High-Level Expression, Purification, and Renaturation of Recombinant Murine Interleukin-2 from Escherichia coli

A murine interleukin-2 (mIL-2)-encoding cDNA, isolated from a stimulated EL4 mRNA library, was used to construct several expression plasmids directing synthesis of the mature protein in Escherichia coli. The expression was under control of either the P Trp or the P L promoter. Using these systems, a...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Protein expression and purification 1993-06, Vol.4 (3), p.240-246
Hauptverfasser:	Guisez, Y., Demolder, J., Mertens, N., Raeymaekers, A., Plaetinck, G., Robbens, J., Vandekerckhove, J., Remaut, E., Fiers, W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Escherichia coli - genetics Gene Expression Inclusion Bodies Interleukin-2 - biosynthesis Interleukin-2 - genetics Interleukin-2 - isolation & purification Mice Molecular Sequence Data Plasmids - genetics Protein Denaturation Protein Folding Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Solubility
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!