Relationships between Composition and Cellular Uptake of Lipoproteins from Normal and Hyperlipemic Swine
Young male swine fed a high fat, 1% cholesterol diet for 6 weeks developed a hyperlipemia accompanied by alterations in the composition of the very low density, intermediate density and low density lipoproteins (VLDL, IDL, LDL). High density lipoprotein (HDL) composition remained relatively constant...
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| Veröffentlicht in: | The Journal of nutrition 1984-12, Vol.114 (12), p.2373-2382 |
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| creator | Shireman, Rachel B. Coffey, M. Terry |
| description | Young male swine fed a high fat, 1% cholesterol diet for 6 weeks developed a hyperlipemia accompanied by alterations in the composition of the very low density, intermediate density and low density lipoproteins (VLDL, IDL, LDL). High density lipoprotein (HDL) composition remained relatively constant. In addition to compositional analysis, determination of sedimentation coefficients indicated the LDL of hyperlipemic animals was of slightly larger size. The in vitro cellular uptake of normal swine and human 125I-labeled LDL by cultured human fibroblasts was similar in time- and concentration-dependent studies. The receptor-mediated uptake of normal swine 125I-labeled LDL, as well as IDL and VLDL, was significantly greater than that of hyperlipemic swine lipoproteins. Nonspecific uptake of normal and hyperlipemic 125I-labeled LDL was similar, as determined in studies with mutant receptor-negative familial hypercholesterolemic fibroblasts. It is hypothesized that the size of the lipoprotein particle may be a determinant in cellular uptake by physically limiting the number of particles that bind to the receptor site for uptake by the cell. |
| doi_str_mv | 10.1093/jn/114.12.2373 |
| format | Article |
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Nonspecific uptake of normal and hyperlipemic 125I-labeled LDL was similar, as determined in studies with mutant receptor-negative familial hypercholesterolemic fibroblasts. It is hypothesized that the size of the lipoprotein particle may be a determinant in cellular uptake by physically limiting the number of particles that bind to the receptor site for uptake by the cell.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/114.12.2373</identifier><identifier>PMID: 6502280</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Body Weight ; cellular receptors for low density lipoproteins ; CERDO ; Cholesterol - blood ; Dietary Fats - metabolism ; dietary-induced hyperlipemia ; DIETS ; Fibroblasts - metabolism ; Fundamental and applied biological sciences. 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Terry</creatorcontrib><title>Relationships between Composition and Cellular Uptake of Lipoproteins from Normal and Hyperlipemic Swine</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>Young male swine fed a high fat, 1% cholesterol diet for 6 weeks developed a hyperlipemia accompanied by alterations in the composition of the very low density, intermediate density and low density lipoproteins (VLDL, IDL, LDL). High density lipoprotein (HDL) composition remained relatively constant. In addition to compositional analysis, determination of sedimentation coefficients indicated the LDL of hyperlipemic animals was of slightly larger size. The in vitro cellular uptake of normal swine and human 125I-labeled LDL by cultured human fibroblasts was similar in time- and concentration-dependent studies. The receptor-mediated uptake of normal swine 125I-labeled LDL, as well as IDL and VLDL, was significantly greater than that of hyperlipemic swine lipoproteins. Nonspecific uptake of normal and hyperlipemic 125I-labeled LDL was similar, as determined in studies with mutant receptor-negative familial hypercholesterolemic fibroblasts. It is hypothesized that the size of the lipoprotein particle may be a determinant in cellular uptake by physically limiting the number of particles that bind to the receptor site for uptake by the cell.