Glutathione S-Transferase π Facilitates the Excretion of Arsenic from Arsenic-Resistant Chinese Hamster Ovary Cells
We have previously demonstrated that glutathione S-transferase π (GSTπ) is overexpressed in SA7 cells, an arsenic resistant cell line derived from Chinese hamster ovary (CHO) cells. Our present results show that SA7 cells accumulate less arsenic than parental CHO cells and partially revertant SA7N c...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-05, Vol.192 (3), p.1093-1099 |
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| creator | Wang, H.F. Lee, T.C. |
| description | We have previously demonstrated that glutathione S-transferase π (GSTπ) is overexpressed in SA7 cells, an arsenic resistant cell line derived from Chinese hamster ovary (CHO) cells. Our present results show that SA7 cells accumulate less arsenic than parental CHO cells and partially revertant SA7N cells. The lower levels of arsenic accumulation in SA7 cells resulted from their faster excretion rates. However, the excretion of arsenic from SA7 cells was significantly inhibited by the GST inhibitors ethacrynic acid and Cibacron blue. Furthermore, when GSTπ levels in SA7N cells were re-elevated by zinc sulfate pretreatment, arsenic accumulation decreased and arsenic excretion increased to levels similar to those in SA7 cells. These results suggest that GST7π can facilitate the excretion of arsenic. Such facilitation by GSTπ is unlikely to be associated with multi-drug resistant P-glycoprotein, since no overexpression of P-glycoprotein was detected in SA7N and SA7 cells. |
| doi_str_mv | 10.1006/bbrc.1993.1529 |
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Our present results show that SA7 cells accumulate less arsenic than parental CHO cells and partially revertant SA7N cells. The lower levels of arsenic accumulation in SA7 cells resulted from their faster excretion rates. However, the excretion of arsenic from SA7 cells was significantly inhibited by the GST inhibitors ethacrynic acid and Cibacron blue. Furthermore, when GSTπ levels in SA7N cells were re-elevated by zinc sulfate pretreatment, arsenic accumulation decreased and arsenic excretion increased to levels similar to those in SA7 cells. These results suggest that GST7π can facilitate the excretion of arsenic. Such facilitation by GSTπ is unlikely to be associated with multi-drug resistant P-glycoprotein, since no overexpression of P-glycoprotein was detected in SA7N and SA7 cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1529</identifier><identifier>PMID: 8099479</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Arsenic - metabolism ; Arsenic - pharmacology ; ATP-Binding Cassette, Sub-Family B, Member 1 ; Biological Transport ; Blotting, Western ; Carrier Proteins - isolation & purification ; Carrier Proteins - metabolism ; CHO Cells ; Clone Cells ; Cricetinae ; Drug Resistance - physiology ; Ethacrynic Acid - pharmacology ; Glutathione Transferase - antagonists & inhibitors ; Glutathione Transferase - metabolism ; Isoenzymes - antagonists & inhibitors ; Isoenzymes - metabolism ; Kinetics ; Membrane Glycoproteins - isolation & purification ; Membrane Glycoproteins - metabolism ; Sulfates - pharmacology ; Time Factors ; Triazines - pharmacology ; Zinc - pharmacology ; Zinc Sulfate</subject><ispartof>Biochemical and biophysical research communications, 1993-05, Vol.192 (3), p.1093-1099</ispartof><rights>1993 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-d677f19aba32056b146311813554a661176ab0077c19025b4f523c99a35647253</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X83715299$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8099479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, H.F.</creatorcontrib><creatorcontrib>Lee, T.C.</creatorcontrib><title>Glutathione S-Transferase π Facilitates the Excretion of Arsenic from Arsenic-Resistant Chinese Hamster Ovary Cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We have previously demonstrated that glutathione S-transferase π (GSTπ) is overexpressed in SA7 cells, an arsenic resistant cell line derived from Chinese hamster ovary (CHO) cells. Our present results show that SA7 cells accumulate less arsenic than parental CHO cells and partially revertant SA7N cells. The lower levels of arsenic accumulation in SA7 cells resulted from their faster excretion rates. However, the excretion of arsenic from SA7 cells was significantly inhibited by the GST inhibitors ethacrynic acid and Cibacron blue. Furthermore, when GSTπ levels in SA7N cells were re-elevated by zinc sulfate pretreatment, arsenic accumulation decreased and arsenic excretion increased to levels similar to those in SA7 cells. These results suggest that GST7π can facilitate the excretion of arsenic. Such facilitation by GSTπ is unlikely to be associated with multi-drug resistant P-glycoprotein, since no overexpression of P-glycoprotein was detected in SA7N and SA7 cells.