Isolation of Two Cell Lines from a Human Malignant Glioma Specimen Differing in Sensitivity to Radiation and Chemotherapeutic Drugs
Two aneuploid cell lines which differ in their inherent sensitivity to ionizing radiation and chemotherapeutic agents were established concurrently from a single tumor specimen obtained from a patient with glioblastoma. M059J cells are approximately 30-fold more sensitive to radiation than are M059K...
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| Veröffentlicht in: | Radiation research 1993-06, Vol.134 (3), p.349-354 |
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| container_title | Radiation research |
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| creator | Allalunis-Turner, M. J. Barron, G. M. Day, R. S. Dobler, K. D. Mirzayans, R. |
| description | Two aneuploid cell lines which differ in their inherent sensitivity to ionizing radiation and chemotherapeutic agents were established concurrently from a single tumor specimen obtained from a patient with glioblastoma. M059J cells are approximately 30-fold more sensitive to radiation than are M059K cells (surviving fractions at 2 Gy were 0.02 and 0.64, respectively). This relative difference in radiation sensitivity has remained a stable feature of the cell lines during 2 years in continuous culture. In addition, cells of the M059J line are more sensitive than those of the M059K line to the cytotoxic effects of bleomycin, N,N-bis(2-chloroethyl)-N-nitrosourea, and nitrogen mustard. These cell lines may prove to provide a useful model system for evaluating the cellular and molecular processes which confer resistance or sensitivity in cancer treatment. |
| doi_str_mv | 10.2307/3578196 |
| format | Article |
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J. ; Barron, G. M. ; Day, R. S. ; Dobler, K. D. ; Mirzayans, R.</creator><creatorcontrib>Allalunis-Turner, M. J. ; Barron, G. M. ; Day, R. S. ; Dobler, K. D. ; Mirzayans, R.</creatorcontrib><description>Two aneuploid cell lines which differ in their inherent sensitivity to ionizing radiation and chemotherapeutic agents were established concurrently from a single tumor specimen obtained from a patient with glioblastoma. M059J cells are approximately 30-fold more sensitive to radiation than are M059K cells (surviving fractions at 2 Gy were 0.02 and 0.64, respectively). This relative difference in radiation sensitivity has remained a stable feature of the cell lines during 2 years in continuous culture. In addition, cells of the M059J line are more sensitive than those of the M059K line to the cytotoxic effects of bleomycin, N,N-bis(2-chloroethyl)-N-nitrosourea, and nitrogen mustard. These cell lines may prove to provide a useful model system for evaluating the cellular and molecular processes which confer resistance or sensitivity in cancer treatment.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3578196</identifier><identifier>PMID: 8316628</identifier><identifier>CODEN: RAREAE</identifier><language>eng</language><publisher>Oak Brook, Il: Academic Press, Inc</publisher><subject>Adult ; Aneuploidy ; Antineoplastic Agents - therapeutic use ; Biological and medical sciences ; Biological effects of radiation ; Cell culture techniques ; Cell lines ; CHO cells ; DNA ; Drug Resistance - physiology ; Fundamental and applied biological sciences. 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J.</creatorcontrib><creatorcontrib>Barron, G. M.</creatorcontrib><creatorcontrib>Day, R. S.</creatorcontrib><creatorcontrib>Dobler, K. D.</creatorcontrib><creatorcontrib>Mirzayans, R.</creatorcontrib><title>Isolation of Two Cell Lines from a Human Malignant Glioma Specimen Differing in Sensitivity to Radiation and Chemotherapeutic Drugs</title><title>Radiation research</title><addtitle>Radiat Res</addtitle><description>Two aneuploid cell lines which differ in their inherent sensitivity to ionizing radiation and chemotherapeutic agents were established concurrently from a single tumor specimen obtained from a patient with glioblastoma. M059J cells are approximately 30-fold more sensitive to radiation than are M059K cells (surviving fractions at 2 Gy were 0.02 and 0.64, respectively). This relative difference in radiation sensitivity has remained a stable feature of the cell lines during 2 years in continuous culture. In addition, cells of the M059J line are more sensitive than those of the M059K line to the cytotoxic effects of bleomycin, N,N-bis(2-chloroethyl)-N-nitrosourea, and nitrogen mustard. These cell lines may prove to provide a useful model system for evaluating the cellular and molecular processes which confer resistance or sensitivity in cancer treatment.