The glycosylphosphatidylinositol-anchored lymphocyte antigen CDw52 is associated with the epididymal maturation of human spermatozoa
The CAMPATH-1 (CDw52) antigen is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein with a mature peptide comprising only 12 amino acids. It is abundantly expressed on human lymphocytes and is an unusually good target for complement-mediated cell lysis. The immunosuppressive and lympho...
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| Veröffentlicht in: | Journal of reproductive immunology 1993-03, Vol.23 (2), p.189-205 |
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| creator | Hale, Geoffrey Rye, Philip D. Warford, Anthony Lauder, Ian Brito-Babapulle, Adrian |
| description | The CAMPATH-1 (CDw52) antigen is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein with a mature peptide comprising only 12 amino acids. It is abundantly expressed on human lymphocytes and is an unusually good target for complement-mediated cell lysis. The immunosuppressive and lymphocyte-depleting effects of CAMPATH-1 antibodies are being tested in a variety of diseases. Here we show that the antigen is also expressed at a high level in the male reproductive system, being found in the epididymis, seminal vesicle, seminal plasma and on the surface of mature (but not testicular) spermatozoa. Its possible transfer from epithelial cells in the epididymis to maturing sperm may represent a novel method of acquisition of cell surface antigens. In the presence of human complement, CAMPATH-1 antibodies inhibited the motility of washed sperm. However, seminal plasma blocks antibody binding and can protect sperm from this cytotoxic effect. |
| doi_str_mv | 10.1016/0165-0378(93)90007-5 |
| format | Article |
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It is abundantly expressed on human lymphocytes and is an unusually good target for complement-mediated cell lysis. The immunosuppressive and lymphocyte-depleting effects of CAMPATH-1 antibodies are being tested in a variety of diseases. Here we show that the antigen is also expressed at a high level in the male reproductive system, being found in the epididymis, seminal vesicle, seminal plasma and on the surface of mature (but not testicular) spermatozoa. Its possible transfer from epithelial cells in the epididymis to maturing sperm may represent a novel method of acquisition of cell surface antigens. In the presence of human complement, CAMPATH-1 antibodies inhibited the motility of washed sperm. However, seminal plasma blocks antibody binding and can protect sperm from this cytotoxic effect.</description><identifier>ISSN: 0165-0378</identifier><identifier>EISSN: 1872-7603</identifier><identifier>DOI: 10.1016/0165-0378(93)90007-5</identifier><identifier>PMID: 7685389</identifier><identifier>CODEN: JRIMDR</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Anti-sperm monoclonal antibodies ; Antigens, CD - analysis ; Antigens, CD - biosynthesis ; Antigens, CD - immunology ; Antigens, Neoplasm ; Biological and medical sciences ; Blotting, Western ; CAMPATH-1 ; CD52 Antigen ; CD59 Antigens ; CDw52 antigen ; Cytotoxicity, Immunologic ; Dose-Response Relationship, Immunologic ; epididymis ; Epididymis - immunology ; Erythrocytes - immunology ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; GPI anchor ; Histocompatibility Antigens Class I - biosynthesis ; Humans ; Leukocyte Common Antigens - biosynthesis ; Lymphocytes - immunology ; Male ; Mammalian male genital system ; Membrane Glycoproteins - biosynthesis ; Middle Aged ; Morphology. Physiology ; Prostate - immunology ; Semen - immunology ; seminal plasma ; Seminal Vesicles - immunology ; Sperm Motility - immunology ; Spermatogenesis - immunology ; spermatozoa ; Spermatozoa - immunology ; Vertebrates: reproduction</subject><ispartof>Journal of reproductive immunology, 1993-03, Vol.23 (2), p.189-205</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-24937340f9b9c74aea5fa91a59c1fdd68cd68cdfb2d3e0191db14b87975f2b253</citedby><cites>FETCH-LOGICAL-c417t-24937340f9b9c74aea5fa91a59c1fdd68cd68cdfb2d3e0191db14b87975f2b253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0165037893900075$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4677862$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7685389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hale, Geoffrey</creatorcontrib><creatorcontrib>Rye, Philip D.