The carboxyl-terminal domain of human poly(ADP-ribose) polymerase. Overproduction in Escherichia coli, large scale purification, and characterization

The cDNA encoding the carboxyl-terminal 40-kDa domain of human poly(ADP-ribose) polymerase was inserted into an expression vector. The recombinant protein was overproduced in Escherichia coli, and purified to homogeneity. The 40-kDa domain had the same affinity (Km) for NAD+ as the full-length enzym...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	J Biol Chem 1993-06, Vol.268 (18), p.13454-13461
Hauptverfasser:	SIMONIN, F, HÖFFERER, L, PANZETER, P. L, MULLER, S, DE MURCIA, G, ALTHAUS, F. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biochemistry, Molecular Biology Biological and medical sciences Catalysis Chromatography, Ion Exchange Cloning, Molecular DNA DNA - metabolism Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Escherichia coli Fundamental and applied biological sciences. Psychology Humans Life Sciences Molecular Sequence Data NAD NAD - metabolism NAD+ Nucleosidase NAD+ Nucleosidase - metabolism Poly(ADP-ribose) Polymerases Poly(ADP-ribose) Polymerases - biosynthesis Poly(ADP-ribose) Polymerases - genetics Poly(ADP-ribose) Polymerases - isolation & purification Poly(ADP-ribose) Polymerases - metabolism Structural Biology Transferases
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!