Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification
Cell culture of Taxus spp. represents a potential alternative source of taxol and related taxanes used in cancer chemotherapy. We have analyzed the effect of different culture media components on growth and production of taxol in developing callus cultures of T cuspidata. Several sequential modifica...
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| Veröffentlicht in: | Bio/Technology 1993-06, Vol.11 (6), p.731-734 |
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| creator | Fett-Neto, A.G Melanson, S.J Sakata, K DiCosmo, F |
| description | Cell culture of Taxus spp. represents a potential alternative source of taxol and related taxanes used in cancer chemotherapy. We have analyzed the effect of different culture media components on growth and production of taxol in developing callus cultures of T cuspidata. Several sequential modifications were made to the basal B5 medium, which included addition and/or variation in the concentration of sucrose, B5 organic supplements, gibberellic acid, 36 combinations of 2,4-D/kinetin ratios, media salts and organic supplements, phenylalanine, casein hydrolysate and medium pH. The experiments were conducted during a 55 day-growth period followed by taxane extraction and analysis. Significant increases in taxol yield and growth over basal medium grown calli were observed with some of the modified media |
| doi_str_mv | 10.1038/nbt0693-731 |
| format | Article |
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We have analyzed the effect of different culture media components on growth and production of taxol in developing callus cultures of T cuspidata. Several sequential modifications were made to the basal B5 medium, which included addition and/or variation in the concentration of sucrose, B5 organic supplements, gibberellic acid, 36 combinations of 2,4-D/kinetin ratios, media salts and organic supplements, phenylalanine, casein hydrolysate and medium pH. The experiments were conducted during a 55 day-growth period followed by taxane extraction and analysis. 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We have analyzed the effect of different culture media components on growth and production of taxol in developing callus cultures of T cuspidata. Several sequential modifications were made to the basal B5 medium, which included addition and/or variation in the concentration of sucrose, B5 organic supplements, gibberellic acid, 36 combinations of 2,4-D/kinetin ratios, media salts and organic supplements, phenylalanine, casein hydrolysate and medium pH. The experiments were conducted during a 55 day-growth period followed by taxane extraction and analysis. Significant increases in taxol yield and growth over basal medium grown calli were observed with some of the modified media</description><subject>2,4-D</subject><subject>2,4-Dichlorophenoxyacetic Acid</subject><subject>ACIDO GIBERELICO</subject><subject>Adenine - analogs & derivatives</subject><subject>Agriculture</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>Biotechnology</subject><subject>CAL</subject><subject>CALLO</subject><subject>Caseins</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>CULTIVO DE TEJIDOS</subject><subject>CULTURE DE TISSU</subject><subject>Culture Media</subject><subject>DITERPENOIDE</subject><subject>DITERPENOS</subject><subject>Eukaryotic cell cultures</subject><subject>FENILALANINA</subject><subject>Fundamental and applied biological sciences. 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Technologies</subject><subject>MILIEU DE CULTURE</subject><subject>Miscellaneous</subject><subject>Paclitaxel - biosynthesis</subject><subject>PHENYLALANINE</subject><subject>Plant Cells</subject><subject>Plant cells and fungal cells</subject><subject>Plants - metabolism</subject><subject>Protein Hydrolysates</subject><subject>SACCHAROSE</subject><subject>SUCROSA</subject><subject>Sucrose</subject><subject>TAXUS</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtrFTEAhYMo9Xp15U4QshA3Opp3MkspPgoFF7bgLmTyuKbMJGMyU3v_val36M5VCOfjhPMFgJcYfcCIqo9pWJDoaScpfgR2hFLcUaHYY7BDktKOEPLzKXhW6w1CTArCzsCZlIJTxHYgXkxzybfewUPJf5Zf0CQHF3OXR3iMfnQwJuj8rR_zHNMBWjOOEeYAr8zdWqFd6xydWQwcjnDyLq4TtHmac41LzAlO2cUQrbm_PAdPghmrf7Gde3D95fPV-bfu8vvXi_NPl51lVC0dkwxLQzjjve2JCINqewQyYSCIO86xE4wNQTAiXPCceisYZ6aX3ggVKKZ78PbU23b9Xn1d9BSr9eNoks9r1ZIrhLmQDXx3Am3JtRYf9FziZMpRY6TvxepNrJb_al9vtevQlj6wm8mWv9lyU5ulUEyysT5gTCEq22_swfsTVluSDr7om7yW1IT859VXJzyYrM2htMbrHz0jSipO_wLWVpgV</recordid><startdate>19930601</startdate><enddate>19930601</enddate><creator>Fett-Neto, A.G</creator><creator>Melanson, S.J</creator><creator>Sakata, K</creator><creator>DiCosmo, F</creator><general>Nature