</creatorcontrib><creatorcontrib>Warford, Anthony</creatorcontrib><creatorcontrib>Lauder, Ian</creatorcontrib><creatorcontrib>Brito-Babapulle, Adrian</creatorcontrib><title>The glycosylphosphatidylinositol-anchored lymphocyte antigen CDw52 is associated with the epididymal maturation of human spermatozoa</title><title>Journal of reproductive immunology</title><addtitle>J Reprod Immunol</addtitle><description>The CAMPATH-1 (CDw52) antigen is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein with a mature peptide comprising only 12 amino acids. It is abundantly expressed on human lymphocytes and is an unusually good target for complement-mediated cell lysis. The immunosuppressive and lymphocyte-depleting effects of CAMPATH-1 antibodies are being tested in a variety of diseases. Here we show that the antigen is also expressed at a high level in the male reproductive system, being found in the epididymis, seminal vesicle, seminal plasma and on the surface of mature (but not testicular) spermatozoa. Its possible transfer from epithelial cells in the epididymis to maturing sperm may represent a novel method of acquisition of cell surface antigens. In the presence of human complement, CAMPATH-1 antibodies inhibited the motility of washed sperm. However, seminal plasma blocks antibody binding and can protect sperm from this cytotoxic effect.</description><subject>Anti-sperm monoclonal antibodies</subject><subject>Antigens, CD - analysis</subject><subject>Antigens, CD - biosynthesis</subject><subject>Antigens, CD - immunology</subject><subject>Antigens, Neoplasm</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>CAMPATH-1</subject><subject>CD52 Antigen</subject><subject>CD59 Antigens</subject><subject>CDw52 antigen</subject><subject>Cytotoxicity, Immunologic</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>epididymis</subject><subject>Epididymis - immunology</subject><subject>Erythrocytes - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>GPI anchor</subject><subject>Histocompatibility Antigens Class I - biosynthesis</subject><subject>Humans</subject><subject>Leukocyte Common Antigens - biosynthesis</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Membrane Glycoproteins - biosynthesis</subject><subject>Middle Aged</subject><subject>Morphology. Physiology</subject><subject>Prostate - immunology</subject><subject>Semen - immunology</subject><subject>seminal plasma</subject><subject>Seminal Vesicles - immunology</subject><subject>Sperm Motility - immunology</subject><subject>Spermatogenesis - immunology</subject><subject>spermatozoa</subject><subject>Spermatozoa - immunology</subject><subject>Vertebrates: reproduction</subject><issn>0165-0378</issn><issn>1872-7603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1TAUhoMo43X0HyhkIaKLatI2TbMZkOsnDLgZ1yFNTqaRtKlJ6lDX_nBz517uUheHAzlPXg7nQeg5JW8pod27UqwiDe9fi-aNIITwij1AO9rzuuIdaR6i3Rl5jJ6k9IMQyomgF-iCdz1rerFDf25GwLd-0yFtfhlDWkaVndm8m0NyOfhKzXoMEQz221QAvWXAas7uFma8_3DHauwSVikF7VQu2J3LI84lFRZnStKkPJ5UXmPJDTMOFo_rpGacFojlPfwO6il6ZJVP8OzUL9H3Tx9v9l-q62-fv-7fX1e6pTxXdSsa3rTEikFo3ipQzCpBFROaWmO6Xt-XHWrTAKGCmoG2Q88FZ7YeatZcolfH3CWGnyukLCeXNHivZghrkpzxTnSk-y9Iy_kIZbSA7RHUMaQUwcoluknFTVIiD5bkQYE8KJCikfeW5GGRF6f8dZjAnD-dtJT5y9NcJa28jUWCS2es7Tjvu7pgV0cMytF-OYgyaQezBuMi6CxNcP_e4y_XB7GC</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>Hale, Geoffrey</creator><creator>Rye, Philip D.</creator><creator>Warford, Anthony</creator><creator>Lauder, Ian</creator><creator>Brito-Babapulle, Adrian</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>The glycosylphosphatidylinositol-anchored lymphocyte antigen CDw52 is associated with the epididymal maturation of human spermatozoa</title><author>Hale, Geoffrey ; Rye, Philip D. ; Warford, Anthony ; Lauder, Ian ; Brito-Babapulle, Adrian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-24937340f9b9c74aea5fa91a59c1fdd68cd68cdfb2d3e0191db14b87975f2b253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Anti-sperm monoclonal antibodies</topic><topic>Antigens, CD - analysis</topic><topic>Antigens, CD - biosynthesis</topic><topic>Antigens, CD - immunology</topic><topic>Antigens, Neoplasm</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>CAMPATH-1</topic><topic>CD52 Antigen</topic><topic>CD59 Antigens</topic><topic>CDw52 antigen</topic><topic>Cytotoxicity, Immunologic</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>epididymis</topic><topic>Epididymis - immunology</topic><topic>Erythrocytes - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins</topic><topic>GPI anchor</topic><topic>Histocompatibility Antigens Class I - biosynthesis</topic><topic>Humans</topic><topic>Leukocyte Common Antigens - biosynthesis</topic><topic>Lymphocytes - immunology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Membrane Glycoproteins - biosynthesis</topic><topic>Middle Aged</topic><topic>Morphology. Physiology</topic><topic>Prostate - immunology</topic><topic>Semen - immunology</topic><topic>seminal plasma</topic><topic>Seminal Vesicles - immunology</topic><topic>Sperm Motility - immunology</topic><topic>Spermatogenesis - immunology</topic><topic>spermatozoa</topic><topic>Spermatozoa - immunology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hale, Geoffrey</creatorcontrib><creatorcontrib>Rye, Philip D.</creatorcontrib><creatorcontrib>Warford, Anthony</creatorcontrib><creatorcontrib>Lauder, Ian</creatorcontrib><creatorcontrib>Brito-Babapulle, Adrian</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of reproductive immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hale, Geoffrey</au><au>Rye, Philip D.</au><au>Warford, Anthony</au><au>Lauder, Ian</au><au>Brito-Babapulle, Adrian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The glycosylphosphatidylinositol-anchored lymphocyte antigen CDw52 is associated with the epididymal maturation of human spermatozoa</atitle><jtitle>Journal of reproductive immunology</jtitle><addtitle>J Reprod Immunol</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>23</volume><issue>2</issue><spage>189</spage><epage>205</epage><pages>189-205</pages><issn>0165-0378</issn><eissn>1872-7603</eissn><coden>JRIMDR</coden><abstract>The CAMPATH-1 (CDw52) antigen is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein with a mature peptide comprising only 12 amino acids. It is abundantly expressed on human lymphocytes and is an unusually good target for complement-mediated cell lysis. The immunosuppressive and lymphocyte-depleting effects of CAMPATH-1 antibodies are being tested in a variety of diseases. Here we show that the antigen is also expressed at a high level in the male reproductive system, being found in the epididymis, seminal vesicle, seminal plasma and on the surface of mature (but not testicular) spermatozoa. Its possible transfer from epithelial cells in the epididymis to maturing sperm may represent a novel method of acquisition of cell surface antigens. In the presence of human complement, CAMPATH-1 antibodies inhibited the motility of washed sperm. However, seminal plasma blocks antibody binding and can protect sperm from this cytotoxic effect.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>7685389</pmid><doi>10.1016/0165-0378(93)90007-5</doi><tpages>17</tpages></addata></record> |
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| ispartof | Journal of reproductive immunology, 1993-03, Vol.23 (2), p.189-205 |
| issn | 0165-0378 1872-7603 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-sperm monoclonal antibodies Antigens, CD - analysis Antigens, CD - biosynthesis Antigens, CD - immunology Antigens, Neoplasm Biological and medical sciences Blotting, Western CAMPATH-1 CD52 Antigen CD59 Antigens CDw52 antigen Cytotoxicity, Immunologic Dose-Response Relationship, Immunologic epididymis Epididymis - immunology Erythrocytes - immunology Fundamental and applied biological sciences. Psychology Glycoproteins GPI anchor Histocompatibility Antigens Class I - biosynthesis Humans Leukocyte Common Antigens - biosynthesis Lymphocytes - immunology Male Mammalian male genital system Membrane Glycoproteins - biosynthesis Middle Aged Morphology. Physiology Prostate - immunology Semen - immunology seminal plasma Seminal Vesicles - immunology Sperm Motility - immunology Spermatogenesis - immunology spermatozoa Spermatozoa - immunology Vertebrates: reproduction |
| title | The glycosylphosphatidylinositol-anchored lymphocyte antigen CDw52 is associated with the epididymal maturation of human spermatozoa |
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