Proto-Oncogene of Genomic DNA, Related to the Human Epidermal Growth Factor Receptor (EGFR) Gene, from Clinically Normal Domestic Animals
Genomic DNAs of cattle, horses, pigs, dogs, cats and chickens were surveyed using Southern blot hybridization analysis, with a human EGFR cDNA fragment. Several bands with different numbers and molecular weights were observed under the condition of low stringency in the individual animal species. Th...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 1993/04/15, Vol.55(2), pp.319-321 |
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| container_title | Journal of Veterinary Medical Science |
| container_volume | 55 |
| creator | KAI, Kiyonori TATEYAMA, Susumu MIYOSHI, Noriyuki YAMAGUCHI, Ryoji UCHIDA, Kazuyuki ROSTAMI, Mina |
| description | Genomic DNAs of cattle, horses, pigs, dogs, cats and chickens were surveyed using Southern blot hybridization analysis, with a human EGFR cDNA fragment. Several bands with different numbers and molecular weights were observed under the condition of low stringency in the individual animal species. The bands showing DNA polymorphism were observed among bovine genomic PstI-digested DNAs from 4 individuals and EcoRI-digested genomic DNAs from 4 chickens. These results may provide basic data which are useful for analysis of tumorigenetic mechanisms in domestic animals. |
| doi_str_mv | 10.1292/jvms.55.319 |
| format | Article |
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Several bands with different numbers and molecular weights were observed under the condition of low stringency in the individual animal species. The bands showing DNA polymorphism were observed among bovine genomic PstI-digested DNAs from 4 individuals and EcoRI-digested genomic DNAs from 4 chickens. These results may provide basic data which are useful for analysis of tumorigenetic mechanisms in domestic animals.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.55.319</identifier><identifier>PMID: 8513016</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; Animals, Domestic ; Blood Cells - physiology ; Blotting, Southern ; Cats ; Cattle ; Chickens ; DNA - genetics ; DNA - isolation & purification ; DNA Probes ; Dogs ; epidermal growth factor receptor gene ; ErbB Receptors - genetics ; Female ; Horses ; Humans ; hybridization ; Male ; Placenta - physiology ; Pregnancy ; proto-oncogene ; Proto-Oncogenes ; Reference Values ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Swine ; Testis - physiology</subject><ispartof>Journal of Veterinary Medical Science, 1993/04/15, Vol.55(2), pp.319-321</ispartof><rights>The Japanese Society of Veterinary Science</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3419-9cce8426aab4587516335523d1d2c1c201484e18239ec17113364e622c4ef7b93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8513016$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KAI, Kiyonori</creatorcontrib><creatorcontrib>TATEYAMA, Susumu</creatorcontrib><creatorcontrib>MIYOSHI, Noriyuki</creatorcontrib><creatorcontrib>YAMAGUCHI, Ryoji</creatorcontrib><creatorcontrib>UCHIDA, Kazuyuki</creatorcontrib><creatorcontrib>ROSTAMI, Mina</creatorcontrib><title>Proto-Oncogene of Genomic DNA, Related to the Human Epidermal Growth Factor Receptor (EGFR) Gene, from Clinically Normal Domestic Animals</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Genomic DNAs of cattle, horses, pigs, dogs, cats and chickens were surveyed using Southern blot hybridization analysis, with a human EGFR cDNA fragment. Several bands with different numbers and molecular weights were observed under the condition of low stringency in the individual animal species. The bands showing DNA polymorphism were observed among bovine genomic PstI-digested DNAs from 4 individuals and EcoRI-digested genomic DNAs from 4 chickens. 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Vet. Med. Sci.</addtitle><date>1993-04</date><risdate>1993</risdate><volume>55</volume><issue>2</issue><spage>319</spage><epage>321</epage><pages>319-321</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Genomic DNAs of cattle, horses, pigs, dogs, cats and chickens were surveyed using Southern blot hybridization analysis, with a human EGFR cDNA fragment. Several bands with different numbers and molecular weights were observed under the condition of low stringency in the individual animal species. The bands showing DNA polymorphism were observed among bovine genomic PstI-digested DNAs from 4 individuals and EcoRI-digested genomic DNAs from 4 chickens. These results may provide basic data which are useful for analysis of tumorigenetic mechanisms in domestic animals.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>8513016</pmid><doi>10.1292/jvms.55.319</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 1993/04/15, Vol.55(2), pp.319-321 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75786375 |
| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Animals, Domestic Blood Cells - physiology Blotting, Southern Cats Cattle Chickens DNA - genetics DNA - isolation & purification DNA Probes Dogs epidermal growth factor receptor gene ErbB Receptors - genetics Female Horses Humans hybridization Male Placenta - physiology Pregnancy proto-oncogene Proto-Oncogenes Reference Values Restriction Mapping Sequence Homology, Nucleic Acid Swine Testis - physiology |
| title | Proto-Oncogene of Genomic DNA, Related to the Human Epidermal Growth Factor Receptor (EGFR) Gene, from Clinically Normal Domestic Animals |
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