</description><subject>Adult</subject><subject>Aneuploidy</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Cell culture techniques</subject><subject>Cell lines</subject><subject>CHO cells</subject><subject>DNA</subject><subject>Drug Resistance - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glioma</subject><subject>Glioma - physiopathology</subject><subject>Humans</subject><subject>Ionizing radiations</subject><subject>Male</subject><subject>Neuroglia</subject><subject>Radiation tolerance</subject><subject>Radiation Tolerance - physiology</subject><subject>Space life sciences</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Tumor cell line</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumors</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1r3DAQxUVJSbZp6V8Q0CEkJzf6WFnysezmC7YEmvRsRvZ4o2BLW0lOyLn_eB3WhF56egzvx5uZR8hXzr4JyfSFVNrwqvxAFrySplBLtjwgC8akLLQy-oh8SumJTTMvq0NyaCYthVmQP7cp9JBd8DR09OEl0BX2Pd04j4l2MQwU6M04gKc_oHdbDz7T696FAej9Dhs3oKdr13UYnd9S5-k9-uSye3b5leZAf0Lr9vHgW7p6xCHkR4ywwzG7hq7juE2fyccO-oRfZj0mv64uH1Y3xebu-nb1fVM0kqtcGKNsa62w1oBo5XS-RmaUsFgpYVAKy6tGGq4VGq2h7UrJtZEClGbailIek7N97i6G3yOmXA8uNdO74DGMqdZKV1qWb-D5HmxiSCliV--iGyC-1pzVb3XXc90TeTJHjnbA9p2b-53809mH1EDfRfCNS--Y1EtVsn-wp5RD_O-2v3fjkfY</recordid><startdate>199306</startdate><enddate>199306</enddate><creator>Allalunis-Turner, M. 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D. ; Mirzayans, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-885bdbb2bb8a2d36287e0852be9528e32b19c38175e877adf6317832a5707b263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adult</topic><topic>Aneuploidy</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Cell culture techniques</topic><topic>Cell lines</topic><topic>CHO cells</topic><topic>DNA</topic><topic>Drug Resistance - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glioma</topic><topic>Glioma - physiopathology</topic><topic>Humans</topic><topic>Ionizing radiations</topic><topic>Male</topic><topic>Neuroglia</topic><topic>Radiation tolerance</topic><topic>Radiation Tolerance - physiology</topic><topic>Space life sciences</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Tumor cell line</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allalunis-Turner, M. J.</creatorcontrib><creatorcontrib>Barron, G. M.</creatorcontrib><creatorcontrib>Day, R. S.</creatorcontrib><creatorcontrib>Dobler, K. 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D.</au><au>Mirzayans, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Two Cell Lines from a Human Malignant Glioma Specimen Differing in Sensitivity to Radiation and Chemotherapeutic Drugs</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>1993-06</date><risdate>1993</risdate><volume>134</volume><issue>3</issue><spage>349</spage><epage>354</epage><pages>349-354</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><coden>RAREAE</coden><abstract>Two aneuploid cell lines which differ in their inherent sensitivity to ionizing radiation and chemotherapeutic agents were established concurrently from a single tumor specimen obtained from a patient with glioblastoma. M059J cells are approximately 30-fold more sensitive to radiation than are M059K cells (surviving fractions at 2 Gy were 0.02 and 0.64, respectively). This relative difference in radiation sensitivity has remained a stable feature of the cell lines during 2 years in continuous culture. In addition, cells of the M059J line are more sensitive than those of the M059K line to the cytotoxic effects of bleomycin, N,N-bis(2-chloroethyl)-N-nitrosourea, and nitrogen mustard. These cell lines may prove to provide a useful model system for evaluating the cellular and molecular processes which confer resistance or sensitivity in cancer treatment.</abstract><cop>Oak Brook, Il</cop><pub>Academic Press, Inc</pub><pmid>8316628</pmid><doi>10.2307/3578196</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0033-7587 |
| ispartof | Radiation research, 1993-06, Vol.134 (3), p.349-354 |
| issn | 0033-7587 1938-5404 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy |
| subjects | Adult Aneuploidy Antineoplastic Agents - therapeutic use Biological and medical sciences Biological effects of radiation Cell culture techniques Cell lines CHO cells DNA Drug Resistance - physiology Fundamental and applied biological sciences. Psychology Glioma Glioma - physiopathology Humans Ionizing radiations Male Neuroglia Radiation tolerance Radiation Tolerance - physiology Space life sciences Tissues, organs and organisms biophysics Tumor cell line Tumor Cells, Cultured - drug effects Tumors |
| title | Isolation of Two Cell Lines from a Human Malignant Glioma Specimen Differing in Sensitivity to Radiation and Chemotherapeutic Drugs |
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