Publishing Group US</general><general>Nature Publications</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930601</creationdate><title>Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification</title><author>Fett-Neto, A.G ; Melanson, S.J ; Sakata, K ; DiCosmo, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-47417a25459c926fb833160afb205d551d644bf6426dfe53ec6454a97ea68f313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>2,4-D</topic><topic>2,4-Dichlorophenoxyacetic Acid</topic><topic>ACIDO GIBERELICO</topic><topic>Adenine - analogs & derivatives</topic><topic>Agriculture</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>Biotechnology</topic><topic>CAL</topic><topic>CALLO</topic><topic>Caseins</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>CULTIVO DE TEJIDOS</topic><topic>CULTURE DE TISSU</topic><topic>Culture Media</topic><topic>DITERPENOIDE</topic><topic>DITERPENOS</topic><topic>Eukaryotic cell cultures</topic><topic>FENILALANINA</topic><topic>Fundamental and applied biological sciences. 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Technologies</topic><topic>MILIEU DE CULTURE</topic><topic>Miscellaneous</topic><topic>Paclitaxel - biosynthesis</topic><topic>PHENYLALANINE</topic><topic>Plant Cells</topic><topic>Plant cells and fungal cells</topic><topic>Plants - metabolism</topic><topic>Protein Hydrolysates</topic><topic>SACCHAROSE</topic><topic>SUCROSA</topic><topic>Sucrose</topic><topic>TAXUS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fett-Neto, A.G</creatorcontrib><creatorcontrib>Melanson, S.J</creatorcontrib><creatorcontrib>Sakata, K</creatorcontrib><creatorcontrib>DiCosmo, F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fett-Neto, A.G</au><au>Melanson, S.J</au><au>Sakata, K</au><au>DiCosmo, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Biotechnology (N Y)</addtitle><date>1993-06-01</date><risdate>1993</risdate><volume>11</volume><issue>6</issue><spage>731</spage><epage>734</epage><pages>731-734</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>Cell culture of Taxus spp. represents a potential alternative source of taxol and related taxanes used in cancer chemotherapy. We have analyzed the effect of different culture media components on growth and production of taxol in developing callus cultures of T cuspidata. Several sequential modifications were made to the basal B5 medium, which included addition and/or variation in the concentration of sucrose, B5 organic supplements, gibberellic acid, 36 combinations of 2,4-D/kinetin ratios, media salts and organic supplements, phenylalanine, casein hydrolysate and medium pH. The experiments were conducted during a 55 day-growth period followed by taxane extraction and analysis. Significant increases in taxol yield and growth over basal medium grown calli were observed with some of the modified media</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>7765304</pmid><doi>10.1038/nbt0693-731</doi><tpages>4</tpages></addata></record> |
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| subjects | 2,4-D 2,4-Dichlorophenoxyacetic Acid ACIDO GIBERELICO Adenine - analogs & derivatives Agriculture Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine BIOSINTESIS BIOSYNTHESE Biotechnology CAL CALLO Caseins Cell Division Cells, Cultured CULTIVO DE TEJIDOS CULTURE DE TISSU Culture Media DITERPENOIDE DITERPENOS Eukaryotic cell cultures FENILALANINA Fundamental and applied biological sciences. Psychology GIBBERELLINE Gibberellins Hydrogen-Ion Concentration Kinetin Life Sciences MEDIO DE CULTIVO Methods. Procedures. Technologies MILIEU DE CULTURE Miscellaneous Paclitaxel - biosynthesis PHENYLALANINE Plant Cells Plant cells and fungal cells Plants - metabolism Protein Hydrolysates SACCHAROSE SUCROSA Sucrose TAXUS |
| title | Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification |
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