Activation of DnaA5 protein by GrpE and DnaK heat shock proteins in initiation of DNA replication in Escherichia coli

DnaA5 protein, inactive in replication systems dependent on purified enzymes, was activated by addition of a crude enzyme fraction. Based on this assay, two proteins in the crude enzyme fraction were identified that confer replication activity upon DnaA5 protein in a purified enzyme system. That one...

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Veröffentlicht in:	The Journal of biological chemistry 1993-06, Vol.268 (18), p.13137-13142
Hauptverfasser:	HUPP, T. R, KAGUNI, J. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Biological and medical sciences Chromatography, Gel DNA Replication DNA Topoisomerases, Type I - metabolism DNA, Bacterial - biosynthesis DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism DNA-Directed RNA Polymerases - metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli Escherichia coli - genetics Escherichia coli Proteins Fundamental and applied biological sciences. Psychology Heat-Shock Proteins - isolation & purification Heat-Shock Proteins - metabolism HSP70 Heat-Shock Proteins Molecular and cellular biology Molecular genetics Replication Ribonuclease H - metabolism
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