Rapid Induction and Isolation of Focal Adhesion Complexes

Focal adhesion complexes (FACs) containing integrin β1, talin, vinculin, talin, α-actinin, and paxillin formed within 15 min when round cells bound magnetic microbeads coated with integrin ligands, such as fibronectin or RGD-containing peptide, but not when coated with acetylated-low density lipopro...

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Veröffentlicht in:	Biochemical and biophysical research communications 1993-06, Vol.193 (2), p.571-578
Hauptverfasser:	Plopper, G., Ingber, D.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinin - isolation & purification Actinin - metabolism Actins - isolation & purification Actins - metabolism Animals Biological and medical sciences Blotting, Western Capillaries Cattle Cell Adhesion Cell interactions, adhesion Cell Membrane - metabolism Cells, Cultured Cytoskeletal Proteins - isolation & purification Cytoskeletal Proteins - metabolism Endothelium, Vascular - metabolism Extracellular Matrix Proteins - isolation & purification Extracellular Matrix Proteins - metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Integrin beta1 Integrins - isolation & purification Integrins - metabolism Molecular and cellular biology Paxillin Phosphoproteins - isolation & purification Phosphoproteins - metabolism Space life sciences Talin - isolation & purification Talin - metabolism Vinculin - isolation & purification Vinculin - metabolism
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