Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: a combination of gene transfer and microcell fusion
We have used a combination of chromosome-mediated gene transfer and microcell fusion techniques to transfer Chinese hamster chromosome 1 to mouse cells. Microcell hybrids containing a single hamster chromosome were analyzed to map genes on this chromosome. We have confirmed the assignment of seven m...
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| Veröffentlicht in: | Somatic cell and molecular genetics 1984-11, Vol.10 (6), p.547-559 |
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| creator | VEENA DHAR SEARLE, B. M ATHWAL, R. S |
| description | We have used a combination of chromosome-mediated gene transfer and microcell fusion techniques to transfer Chinese hamster chromosome 1 to mouse cells. Microcell hybrids containing a single hamster chromosome were analyzed to map genes on this chromosome. We have confirmed the assignment of seven markers (GSR, NP, EST-D, ADK, PEP-S, PGM2, and PEP-B) to hamster chromosome 1. Segregation among the linked markers was induced by X irradiation followed by selection for the retention or loss of human hprt. Cosegregation of markers in independent subclones made it possible to determine the gene order for the seven loci. The gene order proposed for these loci is as follows: pter-GSR-NP-EST-D-ADK-(PEP-S, PGM2)-PEP-B-qter. In addition GSR, NP, EST-D, and ADK have been assigned to pter-1q12; PEP-S and PGM2 to 1q12-1q21, and PEP-B to 1q32-1qter. These regional assignments and gene order on chromosome 1 have provided the information relevant to the linkages conserved between Chinese hamster, mouse, and man. |
| doi_str_mv | 10.1007/BF01535220 |
| format | Article |
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M ; ATHWAL, R. S</creator><creatorcontrib>VEENA DHAR ; SEARLE, B. M ; ATHWAL, R. S</creatorcontrib><description>We have used a combination of chromosome-mediated gene transfer and microcell fusion techniques to transfer Chinese hamster chromosome 1 to mouse cells. Microcell hybrids containing a single hamster chromosome were analyzed to map genes on this chromosome. We have confirmed the assignment of seven markers (GSR, NP, EST-D, ADK, PEP-S, PGM2, and PEP-B) to hamster chromosome 1. Segregation among the linked markers was induced by X irradiation followed by selection for the retention or loss of human hprt. Cosegregation of markers in independent subclones made it possible to determine the gene order for the seven loci. The gene order proposed for these loci is as follows: pter-GSR-NP-EST-D-ADK-(PEP-S, PGM2)-PEP-B-qter. In addition GSR, NP, EST-D, and ADK have been assigned to pter-1q12; PEP-S and PGM2 to 1q12-1q21, and PEP-B to 1q32-1qter. These regional assignments and gene order on chromosome 1 have provided the information relevant to the linkages conserved between Chinese hamster, mouse, and man.</description><identifier>ISSN: 0740-7750</identifier><identifier>EISSN: 1572-9931</identifier><identifier>DOI: 10.1007/BF01535220</identifier><identifier>PMID: 6239397</identifier><identifier>CODEN: SCMGDN</identifier><language>eng</language><publisher>London: Plenum</publisher><subject>Adenosine Kinase - genetics ; Animals ; Biological and medical sciences ; Biotechnology ; Carboxylesterase ; Carboxylic Ester Hydrolases - genetics ; Chromosome Mapping ; Chromosomes - physiology ; Cricetinae - genetics ; Cricetulus - genetics ; Cytogenetics ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic engineering ; Genetic Linkage ; Genetic technics ; Genetics of eukaryotes. Biological and molecular evolution ; Glutathione Reductase - genetics ; Methods. Procedures. Technologies ; Phosphoglucomutase - genetics ; Purine-Nucleoside Phosphorylase - genetics ; Vectors (cloning, transfer, expression). Insertion sequences and transposons ; Vertebrata</subject><ispartof>Somatic cell and molecular genetics, 1984-11, Vol.10 (6), p.547-559</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-206d910fe14d3f73943f37c0583cc7bee564de69675c22c3939a1ac5c53126903</citedby><cites>FETCH-LOGICAL-c342t-206d910fe14d3f73943f37c0583cc7bee564de69675c22c3939a1ac5c53126903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9168940$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6239397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VEENA DHAR</creatorcontrib><creatorcontrib>SEARLE, B. M</creatorcontrib><creatorcontrib>ATHWAL, R. S</creatorcontrib><title>Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: a combination of gene transfer and microcell fusion</title><title>Somatic cell and molecular genetics</title><addtitle>Somat Cell Mol Genet</addtitle><description>We have used a combination of chromosome-mediated gene transfer and microcell fusion techniques to transfer Chinese hamster chromosome 1 to mouse cells. Microcell hybrids containing a single hamster chromosome were analyzed to map genes on this chromosome. We have confirmed the assignment of seven markers (GSR, NP, EST-D, ADK, PEP-S, PGM2, and PEP-B) to hamster chromosome 1. Segregation among the linked markers was induced by X irradiation followed by selection for the retention or loss of human hprt. Cosegregation of markers in independent subclones made it possible to determine the gene order for the seven loci. The gene order proposed for these loci is as follows: pter-GSR-NP-EST-D-ADK-(PEP-S, PGM2)-PEP-B-qter. In addition GSR, NP, EST-D, and ADK have been assigned to pter-1q12; PEP-S and PGM2 to 1q12-1q21, and PEP-B to 1q32-1qter. These regional assignments and gene order on chromosome 1 have provided the information relevant to the linkages conserved between Chinese hamster, mouse, and man.