Molecular Cloning and Sequencing of a Human Somatostatin Receptor, hSSTR4
We recently reported the cloning of a somatostatin receptor (SSTR) subtype from a rat genomic library designated rSSTR4. In the present study, we report the cloning of the human SSTR4 gene. A human genomic library was screened with a 1.2 kb fragment of rSSTR4 containing the full open reading frame a...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1993-06, Vol.193 (2), p.648-652 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 652 |
|---|---|
| container_issue | 2 |
| container_start_page | 648 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 193 |
| creator | Xu, Y. Song, J.F. Bruno, J.F. Berelowitz, M. |
| description | We recently reported the cloning of a somatostatin receptor (SSTR) subtype from a rat genomic library designated rSSTR4. In the present study, we report the cloning of the human SSTR4 gene. A human genomic library was screened with a 1.2 kb fragment of rSSTR4 containing the full open reading frame and a genomic clone, hSSTR4, was isolated. The deduced amino acid sequence of this clone encoded a protein of 388 amino acids and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SSTR4 sequences demonstrated 89% identity. In addition, the sequence of hSSTR4 shows 61%, 46%, and 47% sequence identity with previously identified isoforms hSSTR1, hSSTR2, and hSSTR3, respectively. |
| doi_str_mv | 10.1006/bbrc.1993.1673 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75783662</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X83716736</els_id><sourcerecordid>16746562</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-140985d18afcb029ab8d6e94b7c823461a97ab077c9ba20f4100478394fd63803</originalsourceid><addsrcrecordid>eNqFkM9LwzAUx4Moc_64ehN6EE92Jk2aNEcZ6gYTYZvgLbymqVbaZiat4H9v6sZu4unxeJ_v470PQhcETwjG_DbPnZ4QKemEcEEP0JhgieOEYHaIxjgQcSLJ6zE68f4DY0IYlyM0ylKSpJyN0fzJ1kb3NbhoWtu2at8iaItoZT570-qhtWUE0axvoI1WtoHO-g66qo2WRptNZ91N9L5arZfsDB2VUHtzvqun6OXhfj2dxYvnx_n0bhFrKmUXE4ZllhYkg1LnOJGQZwU3kuVCZwllnIAUkGMhtMwhwSULXzKRUcnKgtMM01N0vd27cTYc6TvVVF6buobW2N4rkQaa8-RfMPhiPP0FJ1tQO-u9M6XauKoB960IVoNkNUhWg-QhREPgcre5zxtT7PGd1TC_2s3Ba6hLB0Gl32NMCJpKHrBsi5mg66syTnldBeumqJzRnSps9dcFP1G9ldI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16746562</pqid></control><display><type>article</type><title>Molecular Cloning and Sequencing of a Human Somatostatin Receptor, hSSTR4</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Xu, Y. ; Song, J.F. ; Bruno, J.F. ; Berelowitz, M.</creator><creatorcontrib>Xu, Y. ; Song, J.F. ; Bruno, J.F. ; Berelowitz, M.</creatorcontrib><description>We recently reported the cloning of a somatostatin receptor (SSTR) subtype from a rat genomic library designated rSSTR4. In the present study, we report the cloning of the human SSTR4 gene. A human genomic library was screened with a 1.2 kb fragment of rSSTR4 containing the full open reading frame and a genomic clone, hSSTR4, was isolated. The deduced amino acid sequence of this clone encoded a protein of 388 amino acids and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SSTR4 sequences demonstrated 89% identity. In addition, the sequence of hSSTR4 shows 61%, 46%, and 47% sequence identity with previously identified isoforms hSSTR1, hSSTR2, and hSSTR3, respectively.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1673</identifier><identifier>PMID: 8512564</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cell Membrane - metabolism ; Cloning, Molecular - methods ; DNA ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Genomic Library ; Humans ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Polymerase Chain Reaction ; Rats ; Receptors, Somatostatin - biosynthesis ; Receptors, Somatostatin - genetics ; Sequence Homology, Amino Acid</subject><ispartof>Biochemical and biophysical research communications, 1993-06, Vol.193 (2), p.648-652</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-140985d18afcb029ab8d6e94b7c823461a97ab077c9ba20f4100478394fd63803</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1993.1673$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4773596$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8512564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Y.</creatorcontrib><creatorcontrib>Song, J.F.</creatorcontrib><creatorcontrib>Bruno, J.F.</creatorcontrib><creatorcontrib>Berelowitz, M.