</description><subject>Animals</subject><subject>Arsenic - metabolism</subject><subject>Arsenic - pharmacology</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1</subject><subject>Biological Transport</subject><subject>Blotting, Western</subject><subject>Carrier Proteins - isolation & purification</subject><subject>Carrier Proteins - metabolism</subject><subject>CHO Cells</subject><subject>Clone Cells</subject><subject>Cricetinae</subject><subject>Drug Resistance - physiology</subject><subject>Ethacrynic Acid - pharmacology</subject><subject>Glutathione Transferase - antagonists & inhibitors</subject><subject>Glutathione Transferase - metabolism</subject><subject>Isoenzymes - antagonists & inhibitors</subject><subject>Isoenzymes - metabolism</subject><subject>Kinetics</subject><subject>Membrane Glycoproteins - isolation & purification</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Sulfates - pharmacology</subject><subject>Time Factors</subject><subject>Triazines - pharmacology</subject><subject>Zinc - pharmacology</subject><subject>Zinc Sulfate</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVJSdy0194COuW2rma1klbHYOKkEAi0KfQmtPIsVtmPRCOH9NZ_2L9UGbu5lZ6EeB-9zOhh7COIJQihP3VdCkuwVi5B1fYNW4CwoqpBNCdsIQpR1Ra-n7F3RD-EAGi0PWWnrbC2MXbB8s2wyz5v4zwh_1o9JD9Rj8kT8t-_-NqHOMSSI_G8RX79EhLmwvK551eJcIqB92ke_16qL0iRsp8yX23jhKXm1o-UMfH7Z59-8hUOA71nb3s_EH44nufs2_r6YXVb3d3ffF5d3VVBGpGrjTamB-s7L2uhdFdmlwAtSKUarzWA0b4TwpgAVtSqa3pVy2Ctl0o3plbynF0eeh_T_LRDym6MFMoEfsJ5R84o08patv8FQSvbKCELuDyAIc1ECXv3mOJYFnMg3N6H2_twex9u76M8uDg277oRN6_4UUDJ20OO5R-eIyZHIeIUcBMThuw2c_xX9R-leZlM</recordid><startdate>19930514</startdate><enddate>19930514</enddate><creator>Wang, H.F.</creator><creator>Lee, T.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19930514</creationdate><title>Glutathione S-Transferase π Facilitates the Excretion of Arsenic from Arsenic-Resistant Chinese Hamster Ovary Cells</title><author>Wang, H.F. ; Lee, T.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-d677f19aba32056b146311813554a661176ab0077c19025b4f523c99a35647253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Arsenic - metabolism</topic><topic>Arsenic - pharmacology</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1</topic><topic>Biological Transport</topic><topic>Blotting, Western</topic><topic>Carrier Proteins - isolation & purification</topic><topic>Carrier Proteins - metabolism</topic><topic>CHO Cells</topic><topic>Clone Cells</topic><topic>Cricetinae</topic><topic>Drug Resistance - physiology</topic><topic>Ethacrynic Acid - pharmacology</topic><topic>Glutathione Transferase - antagonists & inhibitors</topic><topic>Glutathione Transferase - metabolism</topic><topic>Isoenzymes - antagonists & inhibitors</topic><topic>Isoenzymes - metabolism</topic><topic>Kinetics</topic><topic>Membrane Glycoproteins - isolation & purification</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Sulfates - pharmacology</topic><topic>Time Factors</topic><topic>Triazines - pharmacology</topic><topic>Zinc - pharmacology</topic><topic>Zinc Sulfate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, H.F.</creatorcontrib><creatorcontrib>Lee, T.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, H.F.</au><au>Lee, T.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutathione S-Transferase π Facilitates the Excretion of Arsenic from Arsenic-Resistant Chinese Hamster Ovary Cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-05-14</date><risdate>1993</risdate><volume>192</volume><issue>3</issue><spage>1093</spage><epage>1099</epage><pages>1093-1099</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We have previously demonstrated that glutathione S-transferase π (GSTπ) is overexpressed in SA7 cells, an arsenic resistant cell line derived from Chinese hamster ovary (CHO) cells. Our present results show that SA7 cells accumulate less arsenic than parental CHO cells and partially revertant SA7N cells. The lower levels of arsenic accumulation in SA7 cells resulted from their faster excretion rates. However, the excretion of arsenic from SA7 cells was significantly inhibited by the GST inhibitors ethacrynic acid and Cibacron blue. Furthermore, when GSTπ levels in SA7N cells were re-elevated by zinc sulfate pretreatment, arsenic accumulation decreased and arsenic excretion increased to levels similar to those in SA7 cells. These results suggest that GST7π can facilitate the excretion of arsenic. Such facilitation by GSTπ is unlikely to be associated with multi-drug resistant P-glycoprotein, since no overexpression of P-glycoprotein was detected in SA7N and SA7 cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8099479</pmid><doi>10.1006/bbrc.1993.1529</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1993-05, Vol.192 (3), p.1093-1099 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Arsenic - metabolism Arsenic - pharmacology ATP-Binding Cassette, Sub-Family B, Member 1 Biological Transport Blotting, Western Carrier Proteins - isolation & purification Carrier Proteins - metabolism CHO Cells Clone Cells Cricetinae Drug Resistance - physiology Ethacrynic Acid - pharmacology Glutathione Transferase - antagonists & inhibitors Glutathione Transferase - metabolism Isoenzymes - antagonists & inhibitors Isoenzymes - metabolism Kinetics Membrane Glycoproteins - isolation & purification Membrane Glycoproteins - metabolism Sulfates - pharmacology Time Factors Triazines - pharmacology Zinc - pharmacology Zinc Sulfate |
| title | Glutathione S-Transferase π Facilitates the Excretion of Arsenic from Arsenic-Resistant Chinese Hamster Ovary Cells |
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