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body Weight</subject><subject>cellular receptors for low density lipoproteins</subject><subject>CERDO</subject><subject>Cholesterol - blood</subject><subject>Dietary Fats - metabolism</subject><subject>dietary-induced hyperlipemia</subject><subject>DIETS</subject><subject>Fibroblasts - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>HYPERLIPEMIA</subject><subject>Hyperlipidemias - etiology</subject><subject>intermediate density lipoproteins</subject><subject>Iodine Radioisotopes</subject><subject>Lipids. Glycolipids</subject><subject>Lipoproteins, HDL - blood</subject><subject>Lipoproteins, HDL - metabolism</subject><subject>Lipoproteins, LDL - blood</subject><subject>Lipoproteins, LDL - metabolism</subject><subject>Lipoproteins, VLDL - blood</subject><subject>Lipoproteins, VLDL - metabolism</subject><subject>low and very low density lipoproteins</subject><subject>Male</subject><subject>Metabolisms and neurohumoral controls</subject><subject>PORCIN</subject><subject>SWINE</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFv0zAUhy0EGt3gygEJyQe0Wzo_J7GdI6qAIVUgMXq2HOeZuSR2sFOm_fe4tNqNkw_f56efPkLeAFsD6-qbfbgBaNbA17yW9TOygraBSgBjz8mKMc6rGoR4SS5z3jPGoOnUBbkQbSGKrcj9dxzN4mPI937OtMflATHQTZzmmP0RUBMGusFxPIwm0d28mF9Io6NbP8c5xQV9yNSlONGvMU1m_OffPs6YRj_j5C29e_ABX5EXzowZX5_fK7L79PHH5rbafvv8ZfNhW9mGy6XquOUOheJGwCDUwB2gwm7gFkQ7SNOrRnSiZqrvatko1_dO4mAMFwgCUNRX5Pp0t2z7fcC86MlnW-abgPGQtWylbFvZFHF9Em2KOSd0ek5-MulRA9PHtHofdEmrgetj2vLh3fnyoZ9weNLPLQt_f-YmWzO6ZIL1-UnrQEnWQNHenjRnojY_U1F2d0qAbJvjKHWCWBL98Zh0th6DxcEntIseov_fvL9XDJ2G</recordid><startdate>198412</startdate><enddate>198412</enddate><creator>Shireman, Rachel B.</creator><creator>Coffey, M. 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Terry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-92c2fe682a61d68d2f1e8e9d2c165d7ab84696308b93748fbbf7edaa26e161e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body Weight</topic><topic>cellular receptors for low density lipoproteins</topic><topic>CERDO</topic><topic>Cholesterol - blood</topic><topic>Dietary Fats - metabolism</topic><topic>dietary-induced hyperlipemia</topic><topic>DIETS</topic><topic>Fibroblasts - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>HYPERLIPEMIA</topic><topic>Hyperlipidemias - etiology</topic><topic>intermediate density lipoproteins</topic><topic>Iodine Radioisotopes</topic><topic>Lipids. Glycolipids</topic><topic>Lipoproteins, HDL - blood</topic><topic>Lipoproteins, HDL - metabolism</topic><topic>Lipoproteins, LDL - blood</topic><topic>Lipoproteins, LDL - metabolism</topic><topic>Lipoproteins, VLDL - blood</topic><topic>Lipoproteins, VLDL - metabolism</topic><topic>low and very low density lipoproteins</topic><topic>Male</topic><topic>Metabolisms and neurohumoral controls</topic><topic>PORCIN</topic><topic>SWINE</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shireman, Rachel B.</creatorcontrib><creatorcontrib>Coffey, M. 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Terry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationships between Composition and Cellular Uptake of Lipoproteins from Normal and Hyperlipemic Swine</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1984-12</date><risdate>1984</risdate><volume>114</volume><issue>12</issue><spage>2373</spage><epage>2382</epage><pages>2373-2382</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>Young male swine fed a high fat, 1% cholesterol diet for 6 weeks developed a hyperlipemia accompanied by alterations in the composition of the very low density, intermediate density and low density lipoproteins (VLDL, IDL, LDL). High density lipoprotein (HDL) composition remained relatively constant. In addition to compositional analysis, determination of sedimentation coefficients indicated the LDL of hyperlipemic animals was of slightly larger size. The in vitro cellular uptake of normal swine and human 125I-labeled LDL by cultured human fibroblasts was similar in time- and concentration-dependent studies. The receptor-mediated uptake of normal swine 125I-labeled LDL, as well as IDL and VLDL, was significantly greater than that of hyperlipemic swine lipoproteins. Nonspecific uptake of normal and hyperlipemic 125I-labeled LDL was similar, as determined in studies with mutant receptor-negative familial hypercholesterolemic fibroblasts. It is hypothesized that the size of the lipoprotein particle may be a determinant in cellular uptake by physically limiting the number of particles that bind to the receptor site for uptake by the cell.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>6502280</pmid><doi>10.1093/jn/114.12.2373</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Body Weight cellular receptors for low density lipoproteins CERDO Cholesterol - blood Dietary Fats - metabolism dietary-induced hyperlipemia DIETS Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Humans HYPERLIPEMIA Hyperlipidemias - etiology intermediate density lipoproteins Iodine Radioisotopes Lipids. Glycolipids Lipoproteins, HDL - blood Lipoproteins, HDL - metabolism Lipoproteins, LDL - blood Lipoproteins, LDL - metabolism Lipoproteins, VLDL - blood Lipoproteins, VLDL - metabolism low and very low density lipoproteins Male Metabolisms and neurohumoral controls PORCIN SWINE Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Relationships between Composition and Cellular Uptake of Lipoproteins from Normal and Hyperlipemic Swine |
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