisu5dCT5XXGrVFiBzuLyL8n6a564zTSzDOjeR-EPlCypYQ0X58gpi3Vmm8po_wN2lCidKWaunmLNkQpVUlR83forJRfhBDNFTtFp4rTWiixQY9tn2KIT19w-5zTkEoabI_v7Ti-NG3s8NXfMUMpIUWcPL7ZDzbia5gW7Fu2IeKHeQR8iy87iHM_97idXW8LvEcn3vYFLo71HP28vnpob6q7H99v28u7ynHNp4p5D6qRqmNeqB0Q13V6VwttvW1YI8EJrRrQXkstJXG65oxT7S3bycZ50Pwc4cPdVKZgigsTuGeXYgQ3Gaa0rBfk8wEZc_q9hzKZIRQHfW8jpH0xUijCtCALuD2ALqdSMngz5jDYPBtKzKrbrLrNqtusupeFj8fL-90A3St-9LvMPx3ntjjb-2yjC-UVq6VigrAFUwcMFlF_AuQ1B0QHXchrjC6F_33wD55Rmp0</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>Villacres, Enrique C.</creator><creator>Xia, Zhengui</creator><creator>Bookbinder, Louis H.</creator><creator>Edelhoff, Susanne</creator><creator>Disteche, Christine M.</creator><creator>Storm, Daniel R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19930501</creationdate><title>Cloning, Chromosomal Mapping, and Expression of Human Fetal Brain Type I Adenylyl Cyclase</title><author>Villacres, Enrique C. ; Xia, Zhengui ; Bookbinder, Louis H. ; Edelhoff, Susanne ; Disteche, Christine M. ; Storm, Daniel R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-2ffe8678d2f58be0cdd9b459afa6267ec5986e9f979770c9432319fa2b76cfe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenylyl Cyclases - biosynthesis</topic><topic>Adenylyl Cyclases - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Brain - embryology</topic><topic>Brain - enzymology</topic><topic>Cattle</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>CYCLASES</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA SEQUENCING</topic><topic>DNA-CLONING</topic><topic>Enzyme Induction</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENES</topic><topic>Genes. Genome</topic><topic>GENETIC MAPPING</topic><topic>HUMAN CHROMOSOME 7</topic><topic>Humans</topic><topic>Membrane Proteins</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Villacres, Enrique C.</creatorcontrib><creatorcontrib>Xia, Zhengui</creatorcontrib><creatorcontrib>Bookbinder, Louis H.</creatorcontrib><creatorcontrib>Edelhoff, Susanne</creatorcontrib><creatorcontrib>Disteche, Christine M.</creatorcontrib><creatorcontrib>Storm, Daniel R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Villacres, Enrique C.</au><au>Xia, Zhengui</au><au>Bookbinder, Louis H.</au><au>Edelhoff, Susanne</au><au>Disteche, Christine M.</au><au>Storm, Daniel R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, Chromosomal Mapping, and Expression of Human Fetal Brain Type I Adenylyl Cyclase</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1993-05-01</date><risdate>1993</risdate><volume>16</volume><issue>2</issue><spage>473</spage><epage>478</epage><pages>473-478</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>The neural-specific calmodulin-sensitive adenylyl cyclase (type I), which was first cloned from bovine brain, has been implicated in learning and memory. The objective of this study was to clone and determine the chromosomal localization of human fetal brain type I adenylyl cyclase. A 3.8-kb cDNA clone was isolated that contained sequence coinciding with the 3′ end 2553 nucleotides of the bovine open reading frame. This clone shows 87% nucleotide and 92% translated amino acid sequence identity to the bovine clone. The most significant sequence differences were in the carboxy-terminal 100 amino acid residues. This region contains one of several possible calmodulin binding domains and the only putative cAMP-dependent protein kinase A phosphorylation site. A chimera was constructed that contained the 5′ half of the bovine type I adenylyl cyclase and the 3′ half of the human type I adenylyl cyclase. The activity of the chimeric gene product and its sensitivity to calmodulin and calcium were indistinguishable from those of the bovine type I adenylyl cyclase.
In situ hybridization was used to localize the human type I adenylyl cyclase gene to the proximal portion of the short arm of chromosome 7.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8314585</pmid><doi>10.1006/geno.1993.1213</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics, 1993-05, Vol.16 (2), p.473-478 |
| issn | 0888-7543 1089-8646 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75802950 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenylyl Cyclases - biosynthesis Adenylyl Cyclases - genetics Amino Acid Sequence Animals Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES Brain - embryology Brain - enzymology Cattle Chromosome Mapping Cloning, Molecular CYCLASES DNA HYBRIDIZATION DNA SEQUENCING DNA-CLONING Enzyme Induction Fundamental and applied biological sciences. Psychology GENES Genes. Genome GENETIC MAPPING HUMAN CHROMOSOME 7 Humans Membrane Proteins Molecular and cellular biology Molecular genetics Molecular Sequence Data Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Sequence Alignment Sequence Homology, Amino Acid |
| title | Cloning, Chromosomal Mapping, and Expression of Human Fetal Brain Type I Adenylyl Cyclase |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T22%3A47%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning,%20Chromosomal%20Mapping,%20and%20Expression%20of%20Human%20Fetal%20Brain%20Type%20I%20Adenylyl%20Cyclase&rft.jtitle=Genomics&rft.au=Villacres,%20Enrique%20C.&rft.date=1993-05-01&rft.volume=16&rft.issue=2&rft.spage=473&rft.epage=478&rft.pages=473-478&rft.issn=0888-7543&rft.eissn=1089-8646&rft_id=info:doi/10.1006/geno.1993.1213&rft_dat=%3Cproquest_osti_%3E75802950%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75802950&rft_id=info:pmid/8314585&rft_els_id=S0888754383712139&rfr_iscdi=true |