</description><subject>Adenosine Kinase - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carboxylesterase</subject><subject>Carboxylic Ester Hydrolases - genetics</subject><subject>Chromosome Mapping</subject><subject>Chromosomes - physiology</subject><subject>Cricetinae - genetics</subject><subject>Cricetulus - genetics</subject><subject>Cytogenetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic Linkage</subject><subject>Genetic technics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Glutathione Reductase - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Phosphoglucomutase - genetics</subject><subject>Purine-Nucleoside Phosphorylase - genetics</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><subject>Vertebrata</subject><issn>0740-7750</issn><issn>1572-9931</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbFuFDEQhi0ESo5LGvpILhAF0sLYXttnunAigBSJJtQrn3d8t9F6nXj2Cp6CV8arHKGksjT_p8_-PYy9EfBBANiPn29AaKWlhBdsJbSVjXNKvGQrsC001mo4Z6-J7gFgs1H6jJ0ZqZxydsV-3xU_UcTCc-TbwzAhIT_4RHMdhUPJKVNOyAWfM0_5WNOA40jcTz0vuB_y5EfuiYb9lHCaF43le6yeT9zzkNNumPxcsSVZ5nz-e-OiSEMoeTHyeKRKXbBX0Y-El6dzzX7efLnbfmtuf3z9vr2-bYJq5dxIML0TEFG0vYpWuVZFZQPojQrB7hC1aXs0zlgdpAxLWS980EErIY0DtWbvnrwPJT8ekeYuDbS8w09YW3ZWW6e1k_8FRSuMEU5U8P0TWAsRFYzdQxmSL786Ad2ypu7fmip8dbIedwn7Z_S0l5q_PeWegh9j_bIw0DPmhNm4FtQfYb6ZyA</recordid><startdate>198411</startdate><enddate>198411</enddate><creator>VEENA DHAR</creator><creator>SEARLE, B. M</creator><creator>ATHWAL, R. S</creator><general>Plenum</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198411</creationdate><title>Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: a combination of gene transfer and microcell fusion</title><author>VEENA DHAR ; SEARLE, B. M ; ATHWAL, R. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-206d910fe14d3f73943f37c0583cc7bee564de69675c22c3939a1ac5c53126903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Adenosine Kinase - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carboxylesterase</topic><topic>Carboxylic Ester Hydrolases - genetics</topic><topic>Chromosome Mapping</topic><topic>Chromosomes - physiology</topic><topic>Cricetinae - genetics</topic><topic>Cricetulus - genetics</topic><topic>Cytogenetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic Linkage</topic><topic>Genetic technics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Glutathione Reductase - genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Phosphoglucomutase - genetics</topic><topic>Purine-Nucleoside Phosphorylase - genetics</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VEENA DHAR</creatorcontrib><creatorcontrib>SEARLE, B. M</creatorcontrib><creatorcontrib>ATHWAL, R. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Somatic cell and molecular genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VEENA DHAR</au><au>SEARLE, B. M</au><au>ATHWAL, R. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: a combination of gene transfer and microcell fusion</atitle><jtitle>Somatic cell and molecular genetics</jtitle><addtitle>Somat Cell Mol Genet</addtitle><date>1984-11</date><risdate>1984</risdate><volume>10</volume><issue>6</issue><spage>547</spage><epage>559</epage><pages>547-559</pages><issn>0740-7750</issn><eissn>1572-9931</eissn><coden>SCMGDN</coden><abstract>We have used a combination of chromosome-mediated gene transfer and microcell fusion techniques to transfer Chinese hamster chromosome 1 to mouse cells. Microcell hybrids containing a single hamster chromosome were analyzed to map genes on this chromosome. We have confirmed the assignment of seven markers (GSR, NP, EST-D, ADK, PEP-S, PGM2, and PEP-B) to hamster chromosome 1. Segregation among the linked markers was induced by X irradiation followed by selection for the retention or loss of human hprt. Cosegregation of markers in independent subclones made it possible to determine the gene order for the seven loci. The gene order proposed for these loci is as follows: pter-GSR-NP-EST-D-ADK-(PEP-S, PGM2)-PEP-B-qter. In addition GSR, NP, EST-D, and ADK have been assigned to pter-1q12; PEP-S and PGM2 to 1q12-1q21, and PEP-B to 1q32-1qter. These regional assignments and gene order on chromosome 1 have provided the information relevant to the linkages conserved between Chinese hamster, mouse, and man.</abstract><cop>London</cop><cop>New York, NY</cop><pub>Plenum</pub><pmid>6239397</pmid><doi>10.1007/BF01535220</doi><tpages>13</tpages></addata></record> |
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| subjects | Adenosine Kinase - genetics Animals Biological and medical sciences Biotechnology Carboxylesterase Carboxylic Ester Hydrolases - genetics Chromosome Mapping Chromosomes - physiology Cricetinae - genetics Cricetulus - genetics Cytogenetics Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genetic Linkage Genetic technics Genetics of eukaryotes. Biological and molecular evolution Glutathione Reductase - genetics Methods. Procedures. Technologies Phosphoglucomutase - genetics Purine-Nucleoside Phosphorylase - genetics Vectors (cloning, transfer, expression). Insertion sequences and transposons Vertebrata |
| title | Transfer of Chinese hamster chromosome 1 to mouse cells and regional assignment of 7 genes: a combination of gene transfer and microcell fusion |
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