</creatorcontrib><title>Molecular Cloning and Sequencing of a Human Somatostatin Receptor, hSSTR4</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We recently reported the cloning of a somatostatin receptor (SSTR) subtype from a rat genomic library designated rSSTR4. In the present study, we report the cloning of the human SSTR4 gene. A human genomic library was screened with a 1.2 kb fragment of rSSTR4 containing the full open reading frame and a genomic clone, hSSTR4, was isolated. The deduced amino acid sequence of this clone encoded a protein of 388 amino acids and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SSTR4 sequences demonstrated 89% identity. In addition, the sequence of hSSTR4 shows 61%, 46%, and 47% sequence identity with previously identified isoforms hSSTR1, hSSTR2, and hSSTR3, respectively.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - metabolism</subject><subject>Cloning, Molecular - methods</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Genomic Library</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>Receptors, Somatostatin - biosynthesis</subject><subject>Receptors, Somatostatin - genetics</subject><subject>Sequence Homology, Amino Acid</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUx4Moc_64ehN6EE92Jk2aNEcZ6gYTYZvgLbymqVbaZiat4H9v6sZu4unxeJ_v470PQhcETwjG_DbPnZ4QKemEcEEP0JhgieOEYHaIxjgQcSLJ6zE68f4DY0IYlyM0ylKSpJyN0fzJ1kb3NbhoWtu2at8iaItoZT570-qhtWUE0axvoI1WtoHO-g66qo2WRptNZ91N9L5arZfsDB2VUHtzvqun6OXhfj2dxYvnx_n0bhFrKmUXE4ZllhYkg1LnOJGQZwU3kuVCZwllnIAUkGMhtMwhwSULXzKRUcnKgtMM01N0vd27cTYc6TvVVF6buobW2N4rkQaa8-RfMPhiPP0FJ1tQO-u9M6XauKoB960IVoNkNUhWg-QhREPgcre5zxtT7PGd1TC_2s3Ba6hLB0Gl32NMCJpKHrBsi5mg66syTnldBeumqJzRnSps9dcFP1G9ldI</recordid><startdate>19930615</startdate><enddate>19930615</enddate><creator>Xu, Y.</creator><creator>Song, J.F.</creator><creator>Bruno, J.F.</creator><creator>Berelowitz, M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930615</creationdate><title>Molecular Cloning and Sequencing of a Human Somatostatin Receptor, hSSTR4</title><author>Xu, Y. ; Song, J.F. ; Bruno, J.F. ; Berelowitz, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-140985d18afcb029ab8d6e94b7c823461a97ab077c9ba20f4100478394fd63803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - metabolism</topic><topic>Cloning, Molecular - methods</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Genomic Library</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Receptors, Somatostatin - biosynthesis</topic><topic>Receptors, Somatostatin - genetics</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Y.</creatorcontrib><creatorcontrib>Song, J.F.</creatorcontrib><creatorcontrib>Bruno, J.F.</creatorcontrib><creatorcontrib>Berelowitz, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Y.</au><au>Song, J.F.</au><au>Bruno, J.F.</au><au>Berelowitz, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning and Sequencing of a Human Somatostatin Receptor, hSSTR4</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-06-15</date><risdate>1993</risdate><volume>193</volume><issue>2</issue><spage>648</spage><epage>652</epage><pages>648-652</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>We recently reported the cloning of a somatostatin receptor (SSTR) subtype from a rat genomic library designated rSSTR4. In the present study, we report the cloning of the human SSTR4 gene. A human genomic library was screened with a 1.2 kb fragment of rSSTR4 containing the full open reading frame and a genomic clone, hSSTR4, was isolated. The deduced amino acid sequence of this clone encoded a protein of 388 amino acids and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SSTR4 sequences demonstrated 89% identity. In addition, the sequence of hSSTR4 shows 61%, 46%, and 47% sequence identity with previously identified isoforms hSSTR1, hSSTR2, and hSSTR3, respectively.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8512564</pmid><doi>10.1006/bbrc.1993.1673</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1993-06, Vol.193 (2), p.648-652 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75783662 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cell Membrane - metabolism Cloning, Molecular - methods DNA Fundamental and applied biological sciences. Psychology Genes. Genome Genomic Library Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Polymerase Chain Reaction Rats Receptors, Somatostatin - biosynthesis Receptors, Somatostatin - genetics Sequence Homology, Amino Acid |
| title | Molecular Cloning and Sequencing of a Human Somatostatin Receptor, hSSTR4 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T09%3A47%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Cloning%20and%20Sequencing%20of%20a%20Human%20Somatostatin%20Receptor,%20hSSTR4&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Xu,%20Y.&rft.date=1993-06-15&rft.volume=193&rft.issue=2&rft.spage=648&rft.epage=652&rft.pages=648-652&rft.issn=0006-291X&rft.eissn=1090-2104&rft.coden=BBRCA9&rft_id=info:doi/10.1006/bbrc.1993.1673&rft_dat=%3Cproquest_cross%3E16746562%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16746562&rft_id=info:pmid/8512564&rft_els_id=S0006291X83716736&rfr_iscdi=true |