Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis
We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study...
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| Veröffentlicht in: | Journal of Clinical Microbiology 1993-05, Vol.31 (5), p.1048-1054 |
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| creator | Thierry, D. (Institut Pasteur, Paris, France) Vincent, V Clement, F Guesdon, J.L |
| description | We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- and DT6-derived oligonucleotides were selected for use as primers in a polymerase chain reaction test. Amplification of the DT1 and DT6 sequences may provide the basis for a rapid and reliable assay for the detection of mycobacteria belonging to MAC |
| doi_str_mv | 10.1128/jcm.31.5.1048-1054.1993 |
| format | Article |
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(Institut Pasteur, Paris, France) ; Vincent, V ; Clement, F ; Guesdon, J.L</creator><creatorcontrib>Thierry, D. (Institut Pasteur, Paris, France) ; Vincent, V ; Clement, F ; Guesdon, J.L</creatorcontrib><description>We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- and DT6-derived oligonucleotides were selected for use as primers in a polymerase chain reaction test. Amplification of the DT1 and DT6 sequences may provide the basis for a rapid and reliable assay for the detection of mycobacteria belonging to MAC</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.31.5.1048-1054.1993</identifier><identifier>PMID: 8501206</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; DNA Probes ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Humans ; Microbiology ; Molecular Sequence Data ; MYCOBACTERIUM ; Mycobacterium avium ; Mycobacterium avium - classification ; Mycobacterium avium - genetics ; Mycobacterium avium - isolation & purification ; Mycobacterium avium Complex - genetics ; Mycobacterium avium Complex - isolation & purification ; Mycobacterium avium-intracellulare Infection - diagnosis ; Polymerase Chain Reaction ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; Species Specificity</subject><ispartof>Journal of Clinical Microbiology, 1993-05, Vol.31 (5), p.1048-1054</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-fc926f14dd6b49f81bd1faadae07b4124af125a02011c74012c4dd01ea31eb553</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC262878/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC262878/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4741361$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8501206$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thierry, D. (Institut Pasteur, Paris, France)</creatorcontrib><creatorcontrib>Vincent, V</creatorcontrib><creatorcontrib>Clement, F</creatorcontrib><creatorcontrib>Guesdon, J.L</creatorcontrib><title>Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- and DT6-derived oligonucleotides were selected for use as primers in a polymerase chain reaction test. Amplification of the DT1 and DT6 sequences may provide the basis for a rapid and reliable assay for the detection of mycobacteria belonging to MAC</description><subject>Animals</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Probes</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>MYCOBACTERIUM</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium - classification</subject><subject>Mycobacterium avium - genetics</subject><subject>Mycobacterium avium - isolation & purification</subject><subject>Mycobacterium avium Complex - genetics</subject><subject>Mycobacterium avium Complex - isolation & purification</subject><subject>Mycobacterium avium-intracellulare Infection - diagnosis</subject><subject>Polymerase Chain Reaction</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Species Specificity</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKkPhBZAQRkLsEnwTO4kXXVTlr1KBBVRihXXj2BlXSTzYSVHfHocZjegKWbIX5zvXxz6EvASWAxTN2xs95iXkIgfGmwyY4DlIWT4gG2CyyaqK_XhINoxJkQGU9WPyJMYbxoBzIU7ISSMYFKzakJ-X0Q84Oz9Rb2ncGe2s0_Tdl3NqA_ajmea4Kp_vtG9Rzya4ZaR4-3efOjpvjQt052N07WDoEg11E-0c9pOPLj4ljywO0Tw7nKfk-sP77xefsquvHy8vzq8yLWo2Z1bLorLAu65qubQNtB1YxA4Nq1sOBUcLhUBWMABd85RdJ5aBwRJMK0R5Ss72c3dLO5pOp9gBB7ULbsRwpzw6dV-Z3Fb1_lYVVdHUTfK_OfiD_7WYOKvRRW2GASfjl6hqUQuZ1n9BqHgjBawT6z2oQ_qcYOwxDDC1VqhShaoEJdRaoVorVGuFyfni37ccfYfOkv76oGPUOKSaJu3iEeM1h7KChL3aY1vXb3-7YBTG8f6liXm-Zyx6hX1IY66_SQ5cVKz8A1cZung</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>Thierry, D. 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(Institut Pasteur, Paris, France) ; Vincent, V ; Clement, F ; Guesdon, J.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-fc926f14dd6b49f81bd1faadae07b4124af125a02011c74012c4dd01ea31eb553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA Probes</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>MYCOBACTERIUM</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium - classification</topic><topic>Mycobacterium avium - genetics</topic><topic>Mycobacterium avium - isolation & purification</topic><topic>Mycobacterium avium Complex - genetics</topic><topic>Mycobacterium avium Complex - isolation & purification</topic><topic>Mycobacterium avium-intracellulare Infection - diagnosis</topic><topic>Polymerase Chain Reaction</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thierry, D. 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The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- and DT6-derived oligonucleotides were selected for use as primers in a polymerase chain reaction test. Amplification of the DT1 and DT6 sequences may provide the basis for a rapid and reliable assay for the detection of mycobacteria belonging to MAC</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8501206</pmid><doi>10.1128/jcm.31.5.1048-1054.1993</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences DNA Probes DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Humans Microbiology Molecular Sequence Data MYCOBACTERIUM Mycobacterium avium Mycobacterium avium - classification Mycobacterium avium - genetics Mycobacterium avium - isolation & purification Mycobacterium avium Complex - genetics Mycobacterium avium Complex - isolation & purification Mycobacterium avium-intracellulare Infection - diagnosis Polymerase Chain Reaction SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Species Specificity |
| title | Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